Research Article
Year 2024,
, 12 - 16, 30.06.2024
Abstract
Bu çalışma ile daha önce insan virusları üzerinde antiviral etkinliği gösterilmiş olan son yılların popüler bitkisi stevianın içeriğindeki steviosidin BPIV-3 üzerindeki antiviral etkisi ilk kez araştırıldı. Ülke ekonomisi açısından önemli kayıplar oluşturan ve aşı ile istenilen kontrolün sağlanamadığı BPIV-3 enfeksiyonu ile mücadelede steviosidin bir seçenek olup olamayacağı elde edilen yeni verilerle ortaya kondu. Bu amaçla steviosidin MDBK hücre kültüründeki nonsitotoksik dozu sitotoksite testi ile belirlendikten sonra BPIV-3 üzerindeki antiviral etkisi stevia ile muamele edilen ve edilmeyen BPIV-3 titreleri, titrasyon testi ile belirlendi. Elde ettiğimiz sonuçlara göre, stevianın 10μmol/L ve nonsitotoksik doz olarak ölçülen 5000 µmol/L oranlarında uygulandığında, MDBK hücre kültüründe üretilen BPIV-3’ün titresini 10-3.7/0.1ml’ten sırasıyla 10-1/0.1 ml ve 100/0.1 ml’ye düşürerek antiviral etki gösterdiği tespit edildi.
Keywords
Ethical Statement
Bu çalışma “Hayvan Deneyleri Etik Kurullarının Çalışma Usul ve Esaslarına Dair Yönetmelik” Madde 8 (k) gereği HADYEK iznine tabi değildir.
Supporting Institution
Bu çalışma Ondokuz Mayıs Üniversitesi tarafından PYO.VET.1904.21.033 proje numarası ile desteklenmiştir.
Project Number
PYO.VET.1904.21.033
References
- Alfajaro M, Rho C, Kim J, Park Jun-G, Kim Deok-S, Hosmillo M, et al. (2014). Anti- rotavirus effects by combination therapy of stevioside and Sophora flavescens extract. Research in Veterinary Science, 96 (3), 567- 575.
- Behl T, Rocchetti G, Chadha S, Zengin G, Bungau S Kumar A et al.(2021). Phytochemicals from Plant Foods as Potential Source of Antiviral Agents: An Overview, Pharmaceuticals, 14 (4), 381.
- Ceole Ligia F, Companhoni P, Lopes S, Oliveira B, Gonçalves C, FilhoD, (2018). Anti-herpes activity of polysaccharide fractionsfrom Stevia rebaudiana leaves. Natural Product Research, 34 (11), 1558-1562.
- Frey HR, Liess B. (1971). Vermehrungskinetik und Verwendbarkeit eines stark zytopathogenen VD-MD Virusstammes für diagnostische Untersuchungen mit der Mikrotiter-Methode. Zentralblatt für Veterinärmedizin Reihe B, 18 (1), 61-71.
- Fulton RW, Neill, JD, Saliki JT, Landis C, Burge LJ, Payton ME, (2017). Genomic and antigenic characterization of bovine parainfluenza-3 viruses in the United States including modified live virus vaccine (MLV) strains and field strains from cattle. Virus Research, 235, 77–81.
- Gay E, Barnouin J, (2009). A nation-wide epidemiological study of acute bovine respiratory disease in France. Preventive Veterinary Medicine, 89, 265-271.
- Gümüşova S, Yazici Z, Albayrak H, Cakiroglu D (2007). Seroprevalence of bovine viral respiratory diseases. Acta Veterinaria, 57, 11-16.
- Gür S. (2019). Sığır, Koyun ve Keçilerin Bovine Parainfluenza 3 Virus Enfeksiyonuna Duyarlılıklarının Saha Şartlarında Serolojik Olarak Karşılaştırılması. Etlik Veteriner Mikrobiyoloji Dergisi, 30, 7- 13.
- Kedik SA., Yartsev EI, Stanishevskaya IE. (2009). Antiviral activity of dried extract of Stevia. Pharmaceutical Chemistry Journal, 43, 198 -199.
- Latarissa IR, Barliana, MI, Lestari K, (2020). A Comprehensive Review of Stevia rebaudiana Bertoni effects on Human Health and Its Mechanism. Journal of Advanced Pharmacy Education and Research, 10 (2), 91-95.
- Leal E, Liu C, Zhao Z, Deng Y, Villanov F, Liang L, Li J, Li S (2019). CuiIsolation of a Divergent Strain of Bovine Para influenzaVirus Type 3 (BPIV3) Infecting Cattle in China. Viruses, 11 (6), 489.
