The Effect of Croton-Oil-Induced Experimental Hemorrhoid Model on Testicular Histology and Some Sperm Parameters

Volkan Koşal; Halil Cumhur Yılmaz

doi:10.51755/turkvetj.1713074

EN TR

The Effect of Croton-Oil-Induced Experimental Hemorrhoid Model on Testicular Histology and Some Sperm Parameters

Abstract

It was aimed to investigate the effects of Croton oil obtained from the Croton Tiglium plant on testicular histology and sperm parameters in male rats in an experimental hemorrhoid model caused by rectal administration. Sixteen male Albino Wistar rats were used for the experimental study. The animals were randomly divided into 2 groups. No treatment was applied to the control group (n=8). In the Croton-oil group (n:8), a hemorrhoid model was created by applying Croton-oil into the rectum with the help of a swab. The rats were sacrificed after 5 days. After sacrifice, testicular tissue samples were fixed with 10% neutral buffered formalin and Bouin's fixative solution for histologic examination. Motility, density, and abnormal sperm rates were determined in sperm samples obtained by puncture of the cauda epididymis. Histologically, it was observed that the lumens of the capillaries between the seminiferous tubules were enlarged in the Croton-oil-treated group, but there was no change in spermatogenic cells, Sertoli cells, and Leydig cells. In sperm analysis, no statistically significant difference was found in motility rate, density, and abnormal sperm rates (p>0.05). It was determined that local venous inflammation caused by Croton oil in the rectal area may have partial effects on the venous structures in the testis, but did not cause any change in sperm parameters.

Keywords

Kroton-Yağıyla Oluşturulan Deneysel Hemoroid Modelinin Testis Histolojisi ve Bazı Sperm Parametreleri Üzerine Etkisi

Abstract

Croton tiglium bitkisinden elde edilen kroton yağının rektal uygulama ile oluşturulan deneysel hemoroid modelinde erkek sıçanlarda testis histolojisi ve sperm parametreleri üzerine etkilerinin araştırılması amaçlanmıştır. Deneysel çalışma için 16 erkek Albino Wistar sıçan kullanıldı. Hayvanlar rastgele 2 gruba ayrılmıştır. Kontrol grubuna hiçbir tedavi uygulanmadı (n:8). Kroton yağı grubunda (n:8), kroton yağı rektum içine swap yardımıyla uygulanarak hemoroid modeli oluşturuldu. Sıçanlar 5 gün sonra sakrifiye edildi. Sakrifikasyondan sonra histolojik inceleme için testis doku örnekleri %10'luk nötral tamponlu formalin ve Bouin tespit solüsyonu ile tespit edildi. Kauda epididimis ponksiyonu ile elde edilen sperm örneklerinde motilite, yoğunluk ve anormal sperm oranları belirlendi. Histolojik olarak, Kroton yağı uygulanan grupta seminifer tübüller arasındaki kapillerlerin lümenlerinin genişlediği, ancak spermatojenik hücreler, Sertoli hücreleri ve Leydig hücrelerinde herhangi bir değişiklik olmadığı gözlendi. Sperm analizinde motilite oranı, yoğunluk ve anormal sperm oranlarında istatistiksel olarak anlamlı bir fark bulunmamıştır (p>0.05). Kroton yağının rektal bölgede neden olduğu lokal venöz inflamasyonun testisteki venöz yapılar üzerinde kısmi etkileri olabileceği, ancak sperm parametrelerinde herhangi bir değişikliğe neden olmadığı belirlendi.

Keywords

Ethical Statement

Animal experiments were performed at Van Yüzüncü Yil University Experimental Medicine Application and Research Centre with the approval of the local ethics committee (Decision no: 2025/05-10 Date:29/05/2025).

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Details

Primary Language

English

Subjects

Veterinary Histology and Embryology, Veterinary Urology

Journal Section

Research Article

Authors

Volkan Koşal ^*
0000-0001-5268-5046
Türkiye

Halil Cumhur Yılmaz
0000-0003-3958-7948
Türkiye

Early Pub Date

June 30, 2025

Publication Date

June 30, 2025

Submission Date

June 3, 2025

Acceptance Date

June 20, 2025

Published in Issue

Year 2025 Volume: 7 Number: 1

DOI

https://doi.org/10.51755/turkvetj.1713074

IZ

https://izlik.org/JA45PR99UY

Cite

RIS / Bibtex

APA

Koşal, V., & Yılmaz, H. C. (2025). The Effect of Croton-Oil-Induced Experimental Hemorrhoid Model on Testicular Histology and Some Sperm Parameters. Turkish Veterinary Journal, 7(1), 1-5. https://doi.org/10.51755/turkvetj.1713074

AMA

1.Koşal V, Yılmaz HC. The Effect of Croton-Oil-Induced Experimental Hemorrhoid Model on Testicular Histology and Some Sperm Parameters. Turk Vet J. 2025;7(1):1-5. doi:10.51755/turkvetj.1713074

Chicago

Koşal, Volkan, and Halil Cumhur Yılmaz. 2025. “The Effect of Croton-Oil-Induced Experimental Hemorrhoid Model on Testicular Histology and Some Sperm Parameters”. Turkish Veterinary Journal 7 (1): 1-5. https://doi.org/10.51755/turkvetj.1713074.

EndNote

Koşal V, Yılmaz HC (June 1, 2025) The Effect of Croton-Oil-Induced Experimental Hemorrhoid Model on Testicular Histology and Some Sperm Parameters. Turkish Veterinary Journal 7 1 1–5.

IEEE

[1]V. Koşal and H. C. Yılmaz, “The Effect of Croton-Oil-Induced Experimental Hemorrhoid Model on Testicular Histology and Some Sperm Parameters”, Turk Vet J, vol. 7, no. 1, pp. 1–5, June 2025, doi: 10.51755/turkvetj.1713074.

ISNAD

Koşal, Volkan - Yılmaz, Halil Cumhur. “The Effect of Croton-Oil-Induced Experimental Hemorrhoid Model on Testicular Histology and Some Sperm Parameters”. Turkish Veterinary Journal 7/1 (June 1, 2025): 1-5. https://doi.org/10.51755/turkvetj.1713074.

JAMA

1.Koşal V, Yılmaz HC. The Effect of Croton-Oil-Induced Experimental Hemorrhoid Model on Testicular Histology and Some Sperm Parameters. Turk Vet J. 2025;7:1–5.

MLA

Koşal, Volkan, and Halil Cumhur Yılmaz. “The Effect of Croton-Oil-Induced Experimental Hemorrhoid Model on Testicular Histology and Some Sperm Parameters”. Turkish Veterinary Journal, vol. 7, no. 1, June 2025, pp. 1-5, doi:10.51755/turkvetj.1713074.

Vancouver

1.Volkan Koşal, Halil Cumhur Yılmaz. The Effect of Croton-Oil-Induced Experimental Hemorrhoid Model on Testicular Histology and Some Sperm Parameters. Turk Vet J. 2025 Jun. 1;7(1):1-5. doi:10.51755/turkvetj.1713074