Araştırma Makalesi
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Investigation of Paraoxonase and Ceruloplasmin Concentrations in Hair Goats Grazing at Different Heights

Yıl 2025, Cilt: 7 Sayı: 1, 6 - 10, 30.06.2025

Yeliz Kaya Kartal , Serdal Kurt , Funda Eşki , Seçkin Salar , İdil Baştan , Ayhan Baştan , Tevhide Sel

https://doi.org/10.51755/turkvetj.1691492

Öz

The aim of this study is to reveal the changes in Paraoxonase and Ceruloplasmin concentrations in hair goats grazed at different altitudes. 80 hair goats living in different regions in Adana were included in the study and divided into four groups. The first group was grazed at an altitude of 10-99 meters above sea level, the second group was grazed at an altitude of 240 meters, the third group was grazed at an altitude of 750-800 meters and the fourth group was grazed at an altitude of 1200-1500 meters. Blood samples collected from the jugular vein during the summer months were taken into red-capped tubes. Paraoxonase and Ceruloplasmin analyzes were analyzed by spectrophotometric method. The results of paraoxonase in 240 meter (1113.91±84.57 U/mL) and 750-800 meter (974.27±69.58 U/mL) were found statistically higher than 10-99 meter group (651.72±52.93 U/mL; p<0.01**). And the results of ceruloplasmin were found statistically different between 10-99 meter (10.15±0.63 U/mL) and 750-800 meter (7.13±0.46 U/mL), the 240 meter group (9.97±0.86 U/mL) and 750-800 meter group (7.13±0.46 U/mL) showed statistically significant difference too (p<0.05*). Studies have shown that antioxidant parameters of populations accustomed to living at high altitudes may be lower than those later moved to higher altitudes. In populations that normally live close to sea level and move to high altitudes later, their body defense systems are activated due to hypoxia, and antioxidant enzymes are expected to increase. It is thought that the reason why the highest ceruloplasmin concentration was observed in hair goats grazed at 10-99 meters may be that the temperature is higher at those levels and heat stress increases the acute phase proteins and likewise heat stress causes to a decrease in paraoxonase enzyme activity.

Anahtar Kelimeler

altitude antioxidant hair goats

Etik Beyan

This study was carried out with the permission of the Cukurova University, Local Ethics Committee of Ceyhan Faculty of Veterinary Medicine (Decision Date and Number: 12.12.2018 and 1/11).

Destekleyen Kurum

Cukurova University, Local Ethics Committee of Ceyhan Faculty of Veterinary Medicine