- Mathur S, Neha BPS, Singh, Shekhawat G (2017). Critical Review on Steviol Glycosides: Pharmacological, Toxicological and Therapeutic Aspects of High Potency Zero Caloric Sweetener International Journal of Pharmacology, 13, 7, 916- 928.
- Momtazi-Borojeni AA, Esmaeili SA, Abdollahi E, Sahebkar A (2017). A Review on the Pharmacology and Toxicology of Steviol Glycosides Extracted from Stevia rebaudiana. Current Pharmaceutical Design, 23 (11), 1616-1622.
- Oliveiraa B, Cordeirob MC, ̧Alvesa G, Coelec F, Tania U, Iacomini M (2013). Structure and antiviral activity of arabinogalactan with (1!6)-b-D-galactan core from Steviarebaudiana leaves. Carbohydrate Polymers, 94 (1), 179–184
- Ranjan R, Jaiswal J, Jena, J (2011). Stevia as a natural sweetener. International Journal of Pharmacology, 1 (4), 1199-1202.
- Sharma, N, Kumar P, Giri R (2021). Polysaccharides like pentagalloylglucose, parishin a and stevioside inhibits the viral entry by binding the Zika virus envelope protein. Journal of Biomolecular Structure and Dynamics, 39 (16): 6008-6020.
- Takahashi K, Matsuda M, Ohashi H, Taniguchi K, Nakagomi O, Abe Y, Mori S, Sato N, Okutani K, Shigeta S (2001). Analysis of anti-rotavirus activity of extract from Stevia rebaudiana. Antiviral Research, 49: 15 -24.
- Taleie N, Hamidoghli Y, Rabiei B, Hamidoghli S (2012). Effects of plant density and transplanting date on herbage, stevioside, phenol and flavonoid yield of Stevia rebaudiana Bertoni The International Journal of Agriculture and Crop Sciences, 4 (6): 298-302.
- Thrusfield M (2005). Veterinary epidemiology. 2nd Edition, Blackwell Science, Oxford, 117-198.
- Verma MK, Kumar N, Rishikant A (2019). Review on the pharmacological properties and medicinal use of Stevia rebaudiana. Journal of Pharmaceutical Innovation, 8 (1), 371- 374.
- Yazıcı Z, Gumusova S, Tamer C, Muftuoglu B, Ozan E, Arslan S, Bas O (2017). The first serological report for genotype C bovine parainfluenza 3 virus in ruminant species of mid-northern Turkey: traces from the past. Tropical Biomedicine, 36(3), 803–80
Year 2024,
, 12 - 16, 30.06.2024
Abstract
In this study, the antiviral effect of stevioside contained in stevia, a popular plant of recent years, which has previously been shown to be antiviral on human viruses, on BPIV-3 was investigated for the first time. With the new data obtained, it was revealed whether stevioside could be an option in the fight against BPIV-3 infection, which causes significant losses in terms of the national economy and cannot be controlled by vaccination. For this purpose, the nontitotoxic dose of stevioside in MDBK cell culture was determined by cytotoxicity test and the antiviral effect of stevioside on BPIV-3 was determined by titration test in BPIV-3 titers treated and untreated with stevia. According to our results, it was determined that stevia showed an antiviral effect by decreasing the titer of BPIV-3 produced in MDBK cell culture from 10-3.7/0.1ml to 10-1/0.1ml and 100/0.1ml, respectively, when applied at 10μmol/L and 5000 µmol/L, which was measured as noncytotoxic dose.
Keywords
Project Number
PYO.VET.1904.21.033
References
- Alfajaro M, Rho C, Kim J, Park Jun-G, Kim Deok-S, Hosmillo M, et al. (2014). Anti- rotavirus effects by combination therapy of stevioside and Sophora flavescens extract. Research in Veterinary Science, 96 (3), 567- 575.
- Behl T, Rocchetti G, Chadha S, Zengin G, Bungau S Kumar A et al.(2021). Phytochemicals from Plant Foods as Potential Source of Antiviral Agents: An Overview, Pharmaceuticals, 14 (4), 381.
- Ceole Ligia F, Companhoni P, Lopes S, Oliveira B, Gonçalves C, FilhoD, (2018). Anti-herpes activity of polysaccharide fractionsfrom Stevia rebaudiana leaves. Natural Product Research, 34 (11), 1558-1562.
- Frey HR, Liess B. (1971). Vermehrungskinetik und Verwendbarkeit eines stark zytopathogenen VD-MD Virusstammes für diagnostische Untersuchungen mit der Mikrotiter-Methode. Zentralblatt für Veterinärmedizin Reihe B, 18 (1), 61-71.
