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Investigation of M. alkalescens, M. arginini, M.dispar, M. canis and M. bovirhinis on cattle pneumonia cases observed in Sivas region

Year 2019, Volume: 1 Issue: 1, 10 - 15, 19.04.2019

Mahmut Moğulkoç , Murat Yıldırım

Abstract

M. bovis is isolated frequently among bacterial agents in cattle pneumonia cases. However, there hasn’t been any information about the presence of M. alkalescens, M. arginini, M.dispar, M. canis and M. bovirhinis agents outside of M. bovis in Sivas region. In the current study, it was aimed to detect other mycoplasma agents except M. bovis in cattle pneumonia cases. With this aim, slaughtered cattle lungs were examined in terms of pneumonia in Sivas city and a total of 200 pneumonic lungs were evaluated for mycoplasma agents by culture method. Mycoplasma suspected cultures were examined with PCR and M. alkalescens, M. arginini, M.dispar and M. bovirhinis detection rates were 2.5%, 1%, 4% and 2%, respectively. As a consequence, these mycoplasma species should be taken into consideration in bovine pneumonia cases.

Keywords

Mycoplasma PCR cattle pneumonia

References

  • Almeida RA, Rosenbusch RF (1991) Capsulelike surface material of Mycoplasma dispar induced by in vitro growth in culture with bovine cells is antigenically related to similar structures expressed in vivo. Infect Immun 59(9), 3119-3125.
  • Ayling RD, Bashiruddin SE, Nicholas RAJ (2004) Mycoplasma species and related organisms isolated from ruminants in Britain between 1990 and 2000. Vet Rec 155(14): 413-416. http://dx.doi.org/10.1136/vr.155.14.413
  • Blackburn P, McAuliffe L, Nicholas RAJ, Ball HJ (2010) Comparison of methods for detecting mycoplasmas in calf pneumonia cases. The Vet Rec 166(18): 561. http://dx.doi.org/10.1136/vr.b4833
  • Chazel M, Tardy F, Grand D, Calavas D, Poumarat F (2010) Mycoplasmoses of ruminants in France: recent data from the national surveillance network. BMC Vet Res 6(1): 32. https://doi.org/10.1186/1746-6148-6-32
  • Jasper DE (1981) Bovine mycoplasmal mastitis. Adv Vet Sci Comp Med 25: 121-57
  • Kobayashı H, Hırose K, Worarach A, Paugtes P, Ito N, Morozumı T, Yamamoto K (1998) In vitro amplification of the 16S rRNA genes from Mycoplasma bovirhinis, Mycoplasma alkalescens and Mycoplasma bovigenitalium by PCR. J Vet Sci 60 (12): 1299-1303. https://doi.org/ 10.1292/jvms.60.1299
  • Kokotovic B, Friis NF, Ahrens P (2007) Mycoplasma alkalescens demonstrated in bronchoalveolar lavage of cattle in Denmark. Acta Vet Scand, 49(1): 2. https://dx.doi.org/10.1186%2F1751-0147-49-2
  • Komoda M, Kozai Y, Itoi H, Noro A, Yamada T, Kimura Y, Koizumi S (1988). Outbreak of shipping fever associated with several pathogens in grazing calves. JMVA 41(6): 408-411.
  • Lawes JR, Bisgaard-frantzen S, Bashiruddin SE, McAuliffe L, Ayling RD, Nicholas RAJ (2006) Emergence of M. alkalescens in cattle in the UK. 16th international congress International Organisation for Mycoplasmology. Cambridge, UK. 2006. pp. 129.
  • Lysnyansky I, Levisohn S, Perl S, Malone F, Ball HJ (2008) Immunohistochemical detection of Mycoplasma alkalescens in naturally infected calves. Proceedings of the 17th International Congress of the International Organisation for Mycoplasmology, Tianjin, China.
