The Biotechnological Potential of Baculoviruses: From Insect Viruses to Biotechnology Workhorse

Remziye Özbek

doi:10.51755/turkvetj.1713887

TR EN

The Biotechnological Potential of Baculoviruses: From Insect Viruses to Biotechnology Workhorse

Öz

Baculoviruses are a significant group of arthropod viruses that are widely recognized for their potential as biological control agents against pests in agriculture and forestry. The Baculoviridae is a vast family of viruses that primarily infects various species within the Arthropoda phylum, particularly insects. Baculoviruses are widely used not only as biopesticides in agricultural applications but also as efficient tools for recombinant protein production. The Baculovirus Expression Vector System (BEVS) has been shown to be particularly effective for expressing complex or difficult-to-produce proteins in mammalian cells. Owing to its high expression capacity and post-translational modification capabilities, BEVS has been successfully employed in various biotechnological fields, including vaccine development, therapeutic protein production, and the synthesis of enzymes and antibodies. In this review, the BEVS technique, which is one of the significant areas of use of Baculoviruses, is discussed along with its advantages and several of its practical applications.

Anahtar Kelimeler

The Biotechnological Potential of Baculoviruses: From Insect Viruses to Biotechnology Workhorse

Abstract

Baculoviruses are a significant group of arthropod viruses that are widely recognized for their potential as biological control agents against pests in agriculture and forestry. The Baculoviridae is a vast family of viruses that primarily infects various species within the Arthropoda phylum, particularly insects. Baculoviruses are widely used not only as biopesticides in agricultural applications but also as efficient tools for recombinant protein production. The Baculovirus Expression Vector System (BEVS) has been shown to be particularly effective for expressing complex or difficult-to-produce proteins in mammalian cells. Owing to its high expression capacity and post-translational modification capabilities, BEVS has been successfully employed in various biotechnological fields, including vaccine development, therapeutic protein production, and the synthesis of enzymes and antibodies. In this review, the BEVS technique, which is one of the significant areas of use of Baculoviruses, is discussed along with its advantages and several of its practical applications.

Keywords

References

Adeniyi, A. A., & Lua, L. H. (2020). Protein Expression in the Baculovirus-Insect Cell Expression System. In: J. Gerrard, & L. Domigan (Eds.), Protein Nanotechnology: Methods in Molecular Biology (pp. 17–37). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9869-2_2
Aoki, H., Sakoda, Y., Jukuroki, K., Takada, A., Kida, H., & Fukusho, A. (1999). Induction of antibodies in mice by a recombinant baculovirus expressing pseudorabies virus glycoprotein B in mammalian cells. Veterinary microbiology, 68(3-4), 197–207. https://doi.org/10.1016/s0378-1135(99)00110-8
Balani, P., Boulaire, J., Zhao, Y., Zeng, J., Lin, J., & Wang, S. (2009). High mobility group box2 promoter-controlled suicide gene expression enables targeted glioblastoma treatment. Molecular therapy : the journal of the American Society of Gene Therapy, 17(6), 1003–1011. https://doi.org/10.1038/mt.2009.22
Blanco, J. C., Boukhvalova, M. S., Pletneva, L. M., Shirey, K. A., & Vogel, S. N. (2014). A recombinant anchorless respiratory syncytial virus (RSV) fusion (F) protein/monophosphoryl lipid A (MPL) vaccine protects against RSV-induced replication and lung pathology. Vaccine, 32(13), 1495–1500. https://doi.org/10.1016/j.vaccine.2013.11.032
Blazevic, V., Malm, M., Arinobu, D., Lappalainen, S., & Vesikari, T. (2016). Rotavirus capsid VP6 protein acts as an adjuvant in vivo for norovirus virus-like particles in a combination vaccine. Human vaccines & immunotherapeutics, 12(3), 740–748. https://doi.org/10.1080/21645515.2015.1099772
Bruder, M. R., & Aucoin, M. G. (2022). Utility of Alternative Promoters for Foreign Gene Expression Using the Baculovirus Expression Vector System. Viruses, 14(12), 2670. https://doi.org/10.3390/v14122670
Caillava, A. J., Alfonso, V., Tejerina Cibello, M., Demaria, M.A., Coria, L. M., Cassataro, J., Taboga, O. A., & Alvarez, D. E. (2024). A vaccine candidate based on baculovirus displaying chikungunya virus E1-E2 envelope confers protection against challenge in mice.Journal of virology, 98(11), e0101724.https://doi.org/10.1128/jvi.01017-24
Chambers, A. C., Aksular, M., Graves, L. P., Irons, S. L., Possee, R. D., & King, L. A. (2018). Overview of the baculovirus expression system. Current Protocols in Protein Science, 91, 5.4.1–5.4.6. doi: 10.1002/cpps.47

