Impact of Tebuconazole Fungicide on Drone Semen Quality

Abdulkadir Kaya; Ruhi Kabakçı

doi:10.51755/turkvetj.1577784

TR EN

Bir Fungusit Olan Tebukonazol’ün Arı Sperma Kalitesine Etkisi

Öz

Tebukonazol (TEB) özellikle meyve ağacı yetiştiriciliğinde yaygın olarak kullanılan triazol grubu bir fungisittir. Çoğu türde hücresel ve hormonal mekanizmaları bozarak üreme sistemi üzerinde olumsuz etkileri vardır. Meyve ağaçlarında TEB kalıntılarının varlığı birçok çalışma ile kanıtlanmıştır. Ancak, erkek arıların üreme parametreleri üzerindeki etkisine ilişkin bir çalışmaya rastlanılmamıştır. Bal arıları üreme sistemleri açısından risk altında kabul edilmektedir çünkü büyük olasılıkla bu kimyasalı nektar ve polen yoluyla koloniye taşımaktadırlar. Bundan dolayı, bu çalışmada TEB' in bal arısının üreme sisteminde önemli bir unsur olan erkek arı sperm kalitesi üzerindeki potansiyel toksik etkilerinin doza ve zamana bağlı olarak araştırılması amaçlandı. Her biri 1.0- 1.5 x 108/ml spermatozoa içeren beş farklı tüpteki bal arısı sperması 24 saat boyunca sırasıyla 0, 1, 10, 100 ve 1000 µM TEB' e maruz bırakıldı. Daha sonra sperma 0 ve 24. saatlerde motilite (MOT), plazma membran bütünlüğü (PMB) ve mitokondriyal membran potansiyeli (MMP) açısından analiz edildi. Bu çalışmanın bulguları, en yüksek TEB konsantrasyonunun (1000 µM) arı spermasının MOT ve PMB değerlerini 0. saatte bile diğer konsantrasyonlara kıyasla önemli ölçüde azalttığını (p≤0,05) ortaya koydu. 24 saatlik inkübasyonun ardından, 1000 µM TEB'e maruz kalan grupta MOT, PMB ve MMP değerleri diğer gruplara kıyasla önemli ölçüde daha düşüktü (p≤0,05). Öte yandan, hem 0. hem de 24. saatte TEB' in 0-100 µM arasındaki düşük dozları çalışmada incelenen hiçbir parametreyi anlamlı bir şekilde değiştirmedi (p>0,05). Sonuç olarak, sadece en yüksek TEB dozunda gözlenmesine rağmen, sonuçlarımız TEB' in erkek arı sperması üzerinde zararlı bir etkisi olduğunu gösterdi. Ayrıca, gelecekte in vitro ve in vivo araştırmalarla desteklenen daha kapsamlı çalışmaların yapılması faydalı olacaktır.

Anahtar Kelimeler

Impact of Tebuconazole Fungicide on Drone Semen Quality

Abstract

Tebuconazole (TEB) is a widely used fungicide of the triazole group, especially in fruit tree cultivation. It has adverse effects on the reproductive system by disrupting cellular and hormonal mechanisms in most species. Lots of studies are proven the presence of TEB’s residues on fruit trees. However, no study has been found on its effect on the reproductive parameters of drones. Honey bees are considered at risk in terms of reproductive systems since they most probably transport this chemical to the colony through nectar and pollen. Therefore, it was aimed to investigate to potential toxic effects of TEB on drone semen quality, a crucial element in reproductive system of honey bee in dose and time dependent manner. Honey bee semen in five different tubes, each containing 1.0 – 1.5 x 108/ml spermatozoa, were exposed to 0, 1, 10, 100, and 1000 µM of TEB, respectively for 24 h. Afterward, semen were analyzed for motility (MOT), plasma membrane integrity (PMI), and mitochondrial membrane potential (MMP) at 0 and 24 hours. The findings of this study revealed that highest concentration of TEB (1000 µM) significantly reduced (p≤0.05) MOT and PMI of semen compared to other concentrations even at 0 h. Following 24 h incubation, MOT, PMI and MMP values of groups exposed to 1000 µM TEB significantly lower (p≤0.05) than other groups. On the other hand, lower concentrations of TEB between 0-100 µM did not significantly change any parameters evaluated in this study at both 0 and 24 h (p>0.05). In conclusion, although it is only observed at the highest dose of TEB, our results showed that TEB has a detrimental effect on drone semen. Furthermore, it would be useful to conduct more comprehensive studies supported by in vitro and in vivo research in the future.

Keywords

References

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Details

Primary Language

English

Subjects

Veterinary Sciences (Other)

Journal Section

Research Article

Authors

Abdulkadir Kaya ^*
0000-0001-7903-4358
Türkiye

Ruhi Kabakçı
0000-0001-9131-0933
Türkiye

Early Pub Date

December 28, 2024

Publication Date

December 31, 2024

Submission Date

November 1, 2024

Acceptance Date

December 16, 2024

Published in Issue

Year 2024 Volume: 6 Number: 2

DOI

https://doi.org/10.51755/turkvetj.1577784

IZ

https://izlik.org/JA73KE24FU

Cite

RIS / Bibtex

APA

Kaya, A., & Kabakçı, R. (2024). Impact of Tebuconazole Fungicide on Drone Semen Quality. Turkish Veterinary Journal, 6(2), 71-77. https://doi.org/10.51755/turkvetj.1577784

Cited By

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Journal of Apicultural Research

https://doi.org/10.1080/00218839.2025.2560703