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The Biotechnological Potential of Baculoviruses: From Insect Viruses to Biotechnology Workhorse

Yıl 2025, Cilt: 7 Sayı: 1, 19 - 30

Remziye Özbek

https://doi.org/10.51755/turkvetj.1713887

Öz

Baculoviruses are a significant group of arthropod viruses that are widely recognized for their potential as biological control agents against pests in agriculture and forestry. The Baculoviridae is a vast family of viruses that primarily infects various species within the Arthropoda phylum, particularly insects. Baculoviruses are widely used not only as biopesticides in agricultural applications but also as efficient tools for recombinant protein production. The Baculovirus Expression Vector System (BEVS) has been shown to be particularly effective for expressing complex or difficult-to-produce proteins in mammalian cells. Owing to its high expression capacity and post-translational modification capabilities, BEVS has been successfully employed in various biotechnological fields, including vaccine development, therapeutic protein production, and the synthesis of enzymes and antibodies.
In this review, the BEVS technique, which is one of the significant areas of use of Baculoviruses, is discussed along with its advantages and several of its practical applications.

Anahtar Kelimeler

Baculovirus BEVS recombinant protein vaccine

Kaynakça

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The Biotechnological Potential of Baculoviruses: From Insect Viruses to Biotechnology Workhorse

Yıl 2025, Cilt: 7 Sayı: 1, 19 - 30

Remziye Özbek

https://doi.org/10.51755/turkvetj.1713887

Öz

Baculoviruses are a significant group of arthropod viruses that are widely recognized for their potential as biological control agents against pests in agriculture and forestry. The Baculoviridae is a vast family of viruses that primarily infects various species within the Arthropoda phylum, particularly insects. Baculoviruses are widely used not only as biopesticides in agricultural applications but also as efficient tools for recombinant protein production. The Baculovirus Expression Vector System (BEVS) has been shown to be particularly effective for expressing complex or difficult-to-produce proteins in mammalian cells. Owing to its high expression capacity and post-translational modification capabilities, BEVS has been successfully employed in various biotechnological fields, including vaccine development, therapeutic protein production, and the synthesis of enzymes and antibodies.
In this review, the BEVS technique, which is one of the significant areas of use of Baculoviruses, is discussed along with its advantages and several of its practical applications.