- Fulton RW, Neill, JD, Saliki JT, Landis C, Burge LJ, Payton ME, (2017). Genomic and antigenic characterization of bovine parainfluenza-3 viruses in the United States including modified live virus vaccine (MLV) strains and field strains from cattle. Virus Research, 235, 77–81.
- Gay E, Barnouin J, (2009). A nation-wide epidemiological study of acute bovine respiratory disease in France. Preventive Veterinary Medicine, 89, 265-271.
- Gümüşova S, Yazici Z, Albayrak H, Cakiroglu D (2007). Seroprevalence of bovine viral respiratory diseases. Acta Veterinaria, 57, 11-16.
- Gür S. (2019). Sığır, Koyun ve Keçilerin Bovine Parainfluenza 3 Virus Enfeksiyonuna Duyarlılıklarının Saha Şartlarında Serolojik Olarak Karşılaştırılması. Etlik Veteriner Mikrobiyoloji Dergisi, 30, 7- 13.
- Kedik SA., Yartsev EI, Stanishevskaya IE. (2009). Antiviral activity of dried extract of Stevia. Pharmaceutical Chemistry Journal, 43, 198 -199.
- Latarissa IR, Barliana, MI, Lestari K, (2020). A Comprehensive Review of Stevia rebaudiana Bertoni effects on Human Health and Its Mechanism. Journal of Advanced Pharmacy Education and Research, 10 (2), 91-95.
- Leal E, Liu C, Zhao Z, Deng Y, Villanov F, Liang L, Li J, Li S (2019). CuiIsolation of a Divergent Strain of Bovine Para influenzaVirus Type 3 (BPIV3) Infecting Cattle in China. Viruses, 11 (6), 489.
- Mathur S, Neha BPS, Singh, Shekhawat G (2017). Critical Review on Steviol Glycosides: Pharmacological, Toxicological and Therapeutic Aspects of High Potency Zero Caloric Sweetener International Journal of Pharmacology, 13, 7, 916- 928.
- Momtazi-Borojeni AA, Esmaeili SA, Abdollahi E, Sahebkar A (2017). A Review on the Pharmacology and Toxicology of Steviol Glycosides Extracted from Stevia rebaudiana. Current Pharmaceutical Design, 23 (11), 1616-1622.
- Oliveiraa B, Cordeirob MC, ̧Alvesa G, Coelec F, Tania U, Iacomini M (2013). Structure and antiviral activity of arabinogalactan with (1!6)-b-D-galactan core from Steviarebaudiana leaves. Carbohydrate Polymers, 94 (1), 179–184
- Ranjan R, Jaiswal J, Jena, J (2011). Stevia as a natural sweetener. International Journal of Pharmacology, 1 (4), 1199-1202.
- Sharma, N, Kumar P, Giri R (2021). Polysaccharides like pentagalloylglucose, parishin a and stevioside inhibits the viral entry by binding the Zika virus envelope protein. Journal of Biomolecular Structure and Dynamics, 39 (16): 6008-6020.
- Takahashi K, Matsuda M, Ohashi H, Taniguchi K, Nakagomi O, Abe Y, Mori S, Sato N, Okutani K, Shigeta S (2001). Analysis of anti-rotavirus activity of extract from Stevia rebaudiana. Antiviral Research, 49: 15 -24.
- Taleie N, Hamidoghli Y, Rabiei B, Hamidoghli S (2012). Effects of plant density and transplanting date on herbage, stevioside, phenol and flavonoid yield of Stevia rebaudiana Bertoni The International Journal of Agriculture and Crop Sciences, 4 (6): 298-302.
- Thrusfield M (2005). Veterinary epidemiology. 2nd Edition, Blackwell Science, Oxford, 117-198.
- Verma MK, Kumar N, Rishikant A (2019). Review on the pharmacological properties and medicinal use of Stevia rebaudiana. Journal of Pharmaceutical Innovation, 8 (1), 371- 374.
- Yazıcı Z, Gumusova S, Tamer C, Muftuoglu B, Ozan E, Arslan S, Bas O (2017). The first serological report for genotype C bovine parainfluenza 3 virus in ruminant species of mid-northern Turkey: traces from the past. Tropical Biomedicine, 36(3), 803–80
There are 21 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Veterinary Virology |
| Journal Section | Research Article |
| Authors | |
| Project Number | PYO.VET.1904.21.033 |
| Early Pub Date | June 30, 2024 |
| Publication Date | June 30, 2024 |
| Submission Date | April 29, 2024 |
| Acceptance Date | June 10, 2024 |
| Published in Issue | Year 2024 |