Kaynakça

  • Askew, E. W. (2002). Work at high altitude and oxidative stress: antioxidant nutrients. Toxic, 180, 107-119. DOI: 10.1016/s0300-483x(02)00385-2
  • Aviram, M. & Rosenblat, M. (2008). Paraoxonases (PON1, PON2, PON3) analyses in vitro and in vivo in relation to cardiovascular diseases. Methods Mol Biol, 477, 259-276.DOI: 10.1007/978-1-60327-517-020
  • Bakonyi, T. & Radak, Z. (2004). High altitude and free radicals. J Sport Sci Med, 3(2), 64-69.
  • Banerjee, D., Upadhyay, R. C., Chaudhary, U. B., Kumar, R., Singh, S., Ashutosh, G. J. M., Polley, S., Mukherjee, A., Das, T. K. & De, S. (2015). Seasonal variations in physio-biochemical profiles of Indian goats in the paradigm of hot and cold climate. Biol Rhythm Res, 46, 221-236. DOI: 10.1007/s12192-013-0469-0
  • Cabrera de Leon, A., Perez, M. C. R., Gonzalez, D. A., Díaz, B.B., Coello, S. D., Hernández, A. G. & Aguirre-Jaime,A.(2011). Hemodynamics and Metabolism at Lowversus Moderate Altitudes. High alt med biol, 12, 179-186.DOI: 10.1089/ham.2010.1078
  • Ceron, J. & Martinez-Subiela, S. (2004). An automated spectrophotometric method for measuring canine ceruloplasmin in serum. Vet Res, 35, 671–679.
  • Curzon, G. & Vallet, L. (1960). The Purification of Human Ceruloplasmin. Biochem J, 74, 279-287. DOI: 10.1051/vetres:2004046
  • Demirpence, O., Sevim, B., Yildirim, M., Ayan Nurlu, N., Mert, D. & Evliyaoğlu, O. (2014). Serum paraoxonase, TAS, TOS and ceruloplasmin in brucellosis. Int J Clin Exp Med, 7, 1592-1597.
  • Er, C. & Ok, M. (2020). Determination The Levels of Some Biochemical Parameters in Female Hair Goats in Cukurova Region. Kocatepe Vet J, 13, 25-29. DOI: 10.30607/kvj.623064
  • Field, A. (2013). Discovering Statistics Using Ibm Spss Statistics. 4th edn. Sage Publication Ltd.
  • Fleck, A. (1989). Clinical and nutritional aspects of changes in acute phase proteins during inflammation. Proc Nutr Soc, 48, 347–354. DOI: 10.1079/pns19890050
  • Gocmen, A. Y., Sahin, E., Semiz, E. & Gümuşlü, S. (2008). Is elevated serum ceruloplasmin level associated with increased risk of coronary artery disease? Can J Cardiol, 24, 209-212. DOI: 10.1016/s0828-282x(08)70586-5
  • Gungor, I., Alkoyak, K., Oz, S. & Koncagül, S. (2021). Growth, survival rate, and some reproductive characteristics of Hair goat under breeder conditions in Kahramanmaras Province. Turkish J of Vet and Anim Sci, 45, 1022-1029. DOI: 10.3906/vet-2104-51
  • Gunlu, A. & Alasahan, S. (2010). Evaluations on the Future of Goat Breeding in Turkey. Vet Hek Der Derg, 81, 15-20.
  • Halliwell, B., Murcia, M. A., Chirico, S. & Aruoma, O. I. (1995). Free radicals and antioxidants in food and in vivo: what they do and how they work. Crit Rev Food Sci Nutr, 35, 7-20. DOI: 10.1080/10408399509527682
  • Hirschler, V., Martin, M., Molinari, C., Botta, E., Tetzlaff, W. F. & Brites, F. (2019). Activity of Lipoprotein Associated Enzymes in Indigenous Children Living at Different Altitudes. Archives of Med Res, 50, 98-104. DOI: 10.1016/j.arcmed.2019.07.001
  • Hirschler, V., Martin, M., Oestreicher, K., Molinari, C., Tetzlaff, W., Botta, E., Boero, L., Brites, F. (2018). Activity of the antioxidant enzyme paraoxonase in Argentinean children living at high altitude. Redox Rep, 23, 35-40. DOI: 10.1080/13510002.2017.1370783
  • James, R. W. & Deakin, S. P. (2004). The importance of high-density lipoproteins for paraoxonase-1 secretion, stability, and activity. Free Radic Biol Med, 12, 1986–1994. DOI: 10.1016/j.freeradbiomed.2004.08.012
  • Kartal, Y. K., Kurt, S., Eski, F., Salar, S., Kismali, G., Baştan, A.& Sel, T. (2021). Alteration of paraoxonase,ceruloplasmin and immunoglobulin G levels in hairgoats at different ages. J Adv Vet Bio Sci Tech, 7, 7-12. DOI: 10.31797/vetbio.984872
  • Kaymakci, M., Taskin, T., Kosum, N., et al (2004). Organik süt üretimini Türkiye’de geliştirme olanakları. I. International Organic Animal Production and Food Safety Congress. Kusadası, 28 April-1 May 2004, 358-370.
  • Koyuncu, M. & Taskin, T. (2016). Organic Sheep and Goat Production. Hay Üret, 51, 56-62.
  • Kurt, S., Eski, F., Salar, S., Sel, T. & Baştan, A. (2021). The effect of heat stress on total oxidant capacity in hair goats. J of the Turkish Vet Med Soc, 92, 24-30. DOI: 10.33188/vetheder.771696 Mohammed, S. A., Razzaque, M. A., Omar, A. E., Albert, S. & Al-Gallaf, W. M. (2016). Biochemical and hematological profile of different breeds of goat maintained under intensive production system. African J of Biotech, 15, 1253-1257. DOI: 10.5897/AJB2016.15362
  • Muratsubaki, H., Enomoto, K., Ichijoh, Y. & Yamamoto, Y. (2003). Hypertriglyceridemia associated with decreased post-heparin plasma hepatic triglyceride lipase activity in hypoxic rats. Arch Physiol Biochem, 111, 449-454. DOI: 10.3109/13813450312331342319
  • Sinha, S., Ray, U. S., Saha, M., Singh, S. N. & Tomar, O. S. (2009c). Antioxidant and redox status after maximal aerobic exercise at high altitude in acclimatized lowlanders and native highlanders. Eur J Appl Physiol, 106, 807–814. DOI: 10.1007/s00421-009-1082-x
  • Sinha, S., Ray, U. S., Tomar, O. S. & Singh, S. N. (2009a). Different adaptation patterns of antioxidant system in natives and sojourners at high altitude. Respir Physiol Neurobiol, 167, 255-260. DOI: 10.1016/j.resp.2009.05.003
  • Sinha, S., Singh, S. N. & Ray, U. S. (2009b). Total Antioxidant Status at High Altitude in Lowlanders and Native Highlanders: Role of Uric Acid. High Alt Med Biol, 10, 269-274. DOI: 10.1089/ham.2008.1082
  • Yildirim, A., Sahin, Y. N., Turhan, H., Şen, İ. & Kaplan, İ. (2008). Dağcılarda yüksek rakıma maruz kalmanın bazı serum akut faz proteinleri üzerine etkisi. Fırat Tıp Derg, 13, 239-242.
  • Yilmaz, O., Kor, A., Ertugrul, M. & Wilson, R. T. (2012). The domestic livestock resources of Tukey: goat breed and types and their conservation status. Anim Gen Resourc, 51, 105-116. DOI: 10.1294/jes.23.47