Anahtar Kelimeler

Baculovirus BEVS recombinant protein vaccine

Kaynakça

  • Adeniyi, A. A., & Lua, L. H. (2020). Protein Expression in the Baculovirus-Insect Cell Expression System. In: J. Gerrard, & L. Domigan (Eds.), Protein Nanotechnology: Methods in Molecular Biology (pp. 17–37). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9869-2_2
  • Aoki, H., Sakoda, Y., Jukuroki, K., Takada, A., Kida, H., & Fukusho, A. (1999). Induction of antibodies in mice by a recombinant baculovirus expressing pseudorabies virus glycoprotein B in mammalian cells. Veterinary microbiology, 68(3-4), 197–207. https://doi.org/10.1016/s0378-1135(99)00110-8
  • Balani, P., Boulaire, J., Zhao, Y., Zeng, J., Lin, J., & Wang, S. (2009). High mobility group box2 promoter-controlled suicide gene expression enables targeted glioblastoma treatment. Molecular therapy : the journal of the American Society of Gene Therapy, 17(6), 1003–1011. https://doi.org/10.1038/mt.2009.22
  • Blanco, J. C., Boukhvalova, M. S., Pletneva, L. M., Shirey, K. A., & Vogel, S. N. (2014). A recombinant anchorless respiratory syncytial virus (RSV) fusion (F) protein/monophosphoryl lipid A (MPL) vaccine protects against RSV-induced replication and lung pathology. Vaccine, 32(13), 1495–1500. https://doi.org/10.1016/j.vaccine.2013.11.032
  • Blazevic, V., Malm, M., Arinobu, D., Lappalainen, S., & Vesikari, T. (2016). Rotavirus capsid VP6 protein acts as an adjuvant in vivo for norovirus virus-like particles in a combination vaccine. Human vaccines & immunotherapeutics, 12(3), 740–748. https://doi.org/10.1080/21645515.2015.1099772
  • Bruder, M. R., & Aucoin, M. G. (2022). Utility of Alternative Promoters for Foreign Gene Expression Using the Baculovirus Expression Vector System. Viruses, 14(12), 2670. https://doi.org/10.3390/v14122670
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  • Liu, Y., Han, X., Qiao, Y., Wang, T., & Yao, L. (2023). Porcine Deltacoronavirus-like Particles Produced by a Single Recombinant Baculovirus Elicit Virus-Specific Immune Responses in Mice. Viruses, 15(5), 1095. https://doi.org/10.3390/v15051095
  • Luckow, V. A. (1993). Baculovirus systems for the expression of human gene products, Current Opinion in Biotechnology,4(5), 564-572. https://doi.org/10.1016/0958-1669(93)90078-B.
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  • Martínez-Solís, M., Gómez-Sebastián, S., Escribano, J. M., Jakubowska, A. K., & Herrero, S. (2016). A novel baculovirus-derived promoter with high activity in the baculovirus expression system. PeerJ, 4, e2183. https://doi.org/10.7717/peerj.2183
  • Metz, S. W., & Pijlman, G. P. (2011). Arbovirus vaccines; opportunities for the baculovirus-insect cell expression system. Journal of invertebrate pathology107 Suppl, S16–S30. https://doi.org/10.1016/j.jip.2011.05.002
  • Miao, Q., Nguyen, W., Zhu, J., Liu, G., van Oers, M. M., Tang, B., Yan, K., Larcher, T., Suhrbier, A., & Pijlman, G. P.(2024). A getah virus-like-particle vaccine providescomplete protection from viremia and arthritis in wild-type mice. Vaccine, 42(25), 126136.https://doi.org/10.1016/j.vaccine.2024.07.037
  • Miele, S. A., Garavaglia, M. J., Belaich, M. N., & Ghiringhelli, P. D. (2011). Baculovirus: molecular insights on their diversity and conservation. International journal of evolutionary biology, 2011, 379424. https://doi.org/10.4061/2011/379424
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  • Nguyen, H. T., Falzarano, D., Gerdts, V., & Liu, Q. (2024). Construction and immunogenicity of SARS-CoV-2 virus-like particle expressed by recombinant baculovirus BacMam. Microbiology spectrum, 12(8), e0095924. https://doi.org/10.1128/spectrum.00959-24
  • OECD (2023). Guidance document on Baculoviruses as plant protection products, Series on Pesticides and Biocides, OECD Publishing, Paris, https://doi.org/10.1787/8f0dc501-en.
  • Ono, C., Okamoto, T., Abe, T., & Matsuura, Y. (2018). Baculovirus as a Tool for Gene Delivery and Gene Therapy. Viruses, 10(9), 510. https://doi.org/10.3390/v10090510
  • Pidre, M. L., Arrías, P. N., Amorós Morales, L. C., & Romanowski, V. (2023). The Magic Staff: A Comprehensive Overview of Baculovirus-Based Technologies Applied to Human and Animal Health. Viruses, 15(1), 80. https://doi.org/10.3390/v15010080
  • Possee, R. D., Griffiths, C. M., Hitchman, R. B., Chambers, A., Murguia-Meca, F., Danquah, J., King, L. A. (2010). Baculoviruses: biology, replication and exploitation. In: Asgari, S and Johnson, K Insect Virology. Great Britain: Caister Academic Press. p35-58.
  • Puente-Massaguer, E., Lecina, M., & Gòdia, F. (2020). Application of advanced quantification techniques in nanoparticle-based vaccine development with the Sf9 cell baculovirus expression system. Vaccine, 38(7), 1849–1859. https://doi.org/10.1016/j.vaccine.2019.11.087