Clem, R. J., & Passarelli, A. L. (2013). Baculoviruses: sophisticated pathogens of insects. PLoS pathogens, 9(11), e1003729. https://doi.org/10.1371/journal.ppat.1003729
Cory, J. S., & Bishop, D. H. (1997). Use of baculoviruses as biological insecticides. Molecular biotechnology, 7(3), 303–313. https://doi.org/10.1007/BF02740821
Dai, S., Zhang, T., Zhang, Y., Wang, H., & Deng, F. (2018). Zika Virus Baculovirus-Expressed Virus-Like Particles Induce Neutralizing Antibodies in Mice. Virologica Sinica, 33(3), 213–226. https://doi.org/10.1007/s12250-018-0030-5
Espíritu-Ramírez, P., Ortega-Balderas, N. Y., Sevilla-Tapia, L., Montiel-Martínez, A. G., Pastor-Flores, A. R., Palomares, L. A., & Torres-Vega, M. A. (2018). Gene Therapy for Treatment of Chronic Hyperammonemia in a Rat Model of Hepatic Encephalopathy. Annals of hepatology, 17(6), 1026–1034. https://doi.org/10.5604/01.3001.0012.7203
Fath-Goodin, A., Kroemer, J., Martin, S., Reeves, K., & Webb, B. A. (2006). Polydnavirus genes that enhance the baculovirus expression vector system. Advances in virus research, 68, 75–90. https://doi.org/10.1016/S0065-3527(06)68002-0
Felberbaum R. S. (2015). The baculovirus expression vector system: A commercial manufacturing platform for viral vaccines and gene therapy vectors. Biotechnology journal, 10(5), 702–714. https://doi.org/10.1002/biot.201400438
Feng, E., Luo, G., Wang, C., Liu, W., Yan, R., Bai, X., & Cheng, Y. (2025). Generation and Immunogenicity of Virus-like Particles Based on the Capsid Protein of a Chinese Epidemic Strain of Feline Panleukopenia Virus. Veterinary sciences, 12(5), 503. https://doi.org/10.3390/vetsci12050503
Fraser M. J. (1992). The baculovirus-infected insect cell as a eukaryotic gene expression system. Current topics in microbiology and immunology, 158, 131–172. https://doi.org/10.1007/978-3-642-75608-5_6
Garcia Fallit, M., Pidre, M. L., Asad, A. S., Peña Agudelo, J. A., Vera, M. B., Nicola Candia, A. J., Sagripanti, S. B., Pérez Kuper, M., Amorós Morales, L. C., Marchesini, A., Gonzalez, N., Caruso, C. M., Romanowski, V., Seilicovich, A., Videla-Richardson, G. A., Zanetti, F. A., & Candolfi, M. (2023). Evaluation of Baculoviruses as Gene Therapy Vectors for Brain Cancer. Viruses, 15(3), 608.https://doi.org/10.3390/v15030608
Ge, J., Liu, Y., Jin, L., Gao, D., Bai, C., & Ping, W. (2016). Construction of recombinant baculovirus vaccines for Newcastle disease virus and an assessment of their immunogenicity. Journal of biotechnology, 231, 201–211.https://doi.org/10.1016/j.jbiotec.2016.03.037
GenBank. (01.04.2024). Baculoviridae-91 complete genomes. http://www.ncbi.nlm.nih.gov/genomes/GenomesGroup$.cgi?taxid=10442.
Grzywacz, D. (2017). Basic and Applied Research. Microbial Control of Insect and Mite Pests, 27–46. https://doi.org/10.1016/B978-0-12-803527-6.00003-2
Haase, S., Ferrelli, L., Luis, M., & Romanowski, V. (2013). Genetic Engineering of Baculoviruses. In R. Victor (Ed.) Current Issues in Molecular Virology - Viral Genetics and Biotechnological Applications (pp. 80-111). InTech. http://dx.doi.org/10.5772/56976.
Harrison, R. L., Herniou, E. A., Jehle, J. A., Theilmann, D. A., Burand, J. P., Becnel, J. J., Krell, P. J., van Oers, M. M., Mowery, J. D., Bauchan, G. R., & Ictv Report Consortium (2018). ICTV Virus Taxonomy Profile: Baculoviridae. The Journal of general virology, 99(9), 1185–1186. https://doi.org/10.1099/jgv.0.001107 Heinimäki, S., Tamminen, K., Malm, M., Vesikari, T., & Blazevic, V. (2017). Live baculovirus acts as a strong B and T cell adjuvant for monomeric and oligomeric protein antigens. Virology, 511, 114–122. https://doi.org/10.1016/j.virol.2017.08.023
