Research Article
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Changes in some electrolyte concentrations in lambs with endotoxemia

Year 2023, Volume: 5 Issue: 2, 54 - 57, 31.12.2023
https://doi.org/10.51755/turkvetj.1395131

Abstract

The aim of this study was to reveal the changes in some blood electrolyte levels after LPS infusion in lambs. In the study, 8 Akkaraman breed lambs of both genders, aged 1-3 months, were used. To induce endotoxemia, LPS at a dose of 10 μg/kg was administered intravenously through the jugular vein in 50 ml of 0.9% NaCl over 30 minutes. Clinical examinations were performed and blood samples were taken before LPS administration and at the 2nd, 4th, 6th, 12th and 24th hours after the administration. Sodium (Na+), potassium (K+), chloride (Cl-) and ionized calcium (Ca+2) analyzes of the blood samples were performed on the blood gas device as soon as the blood was taken, without adding any anticoagulant substance. While a significant decrease in K+ level was observed at the 2nd hour after LPS infusion, a significant increase was detected at the 12th hour. While there was no significant change in Na+ and Cl- levels, the Ca+2 level decreased at all hours compared to the beginning, but no statistical difference was determined. As a result, it was evaluated that acute endotoxemia in lambs causes changes in K+ and Ca+2 levels and that monitoring these electrolytes in endotoxemic patients may be useful.

