The Effects of Tribulus Terrestris on Aquaporin-1 (AQP1) Immunolocalization in Small and Large Intestines of Mice

Şükran Aras; Gökhan Bayrakcı; Ebru Karadağ Sarı

doi:10.51755/turkvetj.1106914

TR EN

Tribulus Terrestris’in Fare İnce ve Kalın Bağırsaklarında Aquaporin-1 (AQP1) Salınımı Üzerine Etkisi

Öz

Çalışmada Demir Dikeni’nin (Tribulus Terrestris) fare ince ve kalın bağırsaklarında Aquaporin-1 (AQP1) immunolokalizasyonu üzerine etkisinin incelenmesi amaçlandı. Çalışma için 16 adet BALB/c cinsi erkek fare kullanıldı. Kontrol ve deney grupları; her bir grupta 8 hayvan olacak şekilde rastgele seçilen farelerden oluşturuldu. Çalışma sonunda alınan bağırsak dokularına rutin histolojik ve immunohistokimyasal yöntemler uygulandı. Yapılan değerlendirmelerde tüm grupların bağırsak dokularının normal histolojide olduğu, kontrol grubu ile demir dikeni (DD) grubu arasında goblet hücresi sayısı bakımından ileum, sekum, kolon ve rektumda istatistiksel olarak anlamlı bir fark olduğu belirlendi. İki grupta da ince ve kalın bağırsaklarda AQP1immunoreaktivitesi belirlendi. DD grubunda villus intestinalisler ve kript epitel hücrelerinin apikal sitoplazmasında AQP1 immunoreaktivitesinin arttığı tespit edildi. Sonuç olarak DD uygulamasının AQP1 immunoreaktivitesini arttırdığı ve buna bağlı olarak bağırsaklarda meydana gelen sindirim emilim olaylarında rolleri olabileceği düşünüldü.

Anahtar Kelimeler

The Effects of Tribulus Terrestris on Aquaporin-1 (AQP1) Immunolocalization in Small and Large Intestines of Mice

Abstract

This study aimed to examine the effects of Tribulus Terrestris on Aquaporin-1 (AQP1) immunolocalization in the small and large intestines of mice. A total of 16 male BALB/c mice were used in the study. The control and experiment groups consisted of randomly selected mice, with each group containing eight animals. Small and large intestine tissues of mice were taken under deep anesthesia at the end of the study. Routine histological and immunohistochemical methods were applied on the intestinal tissues obtained at the end of the study. The analyses indicated that the intestinal tissues of all groups had a normal histology, and there was a statistically significant difference between the numbers of goblet cells of the control and Tribulus Terrestris (TT) groups in the ileum, cecum, colon and rectum. AQP1immunoreactivity was detected in the small and large intestines of both groups. AQP1immunoreactivity increased in the apical cytoplasm of the intestinal villus and crypt epithelium cells in the small intestine of the TT group. Application of TT increased AQP1immunoreactivity and might have a role in absorption activities in the intestines.

Keywords

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

Veteriner Cerrahi

Bölüm

Araştırma Makalesi

Yazarlar

Şükran Aras ^*
0000-0002-3267-5251
Türkiye

Gökhan Bayrakcı
0000-0002-1193-658X
Türkiye

Ebru Karadağ Sarı
0000-0001-7581-6109
Türkiye

Yayımlanma Tarihi

23 Aralık 2022

Gönderilme Tarihi

21 Nisan 2022

Kabul Tarihi

27 Haziran 2022

Yayımlandığı Sayı

Yıl 2022 Cilt: 4 Sayı: 1

DOI

https://doi.org/10.51755/turkvetj.1106914

IZ

https://izlik.org/JA49EP77WS

Kaynak Göster

RIS / Bibtex

APA

Aras, Ş., Bayrakcı, G., & Karadağ Sarı, E. (2022). The Effects of Tribulus Terrestris on Aquaporin-1 (AQP1) Immunolocalization in Small and Large Intestines of Mice. Turkish Veterinary Journal, 4(1), 10-17. https://doi.org/10.51755/turkvetj.1106914

Cited By

LOCALIZATION OF AQUAPORIN-1 IN THE SMALL AND LARGE INTESTINES OF GEESE (Anser anser)

Slovenian Veterinary Research

https://doi.org/10.26873/SVR-1711-2023