The Effects of Tribulus Terrestris on Aquaporin-1 (AQP1) Immunolocalization in Small and Large Intestines of Mice

Şükran Aras; Gökhan Bayrakcı; Ebru Karadağ Sarı

doi:10.51755/turkvetj.1106914

TR EN

Tribulus Terrestris’in Fare İnce ve Kalın Bağırsaklarında Aquaporin-1 (AQP1) Salınımı Üzerine Etkisi

Öz

Çalışmada Demir Dikeni’nin (Tribulus Terrestris) fare ince ve kalın bağırsaklarında Aquaporin-1 (AQP1) immunolokalizasyonu üzerine etkisinin incelenmesi amaçlandı. Çalışma için 16 adet BALB/c cinsi erkek fare kullanıldı. Kontrol ve deney grupları; her bir grupta 8 hayvan olacak şekilde rastgele seçilen farelerden oluşturuldu. Çalışma sonunda alınan bağırsak dokularına rutin histolojik ve immunohistokimyasal yöntemler uygulandı. Yapılan değerlendirmelerde tüm grupların bağırsak dokularının normal histolojide olduğu, kontrol grubu ile demir dikeni (DD) grubu arasında goblet hücresi sayısı bakımından ileum, sekum, kolon ve rektumda istatistiksel olarak anlamlı bir fark olduğu belirlendi. İki grupta da ince ve kalın bağırsaklarda AQP1immunoreaktivitesi belirlendi. DD grubunda villus intestinalisler ve kript epitel hücrelerinin apikal sitoplazmasında AQP1 immunoreaktivitesinin arttığı tespit edildi. Sonuç olarak DD uygulamasının AQP1 immunoreaktivitesini arttırdığı ve buna bağlı olarak bağırsaklarda meydana gelen sindirim emilim olaylarında rolleri olabileceği düşünüldü.

Anahtar Kelimeler

The Effects of Tribulus Terrestris on Aquaporin-1 (AQP1) Immunolocalization in Small and Large Intestines of Mice

Abstract

This study aimed to examine the effects of Tribulus Terrestris on Aquaporin-1 (AQP1) immunolocalization in the small and large intestines of mice. A total of 16 male BALB/c mice were used in the study. The control and experiment groups consisted of randomly selected mice, with each group containing eight animals. Small and large intestine tissues of mice were taken under deep anesthesia at the end of the study. Routine histological and immunohistochemical methods were applied on the intestinal tissues obtained at the end of the study. The analyses indicated that the intestinal tissues of all groups had a normal histology, and there was a statistically significant difference between the numbers of goblet cells of the control and Tribulus Terrestris (TT) groups in the ileum, cecum, colon and rectum. AQP1immunoreactivity was detected in the small and large intestines of both groups. AQP1immunoreactivity increased in the apical cytoplasm of the intestinal villus and crypt epithelium cells in the small intestine of the TT group. Application of TT increased AQP1immunoreactivity and might have a role in absorption activities in the intestines.

Keywords

References

Akbulut G, Çiftçi H, Yıldız E (2008) Sindirim sistemi hastalıkları ve beslenme tedavisi.Access:http://beslenme.gov.tr/content/files/yeterlibeslenme/hastaliklarda_beslenme/c8.pdf.
Aktümsek A (2020) Anatomi ve fizyoloji. Nobel Yayın Dağıtım, Ankara.
Arcasoy HB, Erenmemişoğlu A, Tekol Y, Kurucu S, Kartal M (1998) Effect of Tribulus Terrestris L. saponin mixture on some smooth muscle preparations: A preliminary study. Bollettino Chimico Farmaceutico 137: 473-475. PMID: 10077881.
Arciszewski MB, Stefaniak M, Zacharko-Siembida A, Całka J (2011) Aquaporin 1 water channel is expressed on submucosal but not myenteric neurons from the ovine duodenum. Annals of Anatomy 193: 81-85. doi: 10.1016/j.aanat.2010.11.003
Ayyanna C, Chandra Mohan Rao G, Sasikala M, Somasekhar P (2012) Absorption enhancement studies of metformin hydrochloride by using tribulus terrestris plant extract. International Journal of Pharmacy and Technology 4: 4118-4125.
Brown D, Verbavatz JM, Valenti G, Lui B, Sabolic I (1993) Localization of the CHIP28 water channel in reabsorptive segments of the rat male reproductive tract. European Journal of Cell Biology 61: 264-273. PMID: 8223717.
Brown DC, Maxwell CV, Erf GF, Davis ME, Singh S, Johnson ZB (2006) The influence of different management systems and age on intestinal morphology, immune cell numbers and mucin production from goblet cells in post weaning pigs. Veterinary Immunology and Immunopathology 111 (3-4): 187-198. doi:10.1016/j.vetimm.2005.12.006.
Calamita G, Ferri D, Bazzini C, Mazzone A, Botta G, Liquori GE, Paulmichl M, Portincasa P, Meyer G, Svelto M (2005) Expression and subcellular localization of the AQP8 and AQP1 waterchannels in the mouse gall-bladder epithelia cells. Biology of the Cell 97: 415-423. doi: 10.1042/BC20040137.