Herniou, E. A., & Jehle, J. A. (2007). Baculovirus phylogeny and evolution. Current drug targets, 8(10), 1043–1050. https://doi.org/10.2174/138945007782151306
Hitchman, R. B., Locanto, E., Possee, R. D., & King, L. A. (2011). Optimizing the baculovirus expression vector system. Methods (San Diego, Calif.), 55(1), 52–57. https://doi.org/10.1016/j.ymeth.2011.06.011
Hong, M., Li, T., Xue, W., Zhang, S., Cui, L., Wang, H., Zhang, Y., Zhou, L., Gu, Y., Xia, N., & Li, S. (2022). Genetic engineering of baculovirus-insect cell system to improve protein production. Frontiers in bioengineering and biotechnology, 10, 994743. https://doi.org/10.3389/fbioe.2022.994743
Hong, Q., Liu, J., Wei, Y., & Wei, X. (2023). Application of Baculovirus Expression Vector System (BEVS) in Vaccine Development. Vaccines, 11(7), 1218. https://doi.org/10.3390/vaccines11071218
Hu Y. C. (2006). Baculovirus vectors for gene therapy. Advances in virus research, 68, 287–320. https://doi.org/10.1016/S0065-3527(06)68008-1
Hu, B., Dong, W., Song, Y., Fan, Z., Cavadini, P., & Wang, F. (2025). Detection of a New Recombinant Rabbit Hemorrhagic Disease Virus 2 in China and Development of Virus-like Particle-Based Vaccine. Viruses, 17(5), 710. https://doi.org/10.3390/v17050710
Hu, J., Liang, Y., Hu, Z., Wang, X., Gu, M., Li, R., Ma, C., Liu, X., Hu, S., Chen, S., Peng, D., Jiao, X., & Liu, X. (2019). Recombinant baculovirus vaccine expressing hemagglutinin of H7N9 avian influenza virus confers full protection against lethal highly pathogenic H7N9 virus infection in chickens. Archives of virology, 164(3), 807–817. https://doi.org/10.1007/s00705-018-04142-4
Huo, X., Chen, Y., Zhu, J., & Wang, Y. (2023). Evolution, genetic recombination, and phylogeography of goose parvovirus. Comparative immunology, microbiology and infectious diseases, 102, 102079. https://doi.org/10.1016/j.cimid.2023.102079
Jarvis D. L. (2009). Baculovirus-insect cell expression systems. Methods in enzymology, 463, 191–222. https://doi.org/10.1016/S0076-6879(09)63014-7
Ju, H., Wei, N., Wang, Q., Wang, C., Jing, Z., Guo, L., Liu, D., Gao, M., Ma, B., & Wang, J. (2011). Goose parvovirus structural proteins expressed by recombinant baculoviruses self-assemble into virus-like particles with strong immunogenicity in goose. Biochemical and biophysical research communications, 409(1), 131–136.https://doi.org/10.1016/j.bbrc.2011.04.129
Kang, W., Suzuki, M., Zemskov, E., Okano, K., & Maeda, S. (1999). Characterization of baculovirus repeated open reading frames (bro) in Bombyx mori nucleopolyhedrovirus. Journal of virology, 73(12), 10339–10345. https://doi.org/10.1128/JVI.73.12.10339-10345.1999
Kato, T., Sugioka, S., Itagaki, K., & Park, E. Y. (2016). Gene transduction in mammalian cells using Bombyx mori nucleopolyhedrovirus assisted by glycoprotein 64 of Autographa californica multiple nucleopolyhedrovirus. Scientific Reports, 6(1). https://doi.org/10.1038/srep32283
Kelly, B. J., King, L. A., & Possee, R. D. (2016). Introduction to Baculovirus Molecular Biology. Methods in molecular biology (Clifton, N.J.), 1350, 25–50. https://doi.org/10.1007/978-1-4939-3043-2_2
Kwang, T. W., Zeng, X., & Wang, S. (2016). Manufacturing of AcMNPV baculovirus vectors to enable gene therapy trials. Molecular therapy. Methods & clinical development, 3, 15050. https://doi.org/10.1038/mtm.2015.50