  • Marques LM, Buzinhani M, Yamaguti M, Oliveira RC, Ferreira JB, Mettifogo E, Timenetsky J (2007) Use of a polymerase chain reaction for detection of Mycoplasma dispar in the nasal mucus of calves. J Vet Diagn Invest 19(1): 103-106. https://doi.org/10.1177/104063870701900118
  • McAuliffe L, Ellis RJ, Lawes JR, Ayling RD, Nicholas RA (2005) 16S rDNA PCR and denaturing gradient gel electrophoresis; a single generic test for detecting and differentiating Mycoplasma species. J Med Microbiol 54:731-739. https://doi.org/10.1099/jmm.0.46058-0
  • Miles K, McAuliffe L, Ayling RD, Nicholas RA (2004) Rapid detection of Mycoplasma dispar and M. bovirhinis using allele specific polymerase chain reaction protocols. FEMS Microbiol Lett 241(1): 103-107. https://doi.org/10.1016/j.femsle.2004.10.010
  • Nicholas RA, Hannam DA, Baker SE, Weaver CR (1995) Mycoplasma canis in a British calf. The Vet Rec, 137(17): 443-444. http://dx.doi.org/10.1136/vr.137.17.443Nicholas R, Baker S, Ayling R, Stipkovits L (2000) Mycoplasma infections in growing cattle. Cattle Pract, 8(2): 115-118.
  • Nicholas RA, Ayling RD, Stipkovits LP (2002) An experimental vaccine for calf pneumonia caused by Mycoplasma bovis: clinical, cultural, serological and pathological findings. Vaccine 20(29-30), 3569-3575. https://doi.org/10.1016/S0264-410X(02)00340-7
  • Nicholas R, Ayling R, McAuliffe L (2008) Mycoplasma diseases of ruminants. CABI
  • Ozen H, Karaman M, Sahin M, Ozcan K (2009) Pnömonili Sığırlarda Mycoplasma bovis, M. dispar, M. bovirhinis ve M. mycoides subsp. mycoides (küçük koloni tipi)’in PZR ile belirlenerek patolojik bulguların incelenmesi. Kafkas Univ Vet Fak Derg 15(1): 125-133. https://doi.org/10.9775/kvfd.2008.108-A
  • Ross, RF (1993) Mycoplasma-animal pathogens. In Rapid diagnosis of mycoplasmas (pp. 69-109). Springer, Boston, MA.
  • Sambrook J, Russell DW (2006) Purification of nucleic acids by extraction with phenol:chloroform. CSH Protoc 356-359. https://doi.org/10.1101/pdb.prot093450
  • Sayin Z, Sakmanoğlu A, Ucan US, Uslu A, Hadimli HH, Aras Z, Erganiş O (2016) Mycoplasma infections in dairy cattle farms in Turkey. Turk J Vet Anim Sci 40(5): 569-574. https://doi.org/10.3906/vet-1602-82
  • Tegtmeier C, Uttenthal AA, Friis NF, Jensen NE, Jensen HE (1999) Pathological and microbiological studies on pneumonic lungs from Danish calves. J Vet Med Series B, 46(10): 693-700. https://doi.org/10.1046/j.1439-0450.1999.00301.x
  • Timenetsky J, Santos LM, Buzinhani M, Mettifogo E (2006) Detection of multiple mycoplasma infection in cell cultures by PCR. Braz J Med Bıol Res 39(7), 907-914. https://doi.org/10.1590/S0100-879X2006000700009
  • Thomas A, Dizier I, Trolin A, Mainil J, Linden A, Ball H, Bell C (2002) Isolation of mycoplasma species from the lower respiratory tract of healthy cattle and cattle with respiratory disease in Belgium. Vet Rec, 151(16): 472-476. https://dx.doi.org/10.1136/vr.151.16.472
  • Virtala AM, Mechor GD, Gröhn YT, Erb HN, Dubovi EJ (1996) Epidemiologic and pathologic characteristics of respiratory tract disease in dairy heifers during the first three months of life. J Am Vet Med Assoc 208(12): 2035-2042.
  • Wong-Lee J G, & Lovett M (1993) Diagnostic Molecular and Microbiology Principles and Applications. American Society for Microbiology, Washington DC

Sivas Yöresinde Gözlenen Sığır Pnömoni Vakalarında M. alkalescens, M. arginini, M.dispar, M. canis ve M. bovirhinis Varlığının Araştırılması

