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Modifiye U-synch Protokolü ile Mevsim Dışı Senkronize Edilen Akkaraman Kangal Irkı Prepubertal Kuzu ve Primipar Koyunlara GnRH ve hCG Uygulamalarının Fertilite Üzerine Etkisi

Year 2019, Volume: 1 Issue: 2, 60 - 68, 15.08.2019

Abdurrahman Takcı

Abstract

Koyunlar mevsimsel poliöstrik hayvanlardır. Yerli gen kaynağımız olan Akkaraman-Kangal ırkı koyunlar da yıl içinde sadece üreme sezonunda (sonbahar aylarında) seksüel aktivite göstermektedir. Bundan dolayı üreme sezonu dışında (anöstrüste) gebelik elde etmek için farklı seksüel uyarımlar yapılması gerekmektedir. Bu amaçla mevcut çalışma her grubun 15 baş prepubertal kuzu (10-14 aylık) ve 10 baş primipar koyun (postpartum 55-70.günlerinde olan) ihtiva ettiği üç grup ile derin anöstrüs döneminde (Mayıs-Haziran) olan hayvanlarda yürütülmüştür. Bütün gruplara 0. gün 100 μg gonadorelin diasetat tetrahidrat kas içi enjeksiyonu ile birlikte 20 mg flurogestan asetat içeren sponj 7 gün süreyle vaginada tutulmuş ve sponj çıkarıldığı gün 131,5 μg kloprostenol sodyum ve 600 IU eCG kas içi enjeksiyonları yapılmıştır. Sponj çıkarıldıktan 56 saat sonra (10. gün); Grup 1’deki hayvanlara 100 μg gonadorelin diasetat tetrahidrat (GnRH), Grup 2’deki 600 IU hCG enjeksiyonu yapılırken, Grup 3 (Kontrol)’deki hayvanlara 2 ml serum fizyolojik enjeksiyonlarını takiben koç katımı yapıldı ve koçlar 5 gün boyunca (10-15. gün)  koyunlarla birlikte kaldı. Gruplar arasında östrus gösterme, gebe kalma oranları ve eşit günlerde progesteron seviyeleri değerlendirilmiş olup gruplar arasında bu parametreler yönünden oluşan fark istatistiki yönden anlamlı bulunmadı. Modifiye U-synch yöntemlerinin prepubertal toklu ve primipar koyunlarda reproduktif etkinliği benzer bulundu. Sonuç olarak Akkaraman Kangal ırkı prepubertal toklu ve primipar koyunlarda modifiye U-synch yöntemleri ile anöstrüste gebelik elde edilebileceği belirlenmiştir. 

