Investigation of Paraoxonase and Ceruloplasmin Concentrations in Hair Goats Grazing at Different Heights

Yeliz Kaya Kartal; Serdal Kurt; Funda Eşki; Seçkin Salar; İdil Baştan; Ayhan Baştan; Tevhide Sel

doi:10.51755/turkvetj.1691492

TR EN

"Farklı Yüksekliklerde Otlayan Kıl Keçilerinde Paraoksonaz ve Serüloplazmin Konsantrasyonlarının Araştırılması"

Öz

Bu çalışmanın amacı, farklı rakımlarda otlatılan kıl keçilerinde Paraoksonaz ve Serüloplazmin konsantrasyonlarındaki değişiklikleri ortaya koymaktır. Çalışmaya Adana'nın farklı bölgelerinde yaşayan 80 kıl keçisi dâhil edilmiş ve bu keçiler dört gruba ayrılmıştır. Birinci grup deniz seviyesinden 10-99 metre yükseklikte, ikinci grup 240 metre yükseklikte, üçüncü grup 750-800 metre yükseklikte ve dördüncü grup ise 1200-1500 metre yükseklikte otlatılmıştır. Yaz aylarında jugular venden alınan kan örnekleri kırmızı kapaklı tüplere alınmıştır. Paraoksonaz ve Serüloplazmin analizleri spektrofotometrik yöntemle gerçekleştirilmiştir. Paraoksonaz sonuçları, 240 metre (1113,91±84,57 U/mL) ve 750-800 metre (974,27±69,58 U/mL) gruplarında, 10-99 metre grubuna (651,72±52,93 U/mL; p<0,01**) göre istatistiksel olarak anlamlı şekilde yüksek bulunmuştur. Serüloplazmin sonuçlarında ise 10-99 metre (10,15±0,63 U/mL) ile 750-800 metre (7,13±0,46 U/mL) grubu arasında, ayrıca 240 metre (9,97±0,86 U/mL) ile 750-800 metre (7,13±0,46 U/mL) grubu arasında istatistiksel olarak anlamlı fark saptanmıştır (p<0,05*). Yapılan çalışmalar, yüksek rakımda yaşamaya alışık popülasyonlarda antioksidan parametrelerin, sonradan yüksek rakıma taşınanlara göre daha düşük olabileceğini göstermektedir. Normalde deniz seviyesine yakın yerlerde yaşayan ve sonradan yüksek rakımlara çıkan bireylerde, hipoksiye bağlı olarak vücut savunma sistemleri aktive olmakta ve buna bağlı olarak antioksidan enzim düzeylerinde artış beklenmektedir. 10-99 metre rakımda otlatılan kıl keçilerinde en yüksek serüloplazmin konsantrasyonunun gözlemlenmesinin, bu seviyelerde sıcaklığın daha yüksek olmasına ve buna bağlı olarak ısı stresinin akut faz proteinlerini artırmasına bağlanabileceği; benzer şekilde, ısı stresinin paraoksonaz enzim aktivitesinde azalmaya neden olduğu düşünülmektedir.

Anahtar Kelimeler

Investigation of Paraoxonase and Ceruloplasmin Concentrations in Hair Goats Grazing at Different Heights

Abstract

The aim of this study is to reveal the changes in Paraoxonase and Ceruloplasmin concentrations in hair goats grazed at different altitudes. 80 hair goats living in different regions in Adana were included in the study and divided into four groups. The first group was grazed at an altitude of 10-99 meters above sea level, the second group was grazed at an altitude of 240 meters, the third group was grazed at an altitude of 750-800 meters and the fourth group was grazed at an altitude of 1200-1500 meters. Blood samples collected from the jugular vein during the summer months were taken into red-capped tubes. Paraoxonase and Ceruloplasmin analyzes were analyzed by spectrophotometric method. The results of paraoxonase in 240 meter (1113.91±84.57 U/mL) and 750-800 meter (974.27±69.58 U/mL) were found statistically higher than 10-99 meter group (651.72±52.93 U/mL; p<0.01**). And the results of ceruloplasmin were found statistically different between 10-99 meter (10.15±0.63 U/mL) and 750-800 meter (7.13±0.46 U/mL), the 240 meter group (9.97±0.86 U/mL) and 750-800 meter group (7.13±0.46 U/mL) showed statistically significant difference too (p<0.05*). Studies have shown that antioxidant parameters of populations accustomed to living at high altitudes may be lower than those later moved to higher altitudes. In populations that normally live close to sea level and move to high altitudes later, their body defense systems are activated due to hypoxia, and antioxidant enzymes are expected to increase. It is thought that the reason why the highest ceruloplasmin concentration was observed in hair goats grazed at 10-99 meters may be that the temperature is higher at those levels and heat stress increases the acute phase proteins and likewise heat stress causes to a decrease in paraoxonase enzyme activity.

