Anticancerogenic Effects of Silymarin on Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats

Emin Karakurt; Serpil Dağ

EN TR

Anticancerogenic Effects of Silymarin on Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats

Abstract

The aim of this study was to evaluate the anticancerogenic effect of Silimarin by immunohistochemical methods on Diethylnitrosamine (DEN) induced Hepatocellular carcinoma (HCC) model in Wistar rats. Fifty male Wistar-Albino rats aging 2 months were randomly divided into 5 equal groups. The Control group rats were given pellet feed and drinking water for 20 weeks. The Sham group rats were injected intraperitoneally Propylene glycol dissolved in %0.9 NaCl three times a week. The Silymarin group rats were injected intraperitoneally 100 mg/kg Silymarin three times a week for 20 weeks. The DEN group rats were injected intraperitoneally 50 mg/kg DEN once a week for 20 weeks. The DEN+Silymarin group rats were injected 50 mg/kg DEN once a week for 20 weeks. Silymarin at a dose of 100 mg/kg was injected intraperitoneally for 21 weeks, three times a week, starting 1 week before DEN administration. At the end of 21 weeks, macroscopically multifocal nodular structures were detected in the DEN and DEN+Silymarin group. A mix pattern of trabecular and acinar structures of HCC was observed in DEN and DEN+Silymarin groups. Besides; No statistically significant results were found between DEN and DEN + Silymarin groups in terms of iNOS, Nitrotyrosine, PCNA, GSTPi, MMP-9, HPIP and TUNEL staining (P > 0.05).

Keywords

Diethylnitrosamine,hepatocellular carcinoma,liver cancer,rats,silymarin

Wistar Ratlarında Dietilnitrozamin Uygulanarak Oluşturulan Hepatoselüler Karsinom Modelinde Silimarinin Antikanserojenik Etkileri

Öz

Bu çalışmada Wistar ratlarında Dietilnitrozamin (DEN) uygulaması yapılarak oluşturulan hepatoselüler karsinom (HCC) modelinde, Silimarin’in antikanserojenik etkileri immunohistokimyasal yöntemlerle araştırılması amaçlandı. 50 adet 2 aylık Wistar Albino cinsi erkek rat rastgele olacak şekilde 5 gruba ayrıldı. Kontrol grubundaki ratlara 20 hafta boyunca pelet yem ve içme suyu verildi. Sham grubundaki hayvanlara % 0.9 NaCl içerisinde çözdürülmüş propilen glikol intraperitoneal yolla haftada 3 kez olmak üzere 20 hafta boyunca verildi. DEN grubundaki hayvanlara 50 mg/kg dozunda haftada 1 kez olmak üzere 20 hafta boyunca DEN uygulaması yapıldı. DEN+Silimarin grubundaki ratlara DEN uygulamasından 1 hafta önce başlayacak şekilde 100 mg/kg dozunda haftada 3 kez olmak üzere 21 hafta boyunca intraperitoneal yolla Silimarin uygulandı. Silimarin grubundaki ratlara 100 mg/kg dozunda 20 hafta boyunca intraperitoneal yolla haftada 3 kez olmak üzere Silimarin uygulandı. 21 hafta sonunda DEN ve DEN+Silimarin gruplarında makroskobik olarak multifokal nodüler yapılar tespit edildi. Mikroskobik incelemeler sonucunda DEN ve DEN+Silimarin gruplarında HCC’nin trabeküler ve asiner paternini içeren miks tipi tespit edildi. iNOS, Nitrotirozin, PCNA, GSTPi, MMP-9, HPIP ve TUNEL boyamaları sonucunda DEN ve DEN+Silimarin grupları arasında istatistiki karşılaştırmada anlamlı sonuç bulunamadı (P > 0.05).

Anahtar Kelimeler

Dietilnitrozamin,hepatoselüler karsinom,karaciğer kanseri,ratlar,silimarin

Supporting Institution

KAÜ Bilimsel Araştırma Projeleri Koordinatörlüğü

Project Number

2016-TS-08

References

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Details

Primary Language

English

Subjects

Veterinary Surgery

Journal Section

Research Article

Authors

Emin Karakurt ^*
Türkiye

Serpil Dağ
0000-0001-7667-689X
Türkiye

Publication Date

July 13, 2020

Submission Date

December 25, 2019

Acceptance Date

May 11, 2020

Published in Issue

Year 2020 Volume: 2 Number: 1

IZ

https://izlik.org/JA85CE38TJ

Cite

RIS / Bibtex

APA

Karakurt, E., & Dağ, S. (2020). Anticancerogenic Effects of Silymarin on Diethylnitrosamine-Induced Hepatocellular Carcinoma in Wistar Rats. Turkish Veterinary Journal, 2(1), 7-16. https://izlik.org/JA85CE38TJ