Keywords

Akkaraman anöstrus kangal prepubertal prepubertal koyun

References

  • Abecia JA, Chemineau P, Gómez A, Keller M, Forcada F, Delgadillo JA (2016) Presence of photoperiod-melatonin-induced, sexually-activated rams in spring advances puberty in autumn-born ewe lambs. Anim Reprod Sci 170: 114-120. https://doi.org/ 10.1016/j.anireprosci.2016.04.011.
  • Abecia JA, Forcada F, González-Bulnes A (2012) Hormonal control of reproduction in small ruminants. Anım Reprod Scı 130(3-4): 173-179. https://doi: 10.1016/j.anireprosci.2012.01.011
  • Bartlewski PM, Baby TE, Giffin JL (2011) Reproductive cycles in sheep. Anım Reprod Scı 124(3-4): 259-268. https:// doi: 10.1016/j.anireprosci.2011.02.024
  • Bartlewski PM, Beard AP, Cook SJ, Rawlings NC (1998) Ovarian follicular dynamics during anoestrus in ewes. J Reprod Fertil 113(2): 275-285. https://doi.org/10.1530/jrf.0.1130275
  • Dias L MK, Sales JNS, Viau P, Barros MBP, Nicolau SS, Simões LMS, Oliveira CA (2018) Although it induces synchronized ovulation, hCG reduces the fertility of Santa Ines ewes submitted to TAI. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 70(1): 122-130. http://dx.doi.org/10.1590/1678-4162-9679
  • Edwards SJ, Juengel JL, O’Connell AR, Johnstone PD, Farquhar PA, Davis, GH (2015) Attainment of puberty by ewes in the first year of life is associated with improved reproductive performance at 2 years of age. Small Rumin Res 123(1): 118-123. https://doi.org/10.1016/j.smallrumres.2014.11.006
  • Eppleston J, Evans G, Roberts EM (1991) Effect of time of PMSG and GnRH on the time of ovulation, LH secretion and reproductive performance after intrauterine insemination with frozen ram semen. Anim Reprod Sci 26: 227–237. https://doi.org/10.1016/0378-4320(91)90049-6
  • Foster DL, Hileman SM (2015) Puberty in the sheep. In: Plant TM, Zeleznik AJ (ed) knobil and neill's physiology of reproduction (Vol. 2), 3rd edn. Academic Press Elseiver, London, pp 1441-1485.
  • Ginther OJ, Beg MA, Gastal EL, Gastal MO, Cooper DA (2009) Treatment with human chorionic gonadotropin (hCG) for ovulation induction is associated with an immediate 17β-estradiol decrease and a more rapid LH increase in mares. Anim Reprod Sci 114(1-3): 311-317. https:// doi: 10.1016/j.anireprosci.2008.08.026
  • Gómez-Brunet A, Santiago-Moreno J, Campo AD, Malpaux B, Chemineau P, Tortonese DJ, LópezSebastián A (2008) Endogenous circannual cycles of ovarian activity and changes in prolactin and melatonin secretion in wild and domestic female sheep maintained under a long-day photoperiod. Biol Reprod 78(3): 552-562. https:// doi 10.1095/biolreprod.107.064394
  • İbiş M, Ağaoğlu AR (2016) Koyun ve keçilerde üremenin senkronizasyonu. MAE Vet Fak Derg 1(2): 47-53.
  • Kassem R, Owen JB, Fadel I (1989) The effect of pre-mating nutrition and exposure to the presence of rams on the onset of puberty in Awassi ewe lambs under semi-arid conditions. Animal Sci 48(2): 393-397. https://doi.org/10.1017/S0003356100040381
  • Kıvrak (2019). Prepubertal dişi kuzularda aşım sezonu dışında uygulanan sülpirid’in reprodüktif etkisinin belirlenmesi. Doktora tezi. Selçuk Üniversitesi Sağlık bilimleri enstitüsü
  • Knight TW, Tervit HR, Lynch PR (1983) Effects of boar pheromones, ram's wool and presence of bucks on ovarian activity in anovular ewes early in the breeding season. Anım Reprod Scı 6(2): 129-134. https://doi.org/10.1016/0378-4320(83)90017-9
  • Lapthorn AJ, Harris DC, Littlejohn A, Lustbader JW, Canfield RE, Machin KJ, Isaacs NW (1994) Crystal structure of human chorionic gonadotropin. Nature 369(6480): 455.
  • Lewis RM, Notter DR, Hogue DE, Magee BH (1996) Ewe fertility in the STAR accelerated lambing system. J Anim Sci 74(7): 1511-1522. https://doi.org/10.2527/1996.7471511x
  • Özyurtlu N, Bademkıran S (2010) Koyunlarda östrus senkronizasyonu ve östrusu uyarma yöntemleri. Dicle Üniv Vet Fak Derg (1): 17-22.
  • Pampori ZA, Sheikh AA, Aarif O, Hasin D, Bhat IA (2018) Physiology of reproductive seasonality in sheep–an update. Biol Rhythm Res 1-13. https://doi.org/10.1080/09291016.2018.1548112
  • Ungerfeld R (2011) Combination of the ram effect with PGF2α estrous synchronization treatment in ewes during the breeding season. Anim Reprod Sci 124: 65-68. https://doi.org/10,1016/j.anireprosci.2011.02.021
  • Reyna J, Thomson PC, Evans G, Maxwell WM (2007) Synchrony of ovulation and follicular dynamics in merino ewes treated with GnRH in the breeding and non-breeding seasons. Reprod. Domest. Anim. 42: 410-417 https://doi.org/ 10.1111/j.1439-0531.2006.00800.x
  • Simões J (2015) Recent advances on synchronization of ovulation in goats, out of season, for a more sustainable production. Asia Pac J Rural Dev 4(2): 157-165. https://doi.org/10.1016/S2305-0500(15)30014-2
  • Valasi I, Chadio S, Fthenakis GC, Amiridis GS (2012) Management of pre-pubertal small ruminants: Physiological basis and clinical approach. Anim Reprod Sci 130(3-4): 126-134. . https:// doi: 10.1016/j.anireprosci.2012.01.005
  • Walker SK, Smith DH, Godfrey B, Seamark RF (1989) Time of ovulation in the South Australian Merino ewe following synchronization of estrus. 1. Variation within and between flocks. Theriogenology 31: 545–553. https://doi.org/10.1016/0093-691X(89)90239-2
  • Weems PW, Goodman RL, Lehman MN (2015) Neural mechanisms controlling seasonal reproduction: principles derived from the sheep model and its comparison with hamsters. Front Neuroendocrinol 37: 43-51. https:// doi: 10.1016/j.yfrne.2014.12.002
  • Wildeus S (2000) Current concepts in synchronization of estrus: Sheep and goats 1, 2. J Anim Sci 77(E-Suppl): 1-14. https:// doi: 10.2527/jas2000.00218812007700ES0040x
  • Zamiri MJ, Hosseini M (1998) Effects of human chorionic gonadotropin (hCG) and phenobarbital on the reproductive performance of fat-tailed Ghezel ewes. Small Rumin Res 30(3): 157-161. https://doi.org/10.1016/S0921-4488(98)00099-6

