Protein Kodlanmayan Bölgelerin (UTR) Rolü ve Bu Bölgelerin Haritalanmasında Kullanılan Başlıca Yöntemler

Seda Gözel; Cüneyt Tamer

doi:10.51755/turkvetj.1417084

EN TR

ASSAYS USED IN THE ANALYSIS OF UNTRANSLATED REGIONS OF VIRUSES

Abstract

Untranslated regions (UTRs) of virus genomes are known to play crucial roles in the post- transcriptional regulation of gene expression, including modulation of the transport of mRNAs out of the nucleus and of translation efficiency, subcellular localization and stability. The molecu-lar profiling of many diseases has shown that the untranslated regions of mRNAs play a more significant role in disease progression and susceptibility than previously thought. Therefore, the methods used in the mapping and detection of these regions continue to gain importance. De-pending on technology development, each test method makes significant progress through uni-versity research and commercial companies. Therefore, it provides more detailed information about the mutations that occur in these regions and their effects on the formation of the disease. In this study, the tests used to detect untranslated regions are described. These tests include Pri-mer Extension Assay, S1 Nuclease Assay,RNAse Protection Assay, and Rapid Amplification of cDNA Ends Assay. These assays can be used to map the 5’ and 3’ terminus of an RNA and to quantitate the amount of a given RNA by extending a primer using reverse transcriptase.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

Veteriner Viroloji

Bölüm

Derleme

Yazarlar

Seda Gözel ^*
0000-0003-2976-5245
Türkiye

Cüneyt Tamer
0000-0003-3240-8425
Türkiye

Erken Görünüm Tarihi

30 Haziran 2024

Yayımlanma Tarihi

30 Haziran 2024

Gönderilme Tarihi

10 Ocak 2024

Kabul Tarihi

26 Şubat 2024

Yayımlandığı Sayı

Yıl 1970 Cilt: 6 Sayı: 1

DOI

https://doi.org/10.51755/turkvetj.1417084

IZ

https://izlik.org/JA43TW68SA

Kaynak Göster

RIS / Bibtex

APA

Gözel, S., & Tamer, C. (2024). Protein Kodlanmayan Bölgelerin (UTR) Rolü ve Bu Bölgelerin Haritalanmasında Kullanılan Başlıca Yöntemler. Turkish Veterinary Journal, 6(1), 12-17. https://doi.org/10.51755/turkvetj.1417084

Protein Kodlanmayan Bölgelerin (UTR) Rolü ve Bu Bölgelerin Haritalanmasında Kullanılan Başlıca Yöntemler

ASSAYS USED IN THE ANALYSIS OF UNTRANSLATED REGIONS OF VIRUSES

Abstract

Keywords