"Farklı Yüksekliklerde Otlayan Kıl Keçilerinde Paraoksonaz ve Serüloplazmin Konsantrasyonlarının Araştırılması"

Yıl 2025, Cilt: 7 Sayı: 1, 6 - 10, 30.06.2025

Yeliz Kaya Kartal , Serdal Kurt , Funda Eşki , Seçkin Salar , İdil Baştan , Ayhan Baştan , Tevhide Sel

https://doi.org/10.51755/turkvetj.1691492

Öz

Bu çalışmanın amacı, farklı rakımlarda otlatılan kıl keçilerinde Paraoksonaz ve Serüloplazmin konsantrasyonlarındaki değişiklikleri ortaya koymaktır. Çalışmaya Adana'nın farklı bölgelerinde yaşayan 80 kıl keçisi dâhil edilmiş ve bu keçiler dört gruba ayrılmıştır. Birinci grup deniz seviyesinden 10-99 metre yükseklikte, ikinci grup 240 metre yükseklikte, üçüncü grup 750-800 metre yükseklikte ve dördüncü grup ise 1200-1500 metre yükseklikte otlatılmıştır. Yaz aylarında jugular venden alınan kan örnekleri kırmızı kapaklı tüplere alınmıştır. Paraoksonaz ve Serüloplazmin analizleri spektrofotometrik yöntemle gerçekleştirilmiştir. Paraoksonaz sonuçları, 240 metre (1113,91±84,57 U/mL) ve 750-800 metre (974,27±69,58 U/mL) gruplarında, 10-99 metre grubuna (651,72±52,93 U/mL; p<0,01**) göre istatistiksel olarak anlamlı şekilde yüksek bulunmuştur. Serüloplazmin sonuçlarında ise 10-99 metre (10,15±0,63 U/mL) ile 750-800 metre (7,13±0,46 U/mL) grubu arasında, ayrıca 240 metre (9,97±0,86 U/mL) ile 750-800 metre (7,13±0,46 U/mL) grubu arasında istatistiksel olarak anlamlı fark saptanmıştır (p<0,05*). Yapılan çalışmalar, yüksek rakımda yaşamaya alışık popülasyonlarda antioksidan parametrelerin, sonradan yüksek rakıma taşınanlara göre daha düşük olabileceğini göstermektedir. Normalde deniz seviyesine yakın yerlerde yaşayan ve sonradan yüksek rakımlara çıkan bireylerde, hipoksiye bağlı olarak vücut savunma sistemleri aktive olmakta ve buna bağlı olarak antioksidan enzim düzeylerinde artış beklenmektedir. 10-99 metre rakımda otlatılan kıl keçilerinde en yüksek serüloplazmin konsantrasyonunun gözlemlenmesinin, bu seviyelerde sıcaklığın daha yüksek olmasına ve buna bağlı olarak ısı stresinin akut faz proteinlerini artırmasına bağlanabileceği; benzer şekilde, ısı stresinin paraoksonaz enzim aktivitesinde azalmaya neden olduğu düşünülmektedir.

