Bulaşıcı Veneral Tümör (BVT) oldukça bulaşıcı, yuvarlak hücreli bir tümördür ve tümörün kökeni bilinmemektedir. Bu çalışmamızda BVT’nin hücresel karakterizasyonu ortaya koymak amacıyla CD3, CD79αcy, vimentin, α-düz kas aktin, s100 and sitokeratin 5/6 ekspresyonlarını immunohistokimyasal olarak değerlendirmeyi amaçladık. Bu çalışmanın materyalini altısı dişi ve dördü erkek olmak üzere toplamda on adet BVT teşhisi konulmuş kesit oluşturdu. Köpeklerden alınan doku kesitleri %10’luk tamponlu formaldehit solüsyonunda tespit edildi. Rutin doku işlemleri ardından kesitlere Hematoksilen & Eozin boyası uygulandı. Kesitler ışık mikroskobu altında değerlendirildi. Sitolojik incelemeler için kitlelerden alınan smearlere Diff-quick metodu uygulandı. İmmunohistokimyasal boyama olarak avidin biotin immune peroksidaz kompleks yöntemi uygulandı. Makrosobik, histopatolojik ve sitolojik bulgular temelinde vakalara TVT teşhisi konuldu. Tüm vakalar yuvarlak tümör hücrelerinde vimentin ekspresyonu dışında tüm belirteçler için negatifti. CD3, CD79αcy, s100, sitokeratin 5/6 and α-düz kas aktin immunoreaktivitesi açısından negatiflik tümörün kökeninin lenfositer, düz kas hücresi ya da epitelyal kökenli olmadığını düşündürmektedir. Geriye kalan tek seçenek tümörün histiyositer kökenli olabileceği yönündedir.
Transmissible Venereal Tumor (TVT) is highly contagious round cell tumor and its origin is unknown. In our study, we aimed to evaluate CD3, CD79αcy, vimentin, α-smooth muscle actin, s100 and cytokeratin 5/6 expressions immunohistochemically in order to reveal the cellular characterization of TVT. The material of this study consisted of ten dog tissue sections, six females and four males diagnosed with TVT. Tissue samples from dog were fixed in a 10% buffered formaldehyde solution. After routine tissue procedures follow-up, Hematoxylin & Eosin stain was applied to the sections and investigated under a light microscope. The smear prepared from tumor was examined with the Diff-quick method for cytologically. Immunohistochemical staining was performed on the tissues using the avidin-biotin immune peroxidase complex method. Based on macroscopic, histopathological and cytological findings, we decided that the cases were TVT. All cases were negative for all markers except vimentin expression in round tumoral cells. The negative immunoreactivity of CD3, CD79αcy, α-smooth muscle actin and cytokeratin 5/6 in tumor cells suggests that the origin of the tumor is not lymphocytic, smooth muscle cells or epithelial. Only the option of histiocytic origin remains.
Primary Language | English |
---|---|
Subjects | Veterinary |
Journal Section | Research Article |
Authors |
|
Publication Date | December 30, 2020 |
Published in Issue | Year 2020Volume: 2 Issue: 2 |
Bibtex | @research article { turkvetj763321, journal = {Turkish Veterinary Journal}, eissn = {2667-8292}, address = {Sivas Cumhuriyet Üniversitesi, Veteriner Fakültesi, SİVAS}, publisher = {Sivas Cumhuriyet Üniversitesi}, year = {2020}, volume = {2}, number = {2}, pages = {54 - 61}, title = {CD3, CD79αcy, Vimentin, α-Smooth Muscle Actin, S100 and Cytokeratin 5/6 Expression in Immunohistochemical Characterization of Dogs with Transmissible Venereal Tumors}, key = {cite}, author = {Karakurt, Emin and Kuru, Mushap and Beytut, Enver and Dağ, Serpil and Nuhoğlu, Hilmi and Demir, Murat Can and Yıldız, Ayfer and Kaçar, Cihan} } |
