Sıçanlarda Akut Kısıtlama ve Soğuk Kısıtlama Stres Tepkisinin Elektrokardiyografik Değerlendirmesi

Çağlasu Koç; Mehmet Ekici

doi:10.51755/turkvetj.1244382

EN TR

Electrocardiographic Evaluation of Acute Restraint and Cold Restraint Stress Response in Rats

Abstract

In this study, we investigated the effects of acute restraint stress and cold restraint stress on electrocardiographic (ECG) parameters in rats. A total of 18 male Wistar albino rats aged 8-10 weeks weighing 180-220 g were divided into three equal groups (n=6/group). No stressors were administered to the rats in the control group. The rats in the restraint group were kept in a restrainer for 2 hours. The rats in the cold restraint group were kept at +4°C for 2 hours in restrainer. ECG was recorded under ketamine and xylazine anesthesia in rats with the Televet II ECG device (Kruuse, Germany), and the Televet 100 program (version® 7.0.0, Kruuse, Heusenstamm, Germany) was used to analyze the leads II. There was no significant difference between the groups in the analysis of PR, QT, QRS intervals, and R-wave amplitude (p >0.05). However, the heart rate was significantly higher in the cold restraint group than in the control group (p <0.001) and restraint group (p <0.01). Conversely, the RR interval was significantly lower in the cold restraint group than in the control group (p <0.001) and restraint group (p <0.01). The QTc was increased in rats exposed to cold restraint stress compared to the control group (p <0.01) and restraint group (p <0.05). In conclusion, acute cold restraint stress may cause more pronounced ECG change than restraint stress.

Keywords

Sıçanlarda Akut Kısıtlama ve Soğuk Kısıtlama Stres Tepkisinin Elektrokardiyografik Değerlendirmesi

Öz

Bu çalışmada, akut kısıtlama stresi ve soğuk kısıtlama stresinin sıçanlarda elektrokardiyografik (EKG) parametreler üzerindeki etkilerini araştırıldı. Toplam 18 adet 8-10 haftalık, ağırlıkları 180-220 g olan erkek Wistar albino rat üç eşit gruba (n=6/grup) ayrıldı. Kontrol grubundaki ratlara stresör uygulanmadı. Kısıtlama grubundaki ratlar 2 saat kısıtlayıcıda tutuldu. Soğuk tutma grubundaki ratlar kısıtlayıcı içinde 2 saat +4°C'de tutuldu. Sıçanlarda Televet II EKG cihazı (Kruuse, Almanya) ile ketamin ve ksilazin anestezisi altında EKG kaydı yapıldı ve derivasyonların analizinde Televet 100 programı (versiyon® 7.0.0, Kruuse, Heusenstamm, Almanya) kullanıldı. PR, QT, QRS intervalleri ve R dalgası amplitüdü analizinde gruplar arasında anlamlı fark yoktu (p >0,05). Ancak, kalp atım hızı soğuk kısıtlama grubunda kontrol grubuna (p <0.001) ve kısıtlama grubuna (p <0.01) göre anlamlı derecede yüksekti. Tersine, RR aralığı soğuk kısıtlama grubunda kontrol grubuna (p <0.001) ve kısıtlama grubuna (p <0.01) göre anlamlı derecede düşüktü. QTc, soğuk kısıtlama stresine maruz bırakılan sıçanlarda, kontrol grubu (p <0.01) ve kısıtlama grubuna (p <0.05) göre arttı. Sonuç olarak, akut soğuk kısıtlama stresi, kısıtlama stresinden daha belirgin EKG değişikliğine neden olabilir.

Anahtar Kelimeler

Supporting Institution

TUBİTAK

Project Number

2209- A - Üniversite Öğrencileri Araştırma Projeleri

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Details

Primary Language

Turkish

Subjects

Veterinary Anatomy and Physiology

Journal Section

Research Article

Authors

Çağlasu Koç
0000-0002-2925-6849
Türkiye

Mehmet Ekici ^*
0000-0002-2163-6214
Türkiye

Early Pub Date

July 28, 2023

Publication Date

July 31, 2023

Submission Date

January 30, 2023

Acceptance Date

May 3, 2023

Published in Issue

Year 2023 Volume: 5 Number: 1

DOI

https://doi.org/10.51755/turkvetj.1244382

IZ

https://izlik.org/JA33AB53LN

Cite

RIS / Bibtex

APA

Koç, Ç., & Ekici, M. (2023). Sıçanlarda Akut Kısıtlama ve Soğuk Kısıtlama Stres Tepkisinin Elektrokardiyografik Değerlendirmesi. Turkish Veterinary Journal, 5(1), 8-12. https://doi.org/10.51755/turkvetj.1244382