Effects of Achillea millefolium Oil on HbA1c, Oxidative Stress, and DNA Damage in Streptozotocin-Induced Diabetic Rats

Kübra Güney Kaya; Selim Sekkin

doi:10.51755/turkvetj.1917682

TR EN

Achillea millefolium Yağının Streptozotosin ile İndüklenen Diyabetik Sıçanlarda Hba1c, Oksidatif Stres ve DNA Hasarı Üzerine Etkileri

Abstract

Diabetes mellitus, kalıcı hiperglisemi, oksidatif stres ve genomik instabilite ile karakterize edilmektedir. Achillea millefolium’un in vitro koşullarda antioksidan ve antidiyabetik özellikler gösterdiği bilinmekle birlikte, şiddetli insülin eksikliği koşullarındaki etkinliği henüz net değildir. Bu çalışmada, streptozotosin (STZ) ile indüklenen diyabetik sıçan modelinde Achillea millefolium yağının glisemik kontrol, hepatik oksidatif stres ve DNA hasarı üzerindeki etkileri değerlendirilmiştir. Toplam 35 erkek Wistar sıçan; kontrol, kontrol+civanperçemi, diyabetik ve diyabetik+civanperçemi gruplarına ayrılmıştır. Diyabet, tek doz intraperitoneal STZ (50 mg/kg) enjeksiyonu ile oluşturulmuş ve civanperçemi yağı (400 mg/kg/gün) 28 gün boyunca oral olarak uygulanmıştır. STZ ile oluşturulan diyabet; belirgin hiperglisemi, vücut ağırlığında azalma, hepatik malondialdehit düzeylerinde artış, glutatyon düzeylerinde azalma, süperoksit dismutaz aktivitesinde düşüş ve DNA hasarında artış ile sonuçlanmıştır (P<0.001). Civanperçemi yağı, diyabetik sıçanlarda glikozillenmiş hemoglobin (HbA1c) düzeylerini anlamlı şekilde azaltmıştır (P<0.001); ancak açlık plazma glukozu, antioksidan parametreler ve DNA hasarı göstergeleri üzerinde anlamlı bir etki göstermemiştir. Ayrıca, hepatik lipid peroksidasyonunun diyabetik+civanperçemi grubunda daha da arttığı belirlenmiştir. Elde edilen bulgular, Achillea millefolium yağının uzun dönem glisemik kontrol üzerinde sınırlı bir etkiye sahip olduğunu ve şiddetli insülin eksikliği koşullarında oksidatif stres ile genomik instabiliteyi azaltmada etkili olmadığını göstermektedir. Diyabetteki potansiyel rolünün daha iyi anlaşılabilmesi için doz optimizasyonu, fitokimyasal karakterizasyon ve etki mekanizmalarına yönelik ileri çalışmalara ihtiyaç vardır.

Keywords

Supporting Institution

Aydın Adnan Menderes Üniversitesi

Project Number

ADÜ-BAP VTF-17036

Ethical Statement

Bu çalışma, Aydın Adnan Menderes Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi (BAP Proje No: VTF-17036) tarafından desteklenmiştir.

Thanks

Bu çalışma, Aydın Adnan Menderes Üniversitesi’nde yürütülen bir yüksek lisans tezinden türetilmiştir. Yazarlar, Aydın Adnan Menderes Üniversitesi Tıp Fakültesi Deney Hayvanları Üretim ve Araştırma Laboratuvarı’na teşekkür eder.

Effects of Achillea millefolium Oil on HbA1c, Oxidative Stress, and DNA Damage in Streptozotocin-Induced Diabetic Rats

Abstract

Diabetes mellitus is characterized by persistent hyperglycemia, oxidative stress, and genomic instability. Although Achillea millefolium exhibits antioxidant and antidiabetic properties in vitro, its efficacy under conditions of severe insulin deficiency remains unclear. This study evaluated the effects of Achillea millefolium oil on glycemic control, hepatic oxidative stress, and DNA damage in a streptozotocin (STZ)-induced diabetic rat model. Thirty-five male Wistar rats were allocated to control, control + yarrow, diabetic, and diabetic + yarrow groups. Diabetes was induced by a single intraperitoneal injection of STZ (50 mg/kg), and yarrow oil (400 mg/kg/day) was administered orally for 28 days. STZ-induced diabetes resulted in marked hyperglycemia weight loss, increased hepatic malondialdehyde levels, reduced glutathione concentrations, decreased superoxide dismutase activity, and elevated DNA damage (P<0.001). Yarrow oil significantly reduced glycated hemoglobin (HbA1c) levels in diabetic rats (P<0.001); however, it did not improve fasting plasma glucose, antioxidant parameters, or DNA damage indices. Notably, hepatic lipid peroxidation was further increased in the diabetic+yarrow group. These findings indicate that Achillea millefolium oil exerts a limited effect on long-term glycemic control and does not mitigate oxidative stress or genomic instability under severe insulin-deficient conditions. Further studies focusing on dose optimization, phytochemical characterization, and mechanistic pathways are warranted.