  • Razavi-Nikoo, H., Behboudi, E., Aghcheli, B., Hashemi, S. M.A., & Moradi, A. (2023). Bac to Bac SystemEfficiency for Preparing HPV Type 16 Virus-Like ParticleVaccine. Archives of Razi Institute, 78(3), 997–1003.https://doi.org/10.22092/ARI.2023.361975.2708
  • Rohrmann GF. Baculovirus Molecular Biology [Internet]. 4th edition. Bethesda (MD): National Center for Biotechnology Information (US); 2019. Chapter 9, Baculoviruses as insecticides: Four examples. Available from: https://www.ncbi.nlm.nih.gov/books/NBK543459/
  • Rychlowska, M., Gromadzka, B., Bieńkowska-Szewczyk, K., & Szewczyk, B. (2011). Application of baculovirus-insect cell expression system for human therapy. Current pharmaceutical biotechnology, 12(11), 1840–1849. https://doi.org/10.2174/138920111798377012
  • Saika, K., Kato, M., Sanada, H., Matsushita, S., Matsui, M., Handa, H., & Kawano, M. (2020). Induction of adaptive immune responses against antigens incorporated within the capsid of simian virus 40. The Journal of general virology, 101(8), 853–862. https://doi.org/10.1099/jgv.0.001445
  • Sandro, Q., Benchaouir, R. (2019) Baculovirus: A Powerful Tool for Various Biotechnological Applications. Advances in Biochemistry and Biotechnology, 7, 1085. DOI: 10.29011/2574-7258.001085
  • Sari, D., Gupta, K., Thimiri Govinda Raj, D. B., Aubert, A., Drncová, P., Garzoni, F., Fitzgerald, D., & Berger, I. (2016). The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics. Advances in experimental medicine and biology, 896, 199–215. https://doi.org/10.1007/978-3-319-27216-0_13
  • Schaly, S., Ghebretatios, M., & Prakash, S. (2021). Baculoviruses in Gene Therapy and Personalized Medicine. Biologics : targets & therapy, 15, 115–132. https://doi.org/10.2147/BTT.S292692
  • Skoberne, M., Cardin, R., Lee, A., Kazimirova, A., Zielinski, V., Garvie, D., Lundberg, A., Larson, S., Bravo, F. J., Bernstein, D. I., Flechtner, J. B., & Long, D. (2013). An adjuvanted herpes simplex virus 2 subunit vaccine elicits a T cell response in mice and is an effective therapeutic vaccine in Guinea pigs. Journal of virology, 87(7), 3930–3942. https://doi.org/10.1128/JVI.02745-12
  • Sokolenko, S., George, S., Wagner, A., Tuladhar, A., Andrich, J. M., & Aucoin, M. G. (2012). Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks. Biotechnology advances, 30(3), 766–781. https://doi.org/10.1016/j.biotechadv.2012.01.009
  • Tang, Y., Saul, J., Nagaratnam, N. ,Martin-Garcia , J. M., Fromme ,P., Qiu, J., & LaBaer, J. (2020). Construction of gateway-compatible baculovirus expression vectors for high-throughput protein expression and in vivo microcrystal screening. Scientific Report, 10, 13323. https://doi.org/10.1038/s41598-020-70163-2
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  • Wang, Z., Liu, M., Zhao, H., Wang, P., Ma, W., Zhang, Y., Wu, W., & Peng, C. (2021). Induction of Robust and Specific Humoral and Cellular Immune Responses by Bovine Viral Diarrhea Virus Virus-Like Particles (BVDV-VLPs) Engineered with Baculovirus Expression Vector System. Vaccines, 9(4), 350. https://doi.org/10.3390/vaccines9040350
  • Yang, Y. H., Tai, C. H., Cheng, D., Wang, Y. F., & Wang, J. R. (2022). Investigation of Avian Influenza H5N6 Virus-like Particles as a Broad-Spectrum Vaccine Candidate against H5Nx Viruses. Viruses, 14(5), 925. https://doi.org/10.3390/v14050925
  • Yin, X., Li, Z., Li, J., Yi, Y., Zhang, Y., Li, X., Li, B., Yang, B., Lan, X., Li, Y., Jiao, W., Zhang, Z., & Liu, J. (2013). Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice. Journal of biotechnology, 163(3), 333–338. https://doi.org/10.1016/j.jbiotec.2012.11.002
  • Yu, W., Li, J., Wang, M., Quan, Y., Chen, J., Nie, Z., Lv, Z.., & Zhang, Y. (2012). The screening and functional study of proteins binding with the BmNPV polyhedrin promoter. Virology Journal, 6( 90). https://doi.org/10.1186/1743-422X-9-90
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  • Zhang, Q., Sun, Y., Sun, Y., Zhang, H., & Yang, R. (2024). Expression of VP2 protein of novel goose parvovirus in baculovirus and evaluation of its immune effect. Microbial pathogenesis, 195, 106751. https://doi.org/10.1016/j.micpath.2024.106751
Toplam 75 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Viroloji
Bölüm Derleme
Yazarlar

Remziye Özbek 0000-0001-9831-7193

Erken Görünüm Tarihi 30 Haziran 2025
Yayımlanma Tarihi
Gönderilme Tarihi 4 Haziran 2025
Kabul Tarihi 24 Haziran 2025
Yayımlandığı Sayı Yıl 2025Cilt: 7 Sayı: 1

Kaynak Göster

APA Özbek, R. (2025). The Biotechnological Potential of Baculoviruses: From Insect Viruses to Biotechnology Workhorse. Turkish Veterinary Journal, 7(1), 19-30. https://doi.org/10.51755/turkvetj.1713887
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