Cao M, Yang M, Ou Z, Li D, Geng L, Chen P, Chen H, Gong S (2014) Involvement of aquaporins in a mouse model of rotavirus diarrhea. Virologica Sinica 29: 211-217. doi: 10.1007/s12250-014-3469-z.
De Luca A, Vassalotti G, Pelagalli A, Pero ME, Squillacioti C, Mirabella N, Lombardi P, Avallone L (2015) Expression and localization of aquaporin-1 along the intestine of colostrum suckling buffalo calves. Anatomia Histologia Embryologia 44: 391-400. doi: 10.1111/ahe.12157.
Deplancke B, Gaskins HR (2001) Microbial modulation of innate defense: goblet cells and the intestinal mucus layer. The American Journal of Clinical Nutrition 73: 1131-1141. doi: 10.1093/ajcn/73.6.1131S.
Gao J, Zhang HJ, Yu SH, Wu SG, Yoon I, Quigley J, Qi GH (2008) Effects of yeast culture in broiler diets on performance and immunomodulatory functions. Poultry Science 87: 1377-1384. doi: 10.3382/ps.2007-00418.
Gersemann M, Becker S, Kübler I, Koslowski M, Wang G, Herrlinger KR, Griger J, Fritz P, Fellermann K, Schwab M, Wehkamp J, Stange EF (2009) Differences in goblet cell differentiation between Crohn's disease and ulcerative colitis. Differentiation 77 (1): 84-94. doi: 10.1016/j.diff.2008.09.008.
Hamedi S, Rezaian M, Shomali T (2011) Histological changes of small intestinal mucosa of cocks due to sunflower meal single feeding. American Journal of Animal and Veterinary Sciences 6 (4): 171-175. doi: 10.4081/ijas.2013.e7.
Higa K, Ochiai H, Fujise H (2000) Molecular cloning and expression of aquaporin 1 (AQP1) in dog kidney and erythroblasts. Biochimica et Biophysica Acta 1463: 374-382. doi: 10.1016/s0005-2736(99)00218-7.
Huang HF, He RH, Sun CC, Zhang Y, Meng QX, Ma YY (2006) Function of aquaporins in female and male reproductive systems. Human Reproduction 12: 785-795. doi: from:10.1093/humupd/dml035.
Karam SM (1999) Lineage commitment and maturation of epithelial cells in the gut. Frontiers in Bioscience 4: 286-298. doi: 10.2741/karam
King LS, Choi M, Fernandez PC, Cartron JP, Agre P (2001) Defective urinary-concentrating ability due to a complete deficiency of aquaporin-1. The New England Journal of Medicine 345: 175-179. doi: 10.1056/NEJM200107193450304.
Kostova I, Dinchev D, Rentsch GH, Dimitrov V, Ivanova A (2002) Two new sulfated furostanol saponins from Tribulus terrestris. Zeitschrift für Naturforschung C 57: 33-38. doi: 10.1515/znc-2002-1-206.
Koyama Y, Yamamoto T, Tani T, Nihei K, Kondo D, Funaki H, Yaoita E, Kawasaki K, Sato N, Hatakeyama K, Kihara I (1999) Expression and localization of aquaporins in rat gastrointestinal tract. American Journal of Physiology 276: 621-627. doi: 10.1152/ajpcell.1999.276.3.C621.
Laforenza U (2012) Water channel proteins in the gastrointestinal tract. Molecular Aspects of Medicine 33: 642-650. doi: 10.1016/j.mam.2012.03.001.
Laforenza U, Bottino C, Gastaldi G (2016) Mammalian aquaglyceroporin function in metabolism. Biochimica et Biophysica Acta 1858: 1-11. doi: 10.1016/j.bbamem.2015.10.004.
Ma T, Jayaraman S, Wang KS, Song Y, Yang B, Li J, Bastidas JA, Verkman AS (2001) Defective dietary fat processing in transgenic mice lacking aquaporin-1 water channels. American Journal of Physiology-Cell Physiology 280 (1): 126-134. doi: 10.1152/ajpcell.2001.280.1.C126.
Matsuzaki T, Tajika Y, Ablimit A, Aoki T, Hagiwara H, Takata K (2004) Aquaporins in the digestive system. Medical Electron Microscopy 37: 71-80. doi: 10.1007/s00795-004-0246-3.
Miller HR, Huntley JF, Wallace GR (1981) Immune exclusion and mucus trapping during the rapid expulsion of Nippostrongylus brasiliensis from primed rats. Immunology 44 (2): 419-429. PMCID: PMC1555212.