The Effects of GnRH and hCG Applications on Fertility in Akkaraman Kangal Prepubertal Lambs and Primiparous Sheep with Synchronized Modified U-synch Protocol and out of Breeding Season

Year 2019, Volume: 1 Issue: 2, 60 - 68, 15.08.2019

Abdurrahman Takcı

Abstract

Sheep are seasonally polyestrous. Our native genetic source Akkaraman-Kangal breed sheep show sexual activity only in breeding season (months of fall) throught year too. Diffirent induction methods are needed to produce lamb out of breeding season). For this purpose, the study conducted on 3 groups which involve 15 prepubertal ewe lamb (10-14 months of age) and 10 primiparous ewe (55-70 days of postpartum) in deep anestrous (may-june). Each group was recieved 100 µg gonadorelin diacetate tetrahydrate (GnRH) together with progestrone including sponge (20 mg flugeston acetate). Sponge was out 7 days (7th Day) later following 131,5 µg PGF2α and 600 IU eCG injection.  100 µg gonadorelin diacetate tetrahydrate (GnRH), 600 IU hCG and 2 ml sterile saline solution was injected to group 1, 2 and 3 respectively at the 56th hour following sponge out. Ram were introduced to the groups for 5 days after injection (between 10-15th days). Progestrone levels, Estrous and pregnancy rate were evaluted. Significant diffirences was not observed between groups. Reproductive efficiency of the modified U-synch method found to be smilar in prepubertal ewe lambs and primiparous ewes.  In conclusion, it was determined that a reasonable pregnancy rate can be achieved with modified U-synch method in Akkaraman-Kangal prepubertal ewe lambs and primiparous ewes.