Keywords

Supporting Institution

Cukurova University, Local Ethics Committee of Ceyhan Faculty of Veterinary Medicine

Ethical Statement

This study was carried out with the permission of the Cukurova University, Local Ethics Committee of Ceyhan Faculty of Veterinary Medicine (Decision Date and Number: 12.12.2018 and 1/11).

References

Askew, E. W. (2002). Work at high altitude and oxidative stress: antioxidant nutrients. Toxic, 180, 107-119. DOI: 10.1016/s0300-483x(02)00385-2
Aviram, M. & Rosenblat, M. (2008). Paraoxonases (PON1, PON2, PON3) analyses in vitro and in vivo in relation to cardiovascular diseases. Methods Mol Biol, 477, 259-276.DOI: 10.1007/978-1-60327-517-020
Bakonyi, T. & Radak, Z. (2004). High altitude and free radicals. J Sport Sci Med, 3(2), 64-69.
Banerjee, D., Upadhyay, R. C., Chaudhary, U. B., Kumar, R., Singh, S., Ashutosh, G. J. M., Polley, S., Mukherjee, A., Das, T. K. & De, S. (2015). Seasonal variations in physio-biochemical profiles of Indian goats in the paradigm of hot and cold climate. Biol Rhythm Res, 46, 221-236. DOI: 10.1007/s12192-013-0469-0
Cabrera de Leon, A., Perez, M. C. R., Gonzalez, D. A., Díaz, B.B., Coello, S. D., Hernández, A. G. & Aguirre-Jaime,A.(2011). Hemodynamics and Metabolism at Lowversus Moderate Altitudes. High alt med biol, 12, 179-186.DOI: 10.1089/ham.2010.1078
Ceron, J. & Martinez-Subiela, S. (2004). An automated spectrophotometric method for measuring canine ceruloplasmin in serum. Vet Res, 35, 671–679.
Curzon, G. & Vallet, L. (1960). The Purification of Human Ceruloplasmin. Biochem J, 74, 279-287. DOI: 10.1051/vetres:2004046
Demirpence, O., Sevim, B., Yildirim, M., Ayan Nurlu, N., Mert, D. & Evliyaoğlu, O. (2014). Serum paraoxonase, TAS, TOS and ceruloplasmin in brucellosis. Int J Clin Exp Med, 7, 1592-1597.