Mobasheri A, Marples D (2004) Expression of the AQP-1 water channel in normal human tissues:A semiquantitative study using tissue microarray technology. American Journal of Physiology-Cell Physiology 286: 529-537. doi: 10.1152/ajpcell.00408.2003.
Nielsen S, Smith BL, Christensen EI, Agre P (1993) Distribution of the aquaporin CHIP in secretory and resorptive epithelia and capillary endothelia. Proceedings of the National Academy of Sciences 90: 7275-7279. doi: 10.1073/pnas.90.15.7275.
Nourmohammadi R, Afzali N (2013) Effect of citric acid and microbial phytase on small intestinal morphology in broiler chicken. Italian Journal of Animal Science 12 (7): 44-47. doi: 10.4081/ijas.2013.e7.
Preston GM, Carroll TP, Guggino WB, Agre P (1992) Appearance of water channels in xenopus oocytes expression red cell CHIP28 protein. Science 56: 385-387. doi: 10.1126/science.256.5055.385.
Saadoun S, Papadopoulos MC, Hara-Chikuma M, Verkman AS (2005) Impairment of angiogenesis and cell migration by targeted aquaporin-1 gene disruption. Nature 434: 786-792. doi: 10.1038/nature03460.
Specian RD, Oliver MG (1991) Functional biology of intestinal goblet cells. American Journal of Physiology 260: 183-193. doi: 10.1152/ajpcell.1991.260.2.C183
Sui H, Han BJ, Lee JK, Walian P, Jap BK (2001) Structural basis of water-specific transport through the AQP1 water channel. Nature 414: 872-878. doi: 10.1038/414872a.
Tilwari A, Shukla NP, Devi U (2011) Effect of five medicinal plants used in Indian system of medicines on immune function in wistar rats. African Journal of Biotechnology 10: 16637-16645. doi: 10.5897/AJB10.2168.
Van Klinken BJW, Einerhand AWC, Duits LA, Makkink MK, Tytgat KMAJ, Renes IB, Verburg M, Büller AH, Dekker J (1999) Gastrointestinal expression and partial cDNA cloning of murine Muc2. American Journal of Physiology-Gastrointestinal and Liver Physiology 276: 115-124. doi: 10.1152/ajpgi.1999.276.1.G115.
Verkman AS (1998) Role of aquaporin water channels in kidney and lung. The American Journal of the Medical Sciences 316: 310-320. doi: 10.1097/00000441-199811000-00004.
Wintour EM (1997) Water channels and urea transporters. Clinical and Experimental Pharmacology and Physiology 24: 1-9. doi: 10.1111/j.1440-1681.1997.tb01775.x
Wu G, Jiang S, Jiang F, Zhu D, Wu H, Jiang S (1996) Steroidal glycosides from Tribulus Terrestris. Phytochemistry 42: 1677–1681. doi: 10.1016/0031-9422(96)00182-3.
Yunus M, Horii Y, Makimura S, Smith AL (2005) Murine goblet cell hypoplasia during Eimeria pragensis infection is ameliorated by clindamycin treatment. Journal Veterinary Medical Science 67 (3): 311-315. doi: 10.1292/jvms.67.311.
Zhang SJ, Qu WJ, Zhong SY (2006) Inhibitory Effects of saponins from Tribulus Terrestris on alpha-glucosidase in small intestines of rats. Zhongguo Zhong Yao Za Zhi 31(11): 910-13. PMID: 17048632.

Details

Primary Language

English

Subjects

Veterinary Surgery

Journal Section

Research Article

Authors

Şükran Aras ^*
0000-0002-3267-5251
Türkiye

Gökhan Bayrakcı
0000-0002-1193-658X
Türkiye

Ebru Karadağ Sarı
0000-0001-7581-6109
Türkiye

Publication Date

December 23, 2022

Submission Date

April 21, 2022

Acceptance Date

June 27, 2022

Published in Issue

Year 2022 Volume: 4 Number: 1

DOI

https://doi.org/10.51755/turkvetj.1106914

IZ

https://izlik.org/JA49EP77WS

Cite

RIS / Bibtex

APA

Aras, Ş., Bayrakcı, G., & Karadağ Sarı, E. (2022). The Effects of Tribulus Terrestris on Aquaporin-1 (AQP1) Immunolocalization in Small and Large Intestines of Mice. Turkish Veterinary Journal, 4(1), 10-17. https://doi.org/10.51755/turkvetj.1106914

Cited By

LOCALIZATION OF AQUAPORIN-1 IN THE SMALL AND LARGE INTESTINES OF GEESE (Anser anser)

Slovenian Veterinary Research

https://doi.org/10.26873/SVR-1711-2023