Keywords

Akkaraman anestrus kangal prepubertal sheep

References

  • Abecia JA, Chemineau P, Gómez A, Keller M, Forcada F, Delgadillo JA (2016) Presence of photoperiod-melatonin-induced, sexually-activated rams in spring advances puberty in autumn-born ewe lambs. Anim Reprod Sci 170: 114-120. https://doi.org/ 10.1016/j.anireprosci.2016.04.011.
  • Abecia JA, Forcada F, González-Bulnes A (2012) Hormonal control of reproduction in small ruminants. Anım Reprod Scı 130(3-4): 173-179. https://doi: 10.1016/j.anireprosci.2012.01.011
  • Bartlewski PM, Baby TE, Giffin JL (2011) Reproductive cycles in sheep. Anım Reprod Scı 124(3-4): 259-268. https:// doi: 10.1016/j.anireprosci.2011.02.024
  • Bartlewski PM, Beard AP, Cook SJ, Rawlings NC (1998) Ovarian follicular dynamics during anoestrus in ewes. J Reprod Fertil 113(2): 275-285. https://doi.org/10.1530/jrf.0.1130275
  • Dias L MK, Sales JNS, Viau P, Barros MBP, Nicolau SS, Simões LMS, Oliveira CA (2018) Although it induces synchronized ovulation, hCG reduces the fertility of Santa Ines ewes submitted to TAI. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 70(1): 122-130. http://dx.doi.org/10.1590/1678-4162-9679
  • Edwards SJ, Juengel JL, O’Connell AR, Johnstone PD, Farquhar PA, Davis, GH (2015) Attainment of puberty by ewes in the first year of life is associated with improved reproductive performance at 2 years of age. Small Rumin Res 123(1): 118-123. https://doi.org/10.1016/j.smallrumres.2014.11.006
  • Eppleston J, Evans G, Roberts EM (1991) Effect of time of PMSG and GnRH on the time of ovulation, LH secretion and reproductive performance after intrauterine insemination with frozen ram semen. Anim Reprod Sci 26: 227–237. https://doi.org/10.1016/0378-4320(91)90049-6
  • Foster DL, Hileman SM (2015) Puberty in the sheep. In: Plant TM, Zeleznik AJ (ed) knobil and neill's physiology of reproduction (Vol. 2), 3rd edn. Academic Press Elseiver, London, pp 1441-1485.
  • Ginther OJ, Beg MA, Gastal EL, Gastal MO, Cooper DA (2009) Treatment with human chorionic gonadotropin (hCG) for ovulation induction is associated with an immediate 17β-estradiol decrease and a more rapid LH increase in mares. Anim Reprod Sci 114(1-3): 311-317. https:// doi: 10.1016/j.anireprosci.2008.08.026
  • Gómez-Brunet A, Santiago-Moreno J, Campo AD, Malpaux B, Chemineau P, Tortonese DJ, LópezSebastián A (2008) Endogenous circannual cycles of ovarian activity and changes in prolactin and melatonin secretion in wild and domestic female sheep maintained under a long-day photoperiod. Biol Reprod 78(3): 552-562. https:// doi 10.1095/biolreprod.107.064394
  • İbiş M, Ağaoğlu AR (2016) Koyun ve keçilerde üremenin senkronizasyonu. MAE Vet Fak Derg 1(2): 47-53.
  • Kassem R, Owen JB, Fadel I (1989) The effect of pre-mating nutrition and exposure to the presence of rams on the onset of puberty in Awassi ewe lambs under semi-arid conditions. Animal Sci 48(2): 393-397. https://doi.org/10.1017/S0003356100040381
  • Kıvrak (2019). Prepubertal dişi kuzularda aşım sezonu dışında uygulanan sülpirid’in reprodüktif etkisinin belirlenmesi. Doktora tezi. Selçuk Üniversitesi Sağlık bilimleri enstitüsü