Year 2019, Volume: 1 Issue: 1, 10 - 15, 19.04.2019

Mahmut Moğulkoç , Murat Yıldırım

Abstract

Sığır pnömoni vakalarında M. bovis bakteriyel etkenler arasında sıklıkla teşhis edilmektedir. Ancak Sivas bölgesinde M. bovis dışında M. alkalescens, M. arginini, M.dispar, M. canis ve M. bovirhinis’in varlığı hakkında bilgiye rastlanılmamıştır.  Bu çalışmada Sivas bölgesindeki sığır pnömoni vakalarında M. bovis haricindeki diğer mikoplazma etkenlerinin tespit edilmesi amaçlanmıştır. Bu amaçla Sivas il merkezi mezbahalarında kesimi yapılmış sığırların akciğerleri pnömoni bulgularına göre incelenmiş ve toplamda 200 adet pnömonik akciğer örneği laboratuvarda kültürü yapılarak mikoplazma etkenleri yönünden değerlendirilmiştir. Mikoplazma şüpheli kültürler polimeraz zincir reaksiyonu (PZR) ile incelendiğinde örneklerin %2.5’u M. alkalescens, %0.5’i M. arginini, %2’si M. dispar ve %1’i M. bovirhinis pozitif bulundu. M. canis ise tespit edilemedi. Sonuç olarak sığır pnömoni vakalarında bu mikoplazma türleri de teşhiste dikkate alınmalıdır.