Anahtar Kelimeler

antioksidan kıl keçileri rakım

Kaynakça

  • Askew, E. W. (2002). Work at high altitude and oxidative stress: antioxidant nutrients. Toxic, 180, 107-119. DOI: 10.1016/s0300-483x(02)00385-2
  • Aviram, M. & Rosenblat, M. (2008). Paraoxonases (PON1, PON2, PON3) analyses in vitro and in vivo in relation to cardiovascular diseases. Methods Mol Biol, 477, 259-276.DOI: 10.1007/978-1-60327-517-020
  • Bakonyi, T. & Radak, Z. (2004). High altitude and free radicals. J Sport Sci Med, 3(2), 64-69.
  • Banerjee, D., Upadhyay, R. C., Chaudhary, U. B., Kumar, R., Singh, S., Ashutosh, G. J. M., Polley, S., Mukherjee, A., Das, T. K. & De, S. (2015). Seasonal variations in physio-biochemical profiles of Indian goats in the paradigm of hot and cold climate. Biol Rhythm Res, 46, 221-236. DOI: 10.1007/s12192-013-0469-0
  • Cabrera de Leon, A., Perez, M. C. R., Gonzalez, D. A., Díaz, B.B., Coello, S. D., Hernández, A. G. & Aguirre-Jaime,A.(2011). Hemodynamics and Metabolism at Lowversus Moderate Altitudes. High alt med biol, 12, 179-186.DOI: 10.1089/ham.2010.1078
  • Ceron, J. & Martinez-Subiela, S. (2004). An automated spectrophotometric method for measuring canine ceruloplasmin in serum. Vet Res, 35, 671–679.
  • Curzon, G. & Vallet, L. (1960). The Purification of Human Ceruloplasmin. Biochem J, 74, 279-287. DOI: 10.1051/vetres:2004046
  • Demirpence, O., Sevim, B., Yildirim, M., Ayan Nurlu, N., Mert, D. & Evliyaoğlu, O. (2014). Serum paraoxonase, TAS, TOS and ceruloplasmin in brucellosis. Int J Clin Exp Med, 7, 1592-1597.
  • Er, C. & Ok, M. (2020). Determination The Levels of Some Biochemical Parameters in Female Hair Goats in Cukurova Region. Kocatepe Vet J, 13, 25-29. DOI: 10.30607/kvj.623064
  • Field, A. (2013). Discovering Statistics Using Ibm Spss Statistics. 4th edn. Sage Publication Ltd.
  • Fleck, A. (1989). Clinical and nutritional aspects of changes in acute phase proteins during inflammation. Proc Nutr Soc, 48, 347–354. DOI: 10.1079/pns19890050
  • Gocmen, A. Y., Sahin, E., Semiz, E. & Gümuşlü, S. (2008). Is elevated serum ceruloplasmin level associated with increased risk of coronary artery disease? Can J Cardiol, 24, 209-212. DOI: 10.1016/s0828-282x(08)70586-5
  • Gungor, I., Alkoyak, K., Oz, S. & Koncagül, S. (2021). Growth, survival rate, and some reproductive characteristics of Hair goat under breeder conditions in Kahramanmaras Province. Turkish J of Vet and Anim Sci, 45, 1022-1029. DOI: 10.3906/vet-2104-51
  • Gunlu, A. & Alasahan, S. (2010). Evaluations on the Future of Goat Breeding in Turkey. Vet Hek Der Derg, 81, 15-20.
  • Halliwell, B., Murcia, M. A., Chirico, S. & Aruoma, O. I. (1995). Free radicals and antioxidants in food and in vivo: what they do and how they work. Crit Rev Food Sci Nutr, 35, 7-20. DOI: 10.1080/10408399509527682
  • Hirschler, V., Martin, M., Molinari, C., Botta, E., Tetzlaff, W. F. & Brites, F. (2019). Activity of Lipoprotein Associated Enzymes in Indigenous Children Living at Different Altitudes. Archives of Med Res, 50, 98-104. DOI: 10.1016/j.arcmed.2019.07.001
  • Hirschler, V., Martin, M., Oestreicher, K., Molinari, C., Tetzlaff, W., Botta, E., Boero, L., Brites, F. (2018). Activity of the antioxidant enzyme paraoxonase in Argentinean children living at high altitude. Redox Rep, 23, 35-40. DOI: 10.1080/13510002.2017.1370783