References

  • Agwunobi, A.O., Reid, C., Maycock, P., Little, R.A., & Carlson, G.L. (2000). Insulin resistance and substrate utilization in human endotoxemia. The Journal of Clinical Endocrinology & Metabolism, 85(10), 3770-3778. https://doi.org/10.1210/jcem.85.10.6914.
  • Altınok Yipel, F., & Yipel, M. (2022). Investigation of the concentrations of some essential elements in LPS-induced septicemic sheep. Kocatepe Veterinary Journal, 15(3), 297-302. https://doi.org/10.30607/kvj.1123763.
  • Andersen, H.P. (2003). Bovine endotoxicosis – Some aspects of relevance to production diseases a review. Acta Veterinaria Scandinavica, 98(Suppl),141-155.
  • Aydoğdu, U., Gülersoy, E., & Şen, İ. (2018). Buzağı ishalleri ve oral sıvı takviyeleri. Turkiye Klinikleri Animal Nutrition and Nutritional Diseases -Special Topics, 4(1), 56-64.
  • Basoglu, A., & Aydogdu, U. (2013). Terminal atrial standstill with ventricular escape rhythm in a neonatal calf with acute diarrhea. Turkish Journal of Veterinary & Animal Sciences, 37, 362-365. https://doi.org/10.3906/vet-1202-27.
  • Bundgaard, H., Kjeldsen, K., Suarez Krabbe, K., van Hall, G., Simonsen, L., Qvist, J., Hansen, C.M., Moller, K., Fonsmark, L., Lav Madsen, P., & Klarlund Pedersen, B. (2003). Endotoxemia stimulates skeletal muscle Na+-K+-ATPase and raises blood lactate under aerobic conditions in humans. The American Journal of Physiology-Heart and Circulatory Physiology, 284(3), H1028-1034. https://doi.org/10.1152/ajpheart.00639.2002.
  • Carlstedt, F., Lind, L., Rastad, J., Stjernström, H., Wide, L., & Ljunghall, S. (1998). Parathyroid hormone and ionized calcium levels are related to the severity of illness and survival in critically ill patients. European Journal of Clinical Investigation, 28(11), 898-903. https://doi.org/10.1046/j.1365-2362.1998.00391.x.
  • Clausen, T. (1998). Clinical and therapeutic significance of the Na+,K+ pump*. Clinical Science (London), 95, 3–17.
  • Constable, P.D., Hinchcliff, K.W., Done, S.H., & Grünberg, W.(2017). Veterinary Medicine. A textbook of thediseases of cattle, horses, sheep, pigs, and goats. 11thed., Saunders Ltd, Philadelphia.
  • Coşkun A., & Şen İ. (2012a). Haematological, Biochemical and Coagulation Changes in Calves with Endotoxemia. Agric J, 7(1),37-41.
  • Coskun, A., & Sen, İ. (2012b). Acute phase response and clinical changes in calves with lipopolysaccharide induced endotoxemia. Eurasian Journal of Veterinary Sciences, 28(1), 21-26.
  • Coskun, A., Aydogdu, U., Guzelbektes, H., & Sen, I. (2020a). The levels of trace elements and macrominerals in calves with sepsis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(3), 351-355.https://doi.org/10.9775/kvfd.2019.23187.
  • Coşkun, A., Aydoğdu, U., Başbuğ, O., Turk, S., & Agaoğlu Z.T. (2020b). Determination of the prevalence of electrolyte disorders in newborn calves with diarrhea. Turkish Veterinary Journal, 2(2), 62-66.
  • Fessler, J.F., Bottoms, G.D., Roesel, O.F., Moore, A.B., Frauenfelder, H.C., & Boon, G.D. (1982). Endotoxin-induced change in hemograms, plasma enzymes, and blood chemical values in anesthetized ponies: effects of flunixin meglumine. American Journal of Veterinary Research, 43(1), 140-144.
  • Grinevich, V., Knepper, M.A., Verbalis, J., Reyes, I., & Aguilera, G. (2004). Acute endotoxemia in rats induces down-regulation of V2 vasopressin receptors and aquaporin-2 content in the kidney medulla. Kidney International, 65(1), 54-62. https://doi.org/10.1111/j.1523-1755.2004.00378.x.
  • Lee, J.W. (2010). Fluid and electrolyte disturbances in critically ill patients. Electrolyte Blood Press, 8(2), 72-81.https://doi.org/10.5049/EBP.2010.8.2.72.
  • Lind, L., Carlstedt, F., Rastad, J., Stiernström, H., Stridsberg, M., Ljunggren, O., Wide, L., Larsson, A., Hellman, P., & Ljunghall, S. (2000). Hypocalcemia and parathyroid hormone secretion in critically ill patients. Critical Care Medicine, 28(1), 93-99. https://doi.org/10.1097/00003246-200001000-00015.
  • Naylor D., Sharma A., Li Z., Monteith G., Sullivan T., Canovas A., Mallard B.A., Baes C., Karrow N.A. (2020). Short communication: Characterizing ovine serum stress biomarkers during endotoxemia, Journal of Dairy Science, 103(6), 5501-5508. https://doi.org/10.3168/jds.2019-17718.
  • Pfortmueller, C.A., Uehlinger, D., von Haehling, S., & Schefold, J.C. (2018). Serum chloride levels in critical illness-the hidden story. Intensive Care Medicine Experimental, 6, 10. https://doi.org/10.1186/s40635-018-0174-5.
  • Sayeed, M.M. (1986). Alterations in cellular Ca21 regulation in the liver in endotoxic shock. American Physiological Society, 250, R884–R891. https://doi.org/10.1152/ajpregu.1986.250.5.R884.
  • Smith, G.W. (2009). Treatment of calf diarrhea: oral fluid therapy. Veterinary Clinics of North America: Food Animal Practice, 25(1), 55-72. https://doi.org/10.1016/j.cvfa.2008.10.006.
  • Toribio, R.E., Kohn, C.W., Chew, D.J., Sams, R.A., & Rosol, T.J. (2001). Comparison of serum parathyroid hormone and ionized calcium and magnesium concentrations and fractional urinary clearance of calcium and phosphorus in healthy horses and horses with enterocolitis. American Journal of Veterinary Research, 62(6), 938-947. https://doi.org/10.2460/ajvr.2001.62.938.
  • Toribio, R.E., Kohn, C.W., Hardy, J., & Rosol, T.J. (2005). Alterations in serum parathyroid hormone and electrolyte concentrations and urinary excretion of electrolytes in horses with induced endotoxemia. Journal of Veterinary Internal Medicine, 19(2), 223-231. https://doi.org/10.1892/0891-6640(2005)19<223:aispha>2.0.co;2.
  • Waldron, M.R., Nonnecke, B.J., Nishida, T., Horst, R.L., & Overton, T.R. (2003). Effect of lipopolysaccharide infusion on serum macromineral and vitamin D concentrations in dairy cows. Journal of Dairy Science, 86(11), 3440-3446. https://doi.org/10.3168/jds.S0022-0302(03)73948-4
  • Zaloga, G.P. (2000). Ionized hypocalcemia during sepsis. Critical Care Medicine, 28(1), 266–268. https://doi.org/10.1097/00003246-200001000-00054.

Endotoksemili kuzularda bazı elektrolit konsantrasyolarındaki değişimler

Year 2023, Volume: 5 Issue: 2, 54 - 57, 31.12.2023
https://doi.org/10.51755/turkvetj.1395131