APA | Karakurt, E. , Kuru, M. , Beytut, E. , Dağ, S. , Nuhoğlu, H. , Demir, M. C. , Yıldız, A. & Kaçar, C. (2020). CD3, CD79αcy, Vimentin, α-Smooth Muscle Actin, S100 and Cytokeratin 5/6 Expression in Immunohistochemical Characterization of Dogs with Transmissible Venereal Tumors . Turkish Veterinary Journal , 2 (2) , 54-61 . Retrieved from http://tvj.cumhuriyet.edu.tr/en/pub/issue/58876/763321 |
MLA | Karakurt, E. , Kuru, M. , Beytut, E. , Dağ, S. , Nuhoğlu, H. , Demir, M. C. , Yıldız, A. , Kaçar, C. "CD3, CD79αcy, Vimentin, α-Smooth Muscle Actin, S100 and Cytokeratin 5/6 Expression in Immunohistochemical Characterization of Dogs with Transmissible Venereal Tumors" . Turkish Veterinary Journal 2 (2020 ): 54-61 <http://tvj.cumhuriyet.edu.tr/en/pub/issue/58876/763321> |
Chicago | Karakurt, E. , Kuru, M. , Beytut, E. , Dağ, S. , Nuhoğlu, H. , Demir, M. C. , Yıldız, A. , Kaçar, C. "CD3, CD79αcy, Vimentin, α-Smooth Muscle Actin, S100 and Cytokeratin 5/6 Expression in Immunohistochemical Characterization of Dogs with Transmissible Venereal Tumors". Turkish Veterinary Journal 2 (2020 ): 54-61 |
RIS | TY - JOUR T1 - CD3, CD79αcy, Vimentin, α-Smooth Muscle Actin, S100 and Cytokeratin 5/6 Expression in Immunohistochemical Characterization of Dogs with Transmissible Venereal Tumors AU - EminKarakurt, MushapKuru, EnverBeytut, SerpilDağ, HilmiNuhoğlu, Murat CanDemir, AyferYıldız, CihanKaçar Y1 - 2020 PY - 2020 N1 - DO - T2 - Turkish Veterinary Journal JF - Journal JO - JOR SP - 54 EP - 61 VL - 2 IS - 2 SN - -2667-8292 M3 - UR - Y2 - 2020 ER - |
EndNote | %0 Turkish Veterinary Journal CD3, CD79αcy, Vimentin, α-Smooth Muscle Actin, S100 and Cytokeratin 5/6 Expression in Immunohistochemical Characterization of Dogs with Transmissible Venereal Tumors %A Emin Karakurt , Mushap Kuru , Enver Beytut , Serpil Dağ , Hilmi Nuhoğlu , Murat Can Demir , Ayfer Yıldız , Cihan Kaçar %T CD3, CD79αcy, Vimentin, α-Smooth Muscle Actin, S100 and Cytokeratin 5/6 Expression in Immunohistochemical Characterization of Dogs with Transmissible Venereal Tumors %D 2020 %J Turkish Veterinary Journal %P -2667-8292 %V 2 %N 2 %R %U |
ISNAD | Karakurt, Emin , Kuru, Mushap , Beytut, Enver , Dağ, Serpil , Nuhoğlu, Hilmi , Demir, Murat Can , Yıldız, Ayfer , Kaçar, Cihan . "CD3, CD79αcy, Vimentin, α-Smooth Muscle Actin, S100 and Cytokeratin 5/6 Expression in Immunohistochemical Characterization of Dogs with Transmissible Venereal Tumors". Turkish Veterinary Journal 2 / 2 (December 2020): 54-61 . |
AMA | Karakurt E. , Kuru M. , Beytut E. , Dağ S. , Nuhoğlu H. , Demir M. C. , Yıldız A. , Kaçar C. CD3, CD79αcy, Vimentin, α-Smooth Muscle Actin, S100 and Cytokeratin 5/6 Expression in Immunohistochemical Characterization of Dogs with Transmissible Venereal Tumors. Turkish Veterinary Journal. 2020; 2(2): 54-61. |
Vancouver | Karakurt E. , Kuru M. , Beytut E. , Dağ S. , Nuhoğlu H. , Demir M. C. , Yıldız A. , Kaçar C. CD3, CD79αcy, Vimentin, α-Smooth Muscle Actin, S100 and Cytokeratin 5/6 Expression in Immunohistochemical Characterization of Dogs with Transmissible Venereal Tumors. Turkish Veterinary Journal. 2020; 2(2): 54-61. |
IEEE | E. Karakurt , M. Kuru , E. Beytut , S. Dağ , H. Nuhoğlu , M. C. Demir , A. Yıldız and C. Kaçar , "CD3, CD79αcy, Vimentin, α-Smooth Muscle Actin, S100 and Cytokeratin 5/6 Expression in Immunohistochemical Characterization of Dogs with Transmissible Venereal Tumors", Turkish Veterinary Journal, vol. 2, no. 2, pp. 54-61, Dec. 2020 |