Lee, C., Kim, M., Chun, J., Kim, S., Yoon, D., Lee, H., Bang, H., Lee, H. J., Park, H., & Kim, Y. B. (2024). Baculovirus Vector-Based Varicella-Zoster Virus Vaccine as a Promising Alternative with Enhanced Safety and Therapeutic Functions. Vaccines, 12(3), 333. https://doi.org/10.3390/vaccines12030333
Li, Y. T., Wang, C. L., Zheng, X. X., Wang, H. L., Zhao, Y. K., Gai, W. W., Jin, H. L., Gao, Y. W., Li, N., Yang, S. T., & Xia, X. Z. (2016). Development and characterization of Rift Valley fever virus-like particles. Genetics and molecular research : GMR, 15(1), 10.4238/gmr.15017772. https://doi.org/10.4238/gmr.15017772
Liu, X., Li, Y., Hu, X., Yi, Y., & Zhang, Z. (2017). Gene delivery and gene expression in vertebrate using baculovirus Bombyx mori nucleopolyhedrovirus vector. Oncotarget, 8(62), 106017–106025. https://doi.org/10.18632/oncotarget.22522
Liu, Y., Han, X., Qiao, Y., Wang, T., & Yao, L. (2023). Porcine Deltacoronavirus-like Particles Produced by a Single Recombinant Baculovirus Elicit Virus-Specific Immune Responses in Mice. Viruses, 15(5), 1095. https://doi.org/10.3390/v15051095
Luckow, V. A. (1993). Baculovirus systems for the expression of human gene products, Current Opinion in Biotechnology,4(5), 564-572. https://doi.org/10.1016/0958-1669(93)90078-B.
Maeda, S., Kamita, S. G., Kondo, A. (1993). Host range expansion of Autographa californica nuclear polyhedrosis virus (NPV) following recombination of a 0.6-kilobase-pair DNA fragment originating from Bombyx mori NPV. Journal of Virology, 67, 6234-6238. https://doi.org/10.1128/jvi.67.10.6234-6238.1993
Martínez-Solís, M., Gómez-Sebastián, S., Escribano, J. M., Jakubowska, A. K., & Herrero, S. (2016). A novel baculovirus-derived promoter with high activity in the baculovirus expression system. PeerJ, 4, e2183. https://doi.org/10.7717/peerj.2183
Metz, S. W., & Pijlman, G. P. (2011). Arbovirus vaccines; opportunities for the baculovirus-insect cell expression system. Journal of invertebrate pathology107 Suppl, S16–S30. https://doi.org/10.1016/j.jip.2011.05.002
Miao, Q., Nguyen, W., Zhu, J., Liu, G., van Oers, M. M., Tang, B., Yan, K., Larcher, T., Suhrbier, A., & Pijlman, G. P.(2024). A getah virus-like-particle vaccine providescomplete protection from viremia and arthritis in wild-type mice. Vaccine, 42(25), 126136.https://doi.org/10.1016/j.vaccine.2024.07.037
Miele, S. A., Garavaglia, M. J., Belaich, M. N., & Ghiringhelli, P. D. (2011). Baculovirus: molecular insights on their diversity and conservation. International journal of evolutionary biology, 2011, 379424. https://doi.org/10.4061/2011/379424
Mishra V. (2020). A Comprehensive Guide to the Commercial Baculovirus Expression Vector Systems for Recombinant Protein Production. Protein and peptide letters, 27(6), 529–537. https://doi.org/10.2174/0929866526666191112152646
Murphy, C. I., & Piwnica-Worms, H. (2001). Overview of the baculovirus expression system. Current protocols in neuroscience. https://doi.org/10.1002/0471142301.ns0418s10.
Nguyen, H. T., Falzarano, D., Gerdts, V., & Liu, Q. (2024). Construction and immunogenicity of SARS-CoV-2 virus-like particle expressed by recombinant baculovirus BacMam. Microbiology spectrum, 12(8), e0095924. https://doi.org/10.1128/spectrum.00959-24
OECD (2023). Guidance document on Baculoviruses as plant protection products, Series on Pesticides and Biocides, OECD Publishing, Paris, https://doi.org/10.1787/8f0dc501-en.