Abstract

Bu çalışmanın amacı, kuzularda LPS infüzyonu sonrası bazı kan elektrolit seviyelerindeki değişimlerin ortaya konulmasıydı. Çalışmada her iki cinsiyetten 1-3 aylık yaşta 8 adet Akkaraman ırkı kuzu kullanıldı. Endotokseminin oluşturulması için 10 μg/kg dozda LPS 50 ml’lik %0.9 NaCl içerinde 30 dakikada vena jugularisten intravenöz olarak uygulandı. LPS uygulamasından önce ve uygulama sonrası 2., 4., 6., 12. ve 24. saatlerde klinik muayeneleri yapılarak kan örnekleri alındı. Alınan kan örneklerinden sodyum (Na+), potasyum (K+), klor (Cl-) ve iyonize kalsiyum (Ca+2) analizleri kan gaz cihazında herhangi bir antikoagülan madde katılmaksızın kan alınır alınmaz yapıldı. LPS infüzyonu sonrası 2. saatte K seviyesinde önemli bir düşüş gözlenirken takibinde 12. saatte önemli bir artış tespit edildi. Na+ ve Cl- seviyesinde önemli herhangi bir değişim göze çarpmazken, Ca+2 seviyesi başlangıca göre tüm saatlerde azalmış ancak istatistiksel bir farklılık belirlenmemiştir. Sonuç olarak, kuzularda akut endotokseminin K+ ve Ca+2 seviyelerinde değişikliğe neden olduğu ve endotoksemik hastalarda bu elektrolitlerin izlenmesinin yararlı olabileceği değerlendirildi.

