Protein Kodlanmayan Bölgelerin (UTR) Rolü ve Bu Bölgelerin Haritalanmasında Kullanılan Başlıca Yöntemler

Seda Gözel; Cüneyt Tamer

doi:10.51755/turkvetj.1417084

EN TR

ASSAYS USED IN THE ANALYSIS OF UNTRANSLATED REGIONS OF VIRUSES

Abstract

Untranslated regions (UTRs) of virus genomes are known to play crucial roles in the post- transcriptional regulation of gene expression, including modulation of the transport of mRNAs out of the nucleus and of translation efficiency, subcellular localization and stability. The molecu-lar profiling of many diseases has shown that the untranslated regions of mRNAs play a more significant role in disease progression and susceptibility than previously thought. Therefore, the methods used in the mapping and detection of these regions continue to gain importance. De-pending on technology development, each test method makes significant progress through uni-versity research and commercial companies. Therefore, it provides more detailed information about the mutations that occur in these regions and their effects on the formation of the disease. In this study, the tests used to detect untranslated regions are described. These tests include Pri-mer Extension Assay, S1 Nuclease Assay,RNAse Protection Assay, and Rapid Amplification of cDNA Ends Assay. These assays can be used to map the 5’ and 3’ terminus of an RNA and to quantitate the amount of a given RNA by extending a primer using reverse transcriptase.

Keywords

Protein Kodlanmayan Bölgelerin (UTR) Rolü ve Bu Bölgelerin Haritalanmasında Kullanılan Başlıca Yöntemler

Öz

Virüs genomlarının çevrilmemiş bölgelerinin (UTR'ler), mRNA'ların çekirdek dışına taşınması ve translasyon verimliliği dahil olmak üzere gen ifadesinin transkripsiyon sonrası düzenlenme-sinde önemli roller oynadığı bilinmektedir. Birçok hastalığın moleküler profilinin çıkarılması, mRNA'ların çevrilmemiş bölgelerinin hastalığın ilerlemesinde ve yatkınlığında düşünülenden daha önemli bir rol oynadığını göstermiştir. Bu nedenle, bu bölgelerin haritalanması ve tespitinde kullanılan yöntemler önem kazanmaya devam etmektedir. Dolayısıyla bu bölgelerde meydana gelen mutasyonlar ve bunların hastalık oluşumuna etkileri hakkında daha detaylı bilgi edinilmesi gerekmektedir. Bu çalışmada, çevrilmemiş bölgeleri tespit etmek için kullanılan testler anlatıl-maktadır. Teknoloji gelişimine bağlı olarak, her test yöntemi üniversite araştırmaları ve ticari şir-ketler aracılığıyla önemli ilerlemeler kaydetmektedir. Bu testler Primer Extension Assay, S1 Nuc-lease Assay, RNAse Protection Assay ve Rapid Amplification of cDNA Ends Assay'i içermek-tedir. Bu testler, bir RNA'nın 5' ve 3' terminalini haritalamak ve ters transkriptaz kullanarak bir primeri uzatarak belirli bir RNA miktarını ölçmek için kullanılmaktadır.

Anahtar Kelimeler

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Details

Primary Language

Turkish

Subjects

Veterinary Virology

Journal Section

Review

Authors

Seda Gözel ^*
0000-0003-2976-5245
Türkiye

Cüneyt Tamer
0000-0003-3240-8425
Türkiye

Early Pub Date

June 30, 2024

Publication Date

June 30, 2024

Submission Date

January 10, 2024

Acceptance Date

February 26, 2024

Published in Issue

Year 2024 Volume: 6 Number: 1

DOI

https://doi.org/10.51755/turkvetj.1417084

IZ

https://izlik.org/JA43TW68SA

Cite

RIS / Bibtex

APA

Gözel, S., & Tamer, C. (2024). Protein Kodlanmayan Bölgelerin (UTR) Rolü ve Bu Bölgelerin Haritalanmasında Kullanılan Başlıca Yöntemler. Turkish Veterinary Journal, 6(1), 12-17. https://doi.org/10.51755/turkvetj.1417084