Er, C. & Ok, M. (2020). Determination The Levels of Some Biochemical Parameters in Female Hair Goats in Cukurova Region. Kocatepe Vet J, 13, 25-29. DOI: 10.30607/kvj.623064
Field, A. (2013). Discovering Statistics Using Ibm Spss Statistics. 4th edn. Sage Publication Ltd.
Fleck, A. (1989). Clinical and nutritional aspects of changes in acute phase proteins during inflammation. Proc Nutr Soc, 48, 347–354. DOI: 10.1079/pns19890050
Gocmen, A. Y., Sahin, E., Semiz, E. & Gümuşlü, S. (2008). Is elevated serum ceruloplasmin level associated with increased risk of coronary artery disease? Can J Cardiol, 24, 209-212. DOI: 10.1016/s0828-282x(08)70586-5
Gungor, I., Alkoyak, K., Oz, S. & Koncagül, S. (2021). Growth, survival rate, and some reproductive characteristics of Hair goat under breeder conditions in Kahramanmaras Province. Turkish J of Vet and Anim Sci, 45, 1022-1029. DOI: 10.3906/vet-2104-51
Gunlu, A. & Alasahan, S. (2010). Evaluations on the Future of Goat Breeding in Turkey. Vet Hek Der Derg, 81, 15-20.
Halliwell, B., Murcia, M. A., Chirico, S. & Aruoma, O. I. (1995). Free radicals and antioxidants in food and in vivo: what they do and how they work. Crit Rev Food Sci Nutr, 35, 7-20. DOI: 10.1080/10408399509527682
Hirschler, V., Martin, M., Molinari, C., Botta, E., Tetzlaff, W. F. & Brites, F. (2019). Activity of Lipoprotein Associated Enzymes in Indigenous Children Living at Different Altitudes. Archives of Med Res, 50, 98-104. DOI: 10.1016/j.arcmed.2019.07.001
Hirschler, V., Martin, M., Oestreicher, K., Molinari, C., Tetzlaff, W., Botta, E., Boero, L., Brites, F. (2018). Activity of the antioxidant enzyme paraoxonase in Argentinean children living at high altitude. Redox Rep, 23, 35-40. DOI: 10.1080/13510002.2017.1370783
James, R. W. & Deakin, S. P. (2004). The importance of high-density lipoproteins for paraoxonase-1 secretion, stability, and activity. Free Radic Biol Med, 12, 1986–1994. DOI: 10.1016/j.freeradbiomed.2004.08.012
Kartal, Y. K., Kurt, S., Eski, F., Salar, S., Kismali, G., Baştan, A.& Sel, T. (2021). Alteration of paraoxonase,ceruloplasmin and immunoglobulin G levels in hairgoats at different ages. J Adv Vet Bio Sci Tech, 7, 7-12. DOI: 10.31797/vetbio.984872
Kaymakci, M., Taskin, T., Kosum, N., et al (2004). Organik süt üretimini Türkiye’de geliştirme olanakları. I. International Organic Animal Production and Food Safety Congress. Kusadası, 28 April-1 May 2004, 358-370.
Koyuncu, M. & Taskin, T. (2016). Organic Sheep and Goat Production. Hay Üret, 51, 56-62.
Kurt, S., Eski, F., Salar, S., Sel, T. & Baştan, A. (2021). The effect of heat stress on total oxidant capacity in hair goats. J of the Turkish Vet Med Soc, 92, 24-30. DOI: 10.33188/vetheder.771696 Mohammed, S. A., Razzaque, M. A., Omar, A. E., Albert, S. & Al-Gallaf, W. M. (2016). Biochemical and hematological profile of different breeds of goat maintained under intensive production system. African J of Biotech, 15, 1253-1257. DOI: 10.5897/AJB2016.15362
Muratsubaki, H., Enomoto, K., Ichijoh, Y. & Yamamoto, Y. (2003). Hypertriglyceridemia associated with decreased post-heparin plasma hepatic triglyceride lipase activity in hypoxic rats. Arch Physiol Biochem, 111, 449-454. DOI: 10.3109/13813450312331342319
Sinha, S., Ray, U. S., Saha, M., Singh, S. N. & Tomar, O. S. (2009c). Antioxidant and redox status after maximal aerobic exercise at high altitude in acclimatized lowlanders and native highlanders. Eur J Appl Physiol, 106, 807–814. DOI: 10.1007/s00421-009-1082-x
Sinha, S., Ray, U. S., Tomar, O. S. & Singh, S. N. (2009a). Different adaptation patterns of antioxidant system in natives and sojourners at high altitude. Respir Physiol Neurobiol, 167, 255-260. DOI: 10.1016/j.resp.2009.05.003
Sinha, S., Singh, S. N. & Ray, U. S. (2009b). Total Antioxidant Status at High Altitude in Lowlanders and Native Highlanders: Role of Uric Acid. High Alt Med Biol, 10, 269-274. DOI: 10.1089/ham.2008.1082
Yildirim, A., Sahin, Y. N., Turhan, H., Şen, İ. & Kaplan, İ. (2008). Dağcılarda yüksek rakıma maruz kalmanın bazı serum akut faz proteinleri üzerine etkisi. Fırat Tıp Derg, 13, 239-242.
Yilmaz, O., Kor, A., Ertugrul, M. & Wilson, R. T. (2012). The domestic livestock resources of Tukey: goat breed and types and their conservation status. Anim Gen Resourc, 51, 105-116. DOI: 10.1294/jes.23.47

Details

Primary Language

English

Subjects

Veterinary Biochemistry

Journal Section

Research Article

Authors

Yeliz Kaya Kartal ^*
0000-0002-3661-5504
Türkiye

Serdal Kurt
0000-0002-0191-3245
Türkiye

Funda Eşki
0000-0002-9242-9271
Türkiye

Seçkin Salar
0000-0001-9303-6253
Türkiye

İdil Baştan
0000-0003-2588-4036
Türkiye

Ayhan Baştan
0000-0001-8291-1147
Türkiye

Tevhide Sel
0000-0002-9753-779X
Türkiye

Early Pub Date

June 30, 2025

Publication Date

June 30, 2025

Submission Date

May 4, 2025

Acceptance Date

June 25, 2025

Published in Issue

Year 2025 Volume: 7 Number: 1

DOI

https://doi.org/10.51755/turkvetj.1691492

IZ

https://izlik.org/JA77YC98UH

Cite

RIS / Bibtex

APA

Kaya Kartal, Y., Kurt, S., Eşki, F., Salar, S., Baştan, İ., Baştan, A., & Sel, T. (2025). Investigation of Paraoxonase and Ceruloplasmin Concentrations in Hair Goats Grazing at Different Heights. Turkish Veterinary Journal, 7(1), 6-10. https://doi.org/10.51755/turkvetj.1691492