Ono, C., Okamoto, T., Abe, T., & Matsuura, Y. (2018). Baculovirus as a Tool for Gene Delivery and Gene Therapy. Viruses, 10(9), 510. https://doi.org/10.3390/v10090510
Pidre, M. L., Arrías, P. N., Amorós Morales, L. C., & Romanowski, V. (2023). The Magic Staff: A Comprehensive Overview of Baculovirus-Based Technologies Applied to Human and Animal Health. Viruses, 15(1), 80. https://doi.org/10.3390/v15010080
Possee, R. D., Griffiths, C. M., Hitchman, R. B., Chambers, A., Murguia-Meca, F., Danquah, J., King, L. A. (2010). Baculoviruses: biology, replication and exploitation. In: Asgari, S and Johnson, K Insect Virology. Great Britain: Caister Academic Press. p35-58.
Puente-Massaguer, E., Lecina, M., & Gòdia, F. (2020). Application of advanced quantification techniques in nanoparticle-based vaccine development with the Sf9 cell baculovirus expression system. Vaccine, 38(7), 1849–1859. https://doi.org/10.1016/j.vaccine.2019.11.087
Razavi-Nikoo, H., Behboudi, E., Aghcheli, B., Hashemi, S. M.A., & Moradi, A. (2023). Bac to Bac SystemEfficiency for Preparing HPV Type 16 Virus-Like ParticleVaccine. Archives of Razi Institute, 78(3), 997–1003.https://doi.org/10.22092/ARI.2023.361975.2708
Rohrmann GF. Baculovirus Molecular Biology [Internet]. 4th edition. Bethesda (MD): National Center for Biotechnology Information (US); 2019. Chapter 9, Baculoviruses as insecticides: Four examples. Available from: https://www.ncbi.nlm.nih.gov/books/NBK543459/
Rychlowska, M., Gromadzka, B., Bieńkowska-Szewczyk, K., & Szewczyk, B. (2011). Application of baculovirus-insect cell expression system for human therapy. Current pharmaceutical biotechnology, 12(11), 1840–1849. https://doi.org/10.2174/138920111798377012
Saika, K., Kato, M., Sanada, H., Matsushita, S., Matsui, M., Handa, H., & Kawano, M. (2020). Induction of adaptive immune responses against antigens incorporated within the capsid of simian virus 40. The Journal of general virology, 101(8), 853–862. https://doi.org/10.1099/jgv.0.001445
Sandro, Q., Benchaouir, R. (2019) Baculovirus: A Powerful Tool for Various Biotechnological Applications. Advances in Biochemistry and Biotechnology, 7, 1085. DOI: 10.29011/2574-7258.001085
Sari, D., Gupta, K., Thimiri Govinda Raj, D. B., Aubert, A., Drncová, P., Garzoni, F., Fitzgerald, D., & Berger, I. (2016). The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics. Advances in experimental medicine and biology, 896, 199–215. https://doi.org/10.1007/978-3-319-27216-0_13
Schaly, S., Ghebretatios, M., & Prakash, S. (2021). Baculoviruses in Gene Therapy and Personalized Medicine. Biologics : targets & therapy, 15, 115–132. https://doi.org/10.2147/BTT.S292692
Skoberne, M., Cardin, R., Lee, A., Kazimirova, A., Zielinski, V., Garvie, D., Lundberg, A., Larson, S., Bravo, F. J., Bernstein, D. I., Flechtner, J. B., & Long, D. (2013). An adjuvanted herpes simplex virus 2 subunit vaccine elicits a T cell response in mice and is an effective therapeutic vaccine in Guinea pigs. Journal of virology, 87(7), 3930–3942. https://doi.org/10.1128/JVI.02745-12
Sokolenko, S., George, S., Wagner, A., Tuladhar, A., Andrich, J. M., & Aucoin, M. G. (2012). Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks. Biotechnology advances, 30(3), 766–781. https://doi.org/10.1016/j.biotechadv.2012.01.009
Tang, Y., Saul, J., Nagaratnam, N. ,Martin-Garcia , J. M., Fromme ,P., Qiu, J., & LaBaer, J. (2020). Construction of gateway-compatible baculovirus expression vectors for high-throughput protein expression and in vivo microcrystal screening. Scientific Report, 10, 13323. https://doi.org/10.1038/s41598-020-70163-2