Keywords

Mikoplazma PZR pnömoni sığır pnömoni

References

  • Almeida RA, Rosenbusch RF (1991) Capsulelike surface material of Mycoplasma dispar induced by in vitro growth in culture with bovine cells is antigenically related to similar structures expressed in vivo. Infect Immun 59(9), 3119-3125.
  • Ayling RD, Bashiruddin SE, Nicholas RAJ (2004) Mycoplasma species and related organisms isolated from ruminants in Britain between 1990 and 2000. Vet Rec 155(14): 413-416. http://dx.doi.org/10.1136/vr.155.14.413
  • Blackburn P, McAuliffe L, Nicholas RAJ, Ball HJ (2010) Comparison of methods for detecting mycoplasmas in calf pneumonia cases. The Vet Rec 166(18): 561. http://dx.doi.org/10.1136/vr.b4833
  • Chazel M, Tardy F, Grand D, Calavas D, Poumarat F (2010) Mycoplasmoses of ruminants in France: recent data from the national surveillance network. BMC Vet Res 6(1): 32. https://doi.org/10.1186/1746-6148-6-32
  • Jasper DE (1981) Bovine mycoplasmal mastitis. Adv Vet Sci Comp Med 25: 121-57
  • Kobayashı H, Hırose K, Worarach A, Paugtes P, Ito N, Morozumı T, Yamamoto K (1998) In vitro amplification of the 16S rRNA genes from Mycoplasma bovirhinis, Mycoplasma alkalescens and Mycoplasma bovigenitalium by PCR. J Vet Sci 60 (12): 1299-1303. https://doi.org/ 10.1292/jvms.60.1299
  • Kokotovic B, Friis NF, Ahrens P (2007) Mycoplasma alkalescens demonstrated in bronchoalveolar lavage of cattle in Denmark. Acta Vet Scand, 49(1): 2. https://dx.doi.org/10.1186%2F1751-0147-49-2
  • Komoda M, Kozai Y, Itoi H, Noro A, Yamada T, Kimura Y, Koizumi S (1988). Outbreak of shipping fever associated with several pathogens in grazing calves. JMVA 41(6): 408-411.
  • Lawes JR, Bisgaard-frantzen S, Bashiruddin SE, McAuliffe L, Ayling RD, Nicholas RAJ (2006) Emergence of M. alkalescens in cattle in the UK. 16th international congress International Organisation for Mycoplasmology. Cambridge, UK. 2006. pp. 129.
  • Lysnyansky I, Levisohn S, Perl S, Malone F, Ball HJ (2008) Immunohistochemical detection of Mycoplasma alkalescens in naturally infected calves. Proceedings of the 17th International Congress of the International Organisation for Mycoplasmology, Tianjin, China.
  • Marques LM, Buzinhani M, Yamaguti M, Oliveira RC, Ferreira JB, Mettifogo E, Timenetsky J (2007) Use of a polymerase chain reaction for detection of Mycoplasma dispar in the nasal mucus of calves. J Vet Diagn Invest 19(1): 103-106. https://doi.org/10.1177/104063870701900118
  • McAuliffe L, Ellis RJ, Lawes JR, Ayling RD, Nicholas RA (2005) 16S rDNA PCR and denaturing gradient gel electrophoresis; a single generic test for detecting and differentiating Mycoplasma species. J Med Microbiol 54:731-739. https://doi.org/10.1099/jmm.0.46058-0
  • Miles K, McAuliffe L, Ayling RD, Nicholas RA (2004) Rapid detection of Mycoplasma dispar and M. bovirhinis using allele specific polymerase chain reaction protocols. FEMS Microbiol Lett 241(1): 103-107. https://doi.org/10.1016/j.femsle.2004.10.010
  • Nicholas RA, Hannam DA, Baker SE, Weaver CR (1995) Mycoplasma canis in a British calf. The Vet Rec, 137(17): 443-444. http://dx.doi.org/10.1136/vr.137.17.443Nicholas R, Baker S, Ayling R, Stipkovits L (2000) Mycoplasma infections in growing cattle. Cattle Pract, 8(2): 115-118.
  • Nicholas RA, Ayling RD, Stipkovits LP (2002) An experimental vaccine for calf pneumonia caused by Mycoplasma bovis: clinical, cultural, serological and pathological findings. Vaccine 20(29-30), 3569-3575. https://doi.org/10.1016/S0264-410X(02)00340-7
  • Nicholas R, Ayling R, McAuliffe L (2008) Mycoplasma diseases of ruminants. CABI
  • Ozen H, Karaman M, Sahin M, Ozcan K (2009) Pnömonili Sığırlarda Mycoplasma bovis, M. dispar, M. bovirhinis ve M. mycoides subsp. mycoides (küçük koloni tipi)’in PZR ile belirlenerek patolojik bulguların incelenmesi. Kafkas Univ Vet Fak Derg 15(1): 125-133. https://doi.org/10.9775/kvfd.2008.108-A
  • Ross, RF (1993) Mycoplasma-animal pathogens. In Rapid diagnosis of mycoplasmas (pp. 69-109). Springer, Boston, MA.
  • Sambrook J, Russell DW (2006) Purification of nucleic acids by extraction with phenol:chloroform. CSH Protoc 356-359. https://doi.org/10.1101/pdb.prot093450
  • Sayin Z, Sakmanoğlu A, Ucan US, Uslu A, Hadimli HH, Aras Z, Erganiş O (2016) Mycoplasma infections in dairy cattle farms in Turkey. Turk J Vet Anim Sci 40(5): 569-574. https://doi.org/10.3906/vet-1602-82
  • Tegtmeier C, Uttenthal AA, Friis NF, Jensen NE, Jensen HE (1999) Pathological and microbiological studies on pneumonic lungs from Danish calves. J Vet Med Series B, 46(10): 693-700. https://doi.org/10.1046/j.1439-0450.1999.00301.x
  • Timenetsky J, Santos LM, Buzinhani M, Mettifogo E (2006) Detection of multiple mycoplasma infection in cell cultures by PCR. Braz J Med Bıol Res 39(7), 907-914. https://doi.org/10.1590/S0100-879X2006000700009
  • Thomas A, Dizier I, Trolin A, Mainil J, Linden A, Ball H, Bell C (2002) Isolation of mycoplasma species from the lower respiratory tract of healthy cattle and cattle with respiratory disease in Belgium. Vet Rec, 151(16): 472-476. https://dx.doi.org/10.1136/vr.151.16.472
  • Virtala AM, Mechor GD, Gröhn YT, Erb HN, Dubovi EJ (1996) Epidemiologic and pathologic characteristics of respiratory tract disease in dairy heifers during the first three months of life. J Am Vet Med Assoc 208(12): 2035-2042.
  • Wong-Lee J G, & Lovett M (1993) Diagnostic Molecular and Microbiology Principles and Applications. American Society for Microbiology, Washington DC
There are 25 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Mahmut Moğulkoç 0000-0003-3407-8132

Murat Yıldırım

Publication Date April 19, 2019
Published in Issue Year 2019 Volume: 1 Issue: 1

Cite

APA Moğulkoç, M., & Yıldırım, M. (2019). Sivas Yöresinde Gözlenen Sığır Pnömoni Vakalarında M. alkalescens, M. arginini, M.dispar, M. canis ve M. bovirhinis Varlığının Araştırılması. Turkish Veterinary Journal, 1(1), 10-15.
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