Stereolojical Investigation of Medulla Oblongata Volume in Turkeys and Guinea Fowls

Veysel Delibaş; Gamze Çakmak; Burçin Çelikezen; Mahmut Yardımcı

doi:10.51755/turkvetj.1807307

TR EN

Hindilerde ve Beç Tavuklarında Medulla Oblongata’nın Hacim Değerlerinin Stereolojik Olarak İncelenmesi

Öz

Bu çalışmanın amacı, beç tavukları (Numida meleagris) ve hindilerde (Meleagris gallopavo) medulla oblongata’nın morfometrik ve volumetrik özelliklerini stereolojik yöntemler ile karşılaştırmalı olarak incelemektir. Çalışmada, 8 aylık sağlıklı dişi hindiler ve beç tavukları kullanılmış, beyinler formaldehit çözeltisinde sabitlenmiş ve medulla oblongata dikkatlice dissekte edilmiştir. Medulla oblongata’nın uzunluk, çap ve ağırlık ölçümleri alınmış, 5 µm kalınlığındaki H&E ile boyanan kesitler üzerinden Cavalieri prensibi kullanılarak toplam hacim, medulla hacmi ve korteks hacmi hesaplanmıştır. Ayrıca medulla/korteks, korteks/toplam hacim ve medulla/toplam hacim oranları da değerlendirilmiştir. Sonuçlar, beç tavuklarında medulla oblongata ağırlığı ile medulla ve toplam hacim değerleri arasında güçlü pozitif korelasyon olduğunu göstermiştir. Özellikle medulla hacminin toplam hacme etkisi, korteks hacminin etkisinden daha belirgin bulunmuştur. Hindilerde medulla/korteks ve medulla/toplam hacim oranları hindilerden daha yüksek bulunurken, hindilerde korteks hacmi toplam hacme daha fazla katkı sağlamıştır. Ayrıca hindilerde medulla oblongata’nın ağırlığı ve uzunluğu, korteks hacmi ile pozitif ilişki gösterirken, medulla hacmi bu parametrelerden bağımsızdır. Morphometrik ölçümler ve stereolojik veriler arasındaki ilişkiler, medulla oblongata’nın yapısal gelişimini ve hacimsel büyümesini anlamada değerli bilgiler sunmuştur. Bu çalışmada elde edilen veriler, medulla oblongata’nın volumetrik ve morfolojik gelişimi, nöroanatomi ve karşılaştırmalı anatomi alanlarında güvenilir bir referans oluşturmakta; aynı zamanda hayvansal fizyoloji, biyometri, evrimsel biyoloji ve tarımsal üretim açısından da önemli uygulamalara işaret etmektedir. Stereolojik yöntemlerin medulla oblongata gibi küçük ve hassas dokuların analizinde güvenilir ve doğru sonuçlar sağladığı gösterilmiştir.

Anahtar Kelimeler

Stereolojical Investigation of Medulla Oblongata Volume in Turkeys and Guinea Fowls

Abstract

The present study aimed to investigate the morphometric and volumetric characteristics of the medulla oblongata in guinea fowls (Numida meleagris) and turkeys (Meleagris gallopavo) using stereological methods in a comparative manner. Eight-month-old healthy female turkeys and guinea fowls were used. Brains were fixed in formaldehyde, and the medulla oblongata was carefully dissected. Morphometric measurements including length, diameter, and weight were recorded. Using 5 µm-thick Hematoxylin-Eosin (H&E) stained sections, total volume, medulla volume, and cortex volume were estimated according to the Cavalieri principle, and ratios such as medulla/cortex, cortex/total volume, and medulla/total volume were calculated. Results indicated that in guinea fowls, medulla oblongata weight was positively correlated with medulla and total volumes, with medulla volume exerting a stronger effect on total volume than cortex volume. Medulla/cortex and medulla/total volume ratios were higher in guinea fowls than in turkeys, whereas in turkeys, cortex volume contributed more to total volume. In turkeys, medulla oblongata weight and length were positively associated with cortex volume but not with medulla volume. These findings highlight that morphometric parameters are closely linked to volumetric characteristics, providing valuable insights into the structural development and volumetric growth of the medulla oblongata. The study provides reliable reference data for veterinary anatomy, neuroanatomy, comparative anatomy, and physiology. It demonstrates the applicability and accuracy of stereological methods for analyzing small and delicate structures such as the medulla oblongata. Furthermore, the results have implications for biometry, evolutionary biology, and animal production, offering a foundation for future multidisciplinary research on brain morphology and development.

Keywords

Supporting Institution

Scientific Research Projects Coordination Unit, Van Yuzuncu Yil University

Project Number

TUY-2025-12391

Thanks

We want to thank to Scientific Research Projects Coordination Unit, Van Yuzuncu Yil University

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Details

Primary Language

English

Subjects

Veterinary Anatomy and Physiology

Journal Section

Research Article

Authors

Veysel Delibaş
0000-0003-1781-7511
Türkiye

Gamze Çakmak ^*
0000-0002-3970-3040
Türkiye

Burçin Çelikezen
0000-0003-3097-9814
Türkiye

Mahmut Yardımcı
0000-0002-1542-3968
Türkiye

Publication Date

December 29, 2025

Submission Date

October 20, 2025

Acceptance Date

November 17, 2025

Published in Issue

Year 2025 Volume: 7 Number: 2

DOI

https://doi.org/10.51755/turkvetj.1807307

IZ

https://izlik.org/JA24JF66CN

Cite

RIS / Bibtex

APA

Delibaş, V., Çakmak, G., Çelikezen, B., & Yardımcı, M. (2025). Stereolojical Investigation of Medulla Oblongata Volume in Turkeys and Guinea Fowls. Turkish Veterinary Journal, 7(2), 48-55. https://doi.org/10.51755/turkvetj.1807307