  • James, R. W. & Deakin, S. P. (2004). The importance of high-density lipoproteins for paraoxonase-1 secretion, stability, and activity. Free Radic Biol Med, 12, 1986–1994. DOI: 10.1016/j.freeradbiomed.2004.08.012
  • Kartal, Y. K., Kurt, S., Eski, F., Salar, S., Kismali, G., Baştan, A.& Sel, T. (2021). Alteration of paraoxonase,ceruloplasmin and immunoglobulin G levels in hairgoats at different ages. J Adv Vet Bio Sci Tech, 7, 7-12. DOI: 10.31797/vetbio.984872
  • Kaymakci, M., Taskin, T., Kosum, N., et al (2004). Organik süt üretimini Türkiye’de geliştirme olanakları. I. International Organic Animal Production and Food Safety Congress. Kusadası, 28 April-1 May 2004, 358-370.
  • Koyuncu, M. & Taskin, T. (2016). Organic Sheep and Goat Production. Hay Üret, 51, 56-62.
  • Kurt, S., Eski, F., Salar, S., Sel, T. & Baştan, A. (2021). The effect of heat stress on total oxidant capacity in hair goats. J of the Turkish Vet Med Soc, 92, 24-30. DOI: 10.33188/vetheder.771696 Mohammed, S. A., Razzaque, M. A., Omar, A. E., Albert, S. & Al-Gallaf, W. M. (2016). Biochemical and hematological profile of different breeds of goat maintained under intensive production system. African J of Biotech, 15, 1253-1257. DOI: 10.5897/AJB2016.15362
  • Muratsubaki, H., Enomoto, K., Ichijoh, Y. & Yamamoto, Y. (2003). Hypertriglyceridemia associated with decreased post-heparin plasma hepatic triglyceride lipase activity in hypoxic rats. Arch Physiol Biochem, 111, 449-454. DOI: 10.3109/13813450312331342319
  • Sinha, S., Ray, U. S., Saha, M., Singh, S. N. & Tomar, O. S. (2009c). Antioxidant and redox status after maximal aerobic exercise at high altitude in acclimatized lowlanders and native highlanders. Eur J Appl Physiol, 106, 807–814. DOI: 10.1007/s00421-009-1082-x
  • Sinha, S., Ray, U. S., Tomar, O. S. & Singh, S. N. (2009a). Different adaptation patterns of antioxidant system in natives and sojourners at high altitude. Respir Physiol Neurobiol, 167, 255-260. DOI: 10.1016/j.resp.2009.05.003
  • Sinha, S., Singh, S. N. & Ray, U. S. (2009b). Total Antioxidant Status at High Altitude in Lowlanders and Native Highlanders: Role of Uric Acid. High Alt Med Biol, 10, 269-274. DOI: 10.1089/ham.2008.1082
  • Yildirim, A., Sahin, Y. N., Turhan, H., Şen, İ. & Kaplan, İ. (2008). Dağcılarda yüksek rakıma maruz kalmanın bazı serum akut faz proteinleri üzerine etkisi. Fırat Tıp Derg, 13, 239-242.
  • Yilmaz, O., Kor, A., Ertugrul, M. & Wilson, R. T. (2012). The domestic livestock resources of Tukey: goat breed and types and their conservation status. Anim Gen Resourc, 51, 105-116. DOI: 10.1294/jes.23.47
Toplam 28 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Biyokimya
Bölüm Araştırma Makalesi
Yazarlar

Yeliz Kaya Kartal 0000-0002-3661-5504

Serdal Kurt 0000-0002-0191-3245

Funda Eşki 0000-0002-9242-9271

Seçkin Salar 0000-0001-9303-6253

İdil Baştan 0000-0003-2588-4036

Ayhan Baştan 0000-0001-8291-1147

Tevhide Sel 0000-0002-9753-779X

Erken Görünüm Tarihi 30 Haziran 2025
Yayımlanma Tarihi 30 Haziran 2025
Gönderilme Tarihi 4 Mayıs 2025
Kabul Tarihi 25 Haziran 2025
Yayımlandığı Sayı Yıl 2025Cilt: 7 Sayı: 1

Kaynak Göster

APA Kaya Kartal, Y., Kurt, S., Eşki, F., Salar, S., vd. (2025). Investigation of Paraoxonase and Ceruloplasmin Concentrations in Hair Goats Grazing at Different Heights. Turkish Veterinary Journal, 7(1), 6-10. https://doi.org/10.51755/turkvetj.1691492
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