Supporting Institution

Sivas Cumhuriyet Üniversitesi Bilimsel Araştırma Projeleri Başkanlığı

References

  • Agwunobi, A.O., Reid, C., Maycock, P., Little, R.A., & Carlson, G.L. (2000). Insulin resistance and substrate utilization in human endotoxemia. The Journal of Clinical Endocrinology & Metabolism, 85(10), 3770-3778. https://doi.org/10.1210/jcem.85.10.6914.
  • Altınok Yipel, F., & Yipel, M. (2022). Investigation of the concentrations of some essential elements in LPS-induced septicemic sheep. Kocatepe Veterinary Journal, 15(3), 297-302. https://doi.org/10.30607/kvj.1123763.
  • Andersen, H.P. (2003). Bovine endotoxicosis – Some aspects of relevance to production diseases a review. Acta Veterinaria Scandinavica, 98(Suppl),141-155.
  • Aydoğdu, U., Gülersoy, E., & Şen, İ. (2018). Buzağı ishalleri ve oral sıvı takviyeleri. Turkiye Klinikleri Animal Nutrition and Nutritional Diseases -Special Topics, 4(1), 56-64.
  • Basoglu, A., & Aydogdu, U. (2013). Terminal atrial standstill with ventricular escape rhythm in a neonatal calf with acute diarrhea. Turkish Journal of Veterinary & Animal Sciences, 37, 362-365. https://doi.org/10.3906/vet-1202-27.
  • Bundgaard, H., Kjeldsen, K., Suarez Krabbe, K., van Hall, G., Simonsen, L., Qvist, J., Hansen, C.M., Moller, K., Fonsmark, L., Lav Madsen, P., & Klarlund Pedersen, B. (2003). Endotoxemia stimulates skeletal muscle Na+-K+-ATPase and raises blood lactate under aerobic conditions in humans. The American Journal of Physiology-Heart and Circulatory Physiology, 284(3), H1028-1034. https://doi.org/10.1152/ajpheart.00639.2002.
  • Carlstedt, F., Lind, L., Rastad, J., Stjernström, H., Wide, L., & Ljunghall, S. (1998). Parathyroid hormone and ionized calcium levels are related to the severity of illness and survival in critically ill patients. European Journal of Clinical Investigation, 28(11), 898-903. https://doi.org/10.1046/j.1365-2362.1998.00391.x.
  • Clausen, T. (1998). Clinical and therapeutic significance of the Na+,K+ pump*. Clinical Science (London), 95, 3–17.
  • Constable, P.D., Hinchcliff, K.W., Done, S.H., & Grünberg, W.(2017). Veterinary Medicine. A textbook of thediseases of cattle, horses, sheep, pigs, and goats. 11thed., Saunders Ltd, Philadelphia.
  • Coşkun A., & Şen İ. (2012a). Haematological, Biochemical and Coagulation Changes in Calves with Endotoxemia. Agric J, 7(1),37-41.
  • Coskun, A., & Sen, İ. (2012b). Acute phase response and clinical changes in calves with lipopolysaccharide induced endotoxemia. Eurasian Journal of Veterinary Sciences, 28(1), 21-26.
  • Coskun, A., Aydogdu, U., Guzelbektes, H., & Sen, I. (2020a). The levels of trace elements and macrominerals in calves with sepsis. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 26(3), 351-355.https://doi.org/10.9775/kvfd.2019.23187.
  • Coşkun, A., Aydoğdu, U., Başbuğ, O., Turk, S., & Agaoğlu Z.T. (2020b). Determination of the prevalence of electrolyte disorders in newborn calves with diarrhea. Turkish Veterinary Journal, 2(2), 62-66.
  • Fessler, J.F., Bottoms, G.D., Roesel, O.F., Moore, A.B., Frauenfelder, H.C., & Boon, G.D. (1982). Endotoxin-induced change in hemograms, plasma enzymes, and blood chemical values in anesthetized ponies: effects of flunixin meglumine. American Journal of Veterinary Research, 43(1), 140-144.
  • Grinevich, V., Knepper, M.A., Verbalis, J., Reyes, I., & Aguilera, G. (2004). Acute endotoxemia in rats induces down-regulation of V2 vasopressin receptors and aquaporin-2 content in the kidney medulla. Kidney International, 65(1), 54-62. https://doi.org/10.1111/j.1523-1755.2004.00378.x.
  • Lee, J.W. (2010). Fluid and electrolyte disturbances in critically ill patients. Electrolyte Blood Press, 8(2), 72-81.https://doi.org/10.5049/EBP.2010.8.2.72.
  • Lind, L., Carlstedt, F., Rastad, J., Stiernström, H., Stridsberg, M., Ljunggren, O., Wide, L., Larsson, A., Hellman, P., & Ljunghall, S. (2000). Hypocalcemia and parathyroid hormone secretion in critically ill patients. Critical Care Medicine, 28(1), 93-99. https://doi.org/10.1097/00003246-200001000-00015.
  • Naylor D., Sharma A., Li Z., Monteith G., Sullivan T., Canovas A., Mallard B.A., Baes C., Karrow N.A. (2020). Short communication: Characterizing ovine serum stress biomarkers during endotoxemia, Journal of Dairy Science, 103(6), 5501-5508. https://doi.org/10.3168/jds.2019-17718.
  • Pfortmueller, C.A., Uehlinger, D., von Haehling, S., & Schefold, J.C. (2018). Serum chloride levels in critical illness-the hidden story. Intensive Care Medicine Experimental, 6, 10. https://doi.org/10.1186/s40635-018-0174-5.
  • Sayeed, M.M. (1986). Alterations in cellular Ca21 regulation in the liver in endotoxic shock. American Physiological Society, 250, R884–R891. https://doi.org/10.1152/ajpregu.1986.250.5.R884.
  • Smith, G.W. (2009). Treatment of calf diarrhea: oral fluid therapy. Veterinary Clinics of North America: Food Animal Practice, 25(1), 55-72. https://doi.org/10.1016/j.cvfa.2008.10.006.
  • Toribio, R.E., Kohn, C.W., Chew, D.J., Sams, R.A., & Rosol, T.J. (2001). Comparison of serum parathyroid hormone and ionized calcium and magnesium concentrations and fractional urinary clearance of calcium and phosphorus in healthy horses and horses with enterocolitis. American Journal of Veterinary Research, 62(6), 938-947. https://doi.org/10.2460/ajvr.2001.62.938.
  • Toribio, R.E., Kohn, C.W., Hardy, J., & Rosol, T.J. (2005). Alterations in serum parathyroid hormone and electrolyte concentrations and urinary excretion of electrolytes in horses with induced endotoxemia. Journal of Veterinary Internal Medicine, 19(2), 223-231. https://doi.org/10.1892/0891-6640(2005)19<223:aispha>2.0.co;2.
  • Waldron, M.R., Nonnecke, B.J., Nishida, T., Horst, R.L., & Overton, T.R. (2003). Effect of lipopolysaccharide infusion on serum macromineral and vitamin D concentrations in dairy cows. Journal of Dairy Science, 86(11), 3440-3446. https://doi.org/10.3168/jds.S0022-0302(03)73948-4
  • Zaloga, G.P. (2000). Ionized hypocalcemia during sepsis. Critical Care Medicine, 28(1), 266–268. https://doi.org/10.1097/00003246-200001000-00054.

Details

Primary Language Turkish
Subjects Veterinary Sciences (Other)
Journal Section Research Article
Authors

Alparslan COŞKUN 0000-0002-2242-9647

Uğur AYDOĞDU 0000-0002-9828-9863

Emre ARSLANBAŞ 0000-0003-0030-7195

Project Number V-048
Early Pub Date December 27, 2023
Publication Date December 31, 2023
Submission Date November 24, 2023
Acceptance Date December 19, 2023
Published in Issue Year 2023Volume: 5 Issue: 2

Cite

APA COŞKUN, A., AYDOĞDU, U., & ARSLANBAŞ, E. (2023). Endotoksemili kuzularda bazı elektrolit konsantrasyolarındaki değişimler. Turkish Veterinary Journal, 5(2), 54-57. https://doi.org/10.51755/turkvetj.1395131