Thiem, S. M., Cheng, X. W. (2009). Baculovirus host-range. Virologica Sinica, 24 (5), 436–457. https://doi.org/10.1007/s12250-009-3058-8
Tjia, S. T., zu Altenschildesche, G. M., & Doerfler, W. (1983). Autographa californica nuclear polyhedrosis virus (AcNPV) DNA does not persist in mass cultures ofmammalian cells. Virology, 125(1), 107–117. https://doi.org/10.1016/0042-6822(83)90067-3
van Oers M. M. (2011). Opportunities and challenges for the baculovirus expression system. Journal of invertebrate pathology, 107 Suppl, S3–S15. https://doi.org/10.1016/j.jip.2011.05.001
Wang, C. Y., Li, F., Yang, Y., Guo, H. Y., Wu, C. X., & Wang, S.(2006). Recombinant baculovirus containing thediphtheria toxin A gene for malignant glioma therapy.Cancer research, 66(11), 5798–5806.https://doi.org/10.1158/0008-5472.CAN-05-4514
Wang, F., Sun, J., Guo, W., & Wu, Y. (2024). Application of the Insect Cell-Baculovirus Expression Vector System in Adeno-Associated Viral Production. Applied Sciences, 14(23), 10948. https://doi.org/10.3390/app142310948
Wang, Z., Liu, M., Zhao, H., Wang, P., Ma, W., Zhang, Y., Wu, W., & Peng, C. (2021). Induction of Robust and Specific Humoral and Cellular Immune Responses by Bovine Viral Diarrhea Virus Virus-Like Particles (BVDV-VLPs) Engineered with Baculovirus Expression Vector System. Vaccines, 9(4), 350. https://doi.org/10.3390/vaccines9040350
Yang, Y. H., Tai, C. H., Cheng, D., Wang, Y. F., & Wang, J. R. (2022). Investigation of Avian Influenza H5N6 Virus-like Particles as a Broad-Spectrum Vaccine Candidate against H5Nx Viruses. Viruses, 14(5), 925. https://doi.org/10.3390/v14050925
Yin, X., Li, Z., Li, J., Yi, Y., Zhang, Y., Li, X., Li, B., Yang, B., Lan, X., Li, Y., Jiao, W., Zhang, Z., & Liu, J. (2013). Rabies virus nucleoprotein expressed in silkworm pupae at high-levels and evaluation of immune responses in mice. Journal of biotechnology, 163(3), 333–338. https://doi.org/10.1016/j.jbiotec.2012.11.002
Yu, W., Li, J., Wang, M., Quan, Y., Chen, J., Nie, Z., Lv, Z.., & Zhang, Y. (2012). The screening and functional study of proteins binding with the BmNPV polyhedrin promoter. Virology Journal, 6( 90). https://doi.org/10.1186/1743-422X-9-90
Zhang, G., Wang, P., Jiang, L., Wang, S., Zhang, S., & Li, Y. (2023). Evaluation of the immunogenicity of vaccine candidates developed using a baculovirus surface display system for Crimean-Congo hemorrhagic fever virus in mice. Frontiers in microbiology, 14, 1107874. https://doi.org/10.3389/fmicb.2023.1107874
Zhang, Q., Sun, Y., Sun, Y., Zhang, H., & Yang, R. (2024). Expression of VP2 protein of novel goose parvovirus in baculovirus and evaluation of its immune effect. Microbial pathogenesis, 195, 106751. https://doi.org/10.1016/j.micpath.2024.106751

Details

Primary Language

English

Subjects

Veterinary Virology

Journal Section

Review

Authors

Remziye Özbek ^*
0000-0001-9831-7193
Türkiye

Early Pub Date

June 30, 2025

Publication Date

June 30, 2025

Submission Date

June 4, 2025

Acceptance Date

June 24, 2025

Published in Issue

Year 2025 Volume: 7 Number: 1

DOI

https://doi.org/10.51755/turkvetj.1713887

IZ

https://izlik.org/JA94ME67DX

Cite

RIS / Bibtex

APA

Özbek, R. (2025). The Biotechnological Potential of Baculoviruses: From Insect Viruses to Biotechnology Workhorse. Turkish Veterinary Journal, 7(1), 19-30. https://doi.org/10.51755/turkvetj.1713887