  • Knight TW, Tervit HR, Lynch PR (1983) Effects of boar pheromones, ram's wool and presence of bucks on ovarian activity in anovular ewes early in the breeding season. Anım Reprod Scı 6(2): 129-134. https://doi.org/10.1016/0378-4320(83)90017-9
  • Lapthorn AJ, Harris DC, Littlejohn A, Lustbader JW, Canfield RE, Machin KJ, Isaacs NW (1994) Crystal structure of human chorionic gonadotropin. Nature 369(6480): 455.
  • Lewis RM, Notter DR, Hogue DE, Magee BH (1996) Ewe fertility in the STAR accelerated lambing system. J Anim Sci 74(7): 1511-1522. https://doi.org/10.2527/1996.7471511x
  • Özyurtlu N, Bademkıran S (2010) Koyunlarda östrus senkronizasyonu ve östrusu uyarma yöntemleri. Dicle Üniv Vet Fak Derg (1): 17-22.
  • Pampori ZA, Sheikh AA, Aarif O, Hasin D, Bhat IA (2018) Physiology of reproductive seasonality in sheep–an update. Biol Rhythm Res 1-13. https://doi.org/10.1080/09291016.2018.1548112
  • Ungerfeld R (2011) Combination of the ram effect with PGF2α estrous synchronization treatment in ewes during the breeding season. Anim Reprod Sci 124: 65-68. https://doi.org/10,1016/j.anireprosci.2011.02.021
  • Reyna J, Thomson PC, Evans G, Maxwell WM (2007) Synchrony of ovulation and follicular dynamics in merino ewes treated with GnRH in the breeding and non-breeding seasons. Reprod. Domest. Anim. 42: 410-417 https://doi.org/ 10.1111/j.1439-0531.2006.00800.x
  • Simões J (2015) Recent advances on synchronization of ovulation in goats, out of season, for a more sustainable production. Asia Pac J Rural Dev 4(2): 157-165. https://doi.org/10.1016/S2305-0500(15)30014-2
  • Valasi I, Chadio S, Fthenakis GC, Amiridis GS (2012) Management of pre-pubertal small ruminants: Physiological basis and clinical approach. Anim Reprod Sci 130(3-4): 126-134. . https:// doi: 10.1016/j.anireprosci.2012.01.005
  • Walker SK, Smith DH, Godfrey B, Seamark RF (1989) Time of ovulation in the South Australian Merino ewe following synchronization of estrus. 1. Variation within and between flocks. Theriogenology 31: 545–553. https://doi.org/10.1016/0093-691X(89)90239-2
  • Weems PW, Goodman RL, Lehman MN (2015) Neural mechanisms controlling seasonal reproduction: principles derived from the sheep model and its comparison with hamsters. Front Neuroendocrinol 37: 43-51. https:// doi: 10.1016/j.yfrne.2014.12.002
  • Wildeus S (2000) Current concepts in synchronization of estrus: Sheep and goats 1, 2. J Anim Sci 77(E-Suppl): 1-14. https:// doi: 10.2527/jas2000.00218812007700ES0040x
  • Zamiri MJ, Hosseini M (1998) Effects of human chorionic gonadotropin (hCG) and phenobarbital on the reproductive performance of fat-tailed Ghezel ewes. Small Rumin Res 30(3): 157-161. https://doi.org/10.1016/S0921-4488(98)00099-6
There are 26 citations in total.

Details

Primary Language Turkish
Subjects Veterinary Surgery
Journal Section Research Article
Authors

Abdurrahman Takcı 0000-0002-0569-7957

Publication Date August 15, 2019
Published in Issue Year 2019Volume: 1 Issue: 2

Cite

APA Takcı, A. (2019). Modifiye U-synch Protokolü ile Mevsim Dışı Senkronize Edilen Akkaraman Kangal Irkı Prepubertal Kuzu ve Primipar Koyunlara GnRH ve hCG Uygulamalarının Fertilite Üzerine Etkisi. Turkish Veterinary Journal, 1(2), 60-68.
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