Keywords

Supporting Institution

Aydın Adnan Menderes University

Project Number

ADÜ-BAP VTF-17036

Ethical Statement

This research was supported by the Scientific Research Projects Coordination Unit (BAP Project No. VTF-17036) of Aydın Adnan Menderes University.

Thanks

This study was derived from a master’s thesis conducted at Aydın Adnan Menderes University. The authors thank the Experimental Animals Production and Research Laboratory, Faculty of Medicine, Aydın Adnan Menderes University, Aydın, Türkiye.

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Details

Primary Language

English

Subjects

Veterinary Pharmacology

Journal Section

Research Article

Authors

Kübra Güney Kaya ^*
0000-0003-4830-2465
Türkiye

Selim Sekkin
0000-0002-3795-5375
Türkiye

Publication Date

June 30, 2026

Submission Date

March 27, 2026

Acceptance Date

May 14, 2026

Published in Issue

Year 2026 Volume: 8 Number: 1

DOI

https://doi.org/10.51755/turkvetj.1917682

IZ

https://izlik.org/JA75WH23PF

Cite

RIS / Bibtex

APA

Güney Kaya, K., & Sekkin, S. (2026). Effects of Achillea millefolium Oil on HbA1c, Oxidative Stress, and DNA Damage in Streptozotocin-Induced Diabetic Rats. Turkish Veterinary Journal, 8(1), 7-13. https://doi.org/10.51755/turkvetj.1917682

AMA

1.Güney Kaya K, Sekkin S. Effects of Achillea millefolium Oil on HbA1c, Oxidative Stress, and DNA Damage in Streptozotocin-Induced Diabetic Rats. Turk Vet J. 2026;8(1):7-13. doi:10.51755/turkvetj.1917682

Chicago

Güney Kaya, Kübra, and Selim Sekkin. 2026. “Effects of Achillea Millefolium Oil on HbA1c, Oxidative Stress, and DNA Damage in Streptozotocin-Induced Diabetic Rats”. Turkish Veterinary Journal 8 (1): 7-13. https://doi.org/10.51755/turkvetj.1917682.

EndNote

Güney Kaya K, Sekkin S (June 1, 2026) Effects of Achillea millefolium Oil on HbA1c, Oxidative Stress, and DNA Damage in Streptozotocin-Induced Diabetic Rats. Turkish Veterinary Journal 8 1 7–13.

IEEE

[1]K. Güney Kaya and S. Sekkin, “Effects of Achillea millefolium Oil on HbA1c, Oxidative Stress, and DNA Damage in Streptozotocin-Induced Diabetic Rats”, Turk Vet J, vol. 8, no. 1, pp. 7–13, June 2026, doi: 10.51755/turkvetj.1917682.

ISNAD

Güney Kaya, Kübra - Sekkin, Selim. “Effects of Achillea Millefolium Oil on HbA1c, Oxidative Stress, and DNA Damage in Streptozotocin-Induced Diabetic Rats”. Turkish Veterinary Journal 8/1 (June 1, 2026): 7-13. https://doi.org/10.51755/turkvetj.1917682.

JAMA

1.Güney Kaya K, Sekkin S. Effects of Achillea millefolium Oil on HbA1c, Oxidative Stress, and DNA Damage in Streptozotocin-Induced Diabetic Rats. Turk Vet J. 2026;8:7–13.

MLA

Güney Kaya, Kübra, and Selim Sekkin. “Effects of Achillea Millefolium Oil on HbA1c, Oxidative Stress, and DNA Damage in Streptozotocin-Induced Diabetic Rats”. Turkish Veterinary Journal, vol. 8, no. 1, June 2026, pp. 7-13, doi:10.51755/turkvetj.1917682.

Vancouver

1.Kübra Güney Kaya, Selim Sekkin. Effects of Achillea millefolium Oil on HbA1c, Oxidative Stress, and DNA Damage in Streptozotocin-Induced Diabetic Rats. Turk Vet J. 2026 Jun. 1;8(1):7-13. doi:10.51755/turkvetj.1917682