Research Article
Mycobacterium bovis ile Enfekte Sığırlarda Akut Faz Protein ve Bazı Biyokimyasal Parametre Düzeyleri
Abstract
Bu çalışmanın amacı Mycobacterium bovis ile enfekte sığırlarda akut faz proteinler (AFP) ve bazı biyokimyasal parametrelerin düzeyinin belirlenmesidir. Çalışmada biyokimyasal parametreleri araştırmak için ELISA test sonuçlarına göre 25 adet M.bovis ile enfekte ve 25 adet sağlıklı sığır kullanıldı. Hayvanların V. jugularis’inden kan örnekleri antiko-agulansız tüplere alındı. Haptoglobin, serum amiloid A (SAA), seruloplazmin, aspartat amino transferaz (AST), alkalen fosfataz (ALP), gama-glutamil transferaz (GGT), üre, kreatinin ve demir (Fe) düzeyleri kolorimetrik olarak tayin edildi. Yapılan analizler sonucunda M.bovis ile enfekte sığırlar ile kontrol grubundaki hayvanlar karşılaştırıldığında AFP’lerden haptoglobin, SAA ve seruloplazmin; biyokimyasal parametrelerden ise AST, ALP, GGT, üre ve kreatinin düzeylerinin kontrol grubuna göre istatistiksel olarak anlamlı derecede yükseldiği, serum Fe düzeyinin ise düştüğü belirlendi. Sonuç olarak M.bovis ile enfekte sığırlarda akut faz yanıt oluştuğu ve bunun sonucu olarak da AFP’lerin arttığı, karaciğer fonksiyonlarının bozulduğu belirlenmiştir.
References
- 1. Alsemgeest SPM, Kalsbeek HC, Wensing T, Koeman JP, van Ederen AM, Gruys E. Concentration of serum amyloid-A (SAA) and haptoglobin (Hp) as parameters of inflammatory diseases in cattle. Vet Q 1994; 16 (1): 21-3. 2. Ayele WY, Neill SD, Zinsstag J, Weiss MG, Pavlik I. Bovine tuberculosis: An old disease but a new threat to Africa. Int J Tuberc Lung Dis 2004; 8(8): 924-37. 3. Aytekin İ, Kalkan Y, Mamak N, Özkan A. Granulomatous lesions in a cow infected with Mycobacterium bovis. J Vet Sci Ataturk University 2009; 4(2): 117-22. 4. Colombo JP, Richterich R. Zur bestimmung des caeruloplasmin im plasma (on the determination of ceruloplasmin in plasma). Schweiz Med Wochenschr 1964; 94: 715- 20. 5. Eckersall PD, Bell R. Acute phase proteins: biomarkers of infection and inflammation in veterinary medicine. Vet J 2010; 185(1): 23- 7. 6. Eckersall PD, Conner JG. Bovine and canine acute phase proteins. Vet Res Commun 1988; 12(2-3): 169-78. 7. Eckersall PD. Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Revue Med Vet 2000; 151(7): 577-84. 8. Ganheim C, Hulten C, Carlsson U, Kindahl H, Niskanen R, Waller KP. The acute phase response in calves experimentally infected with bovine viral diarrhea virus and /or Manheimia haemolytica. J Vet Med Ser B 2003; 50(4): 183-90. 9. Gokce HI, Woldehiwet Z. The effects of Ehrlichia (Cytoecetes) phagochytophila on the clinical chemistry of sheep and goats. J Vet Med 1999; 46(2): 93-103. 10. Grange JM, Kardjito T, Beck JS, Ebeid O, Köhler W, Prokop O. Haptoglobin: An immunoregulatory role in tuberculosis? Tubercle 1985; 66: 41-7. 11. Grange JM, Kardjito T, Setiabudi I. A study of acute-phase reactant proteins in Indonesian patients with pulmonary tuberculosis. Tubercle 1984; 65: 23-39. 12. Gruys E, Obwolo MJ, Toussaint MJM. Diagnostic significance of the major acute phase proteins in veterinary clinical chemistry. A review. Vet Bull 1994; 64(11): 1009-18. 13. Gruys E, Toussaint MJM, Niewold TA, Koopmans SJ. Acute phase reaction and acute phase proteins. J Zhejiang Univ Sci B 2005; 6(11): 1045-56. 14. Hellman NE, Gitlin JD. Ceruloplasmin metabolism and function. Annu Rev Nutr 2002; 22: 439-58. 15. Horadagoda A, Eckersall PD, Hodgson JC, Gibbs HA, Moon GM. Immediate responses in serum TNF alpha and acute phase protein concentrations to infection with Pasteurella haemolytica A1 in calves. Res Vet Sci 1994; 57: 129-32. 16. Horadagoda NU, Knox KMG, Gibbs HA, Reid SWJ, Horadagoda A, Edwards SER, Eckersall PD. Acute phase proteins in cattle discrimination between acute and chronic inflammation. Vet Rec 1999; 144: 437-41. 17. Höfner MC, Fosbery MW, Eckersall PD, Donaldson AL. Haptoglobin response of cattle infected with foot-mouth disease virus. Res Vet Sci 1994; 57(1): 125-8. 18. Javed MT, Usman M, Irfan M, Cagiola M. A study on tuberculosis in buffaloes: some epidemiological aspects, along with haematological and serum protein changes. Veterinarski Arhiv 2006; 76(3): 193-206. 19. Kaneko JJ, Harvey JW, Bruss ML. Clinical Biochemistry of Domestic Animals. Sixth Edition. New York: Academic Press, 2008; p. 364-90. 20. Lopez-Olvera JR, Fernandez-de-Mera IG, Serrano E, Vidal D, Vicente J, Fierro Y, Gortazar C. Sex-related differences in body condition and serum biochemical parameters in red deer (Cervus elaphus) naturally infected with Mycobacterium bovis. Vet J 2013; 198: 702-6. 21. Madebo T, Lindtjorn B, Aukrust P, Berge RK. Circulating antioxidants and lipid peroxidation products in untreated tuberculosis patients in Ethiopia. Am J Clin Nutr 2003; 78: 117-22. 22. Miller LD, Thoen CO, Throlson KJ, Himes EM, Morgan RL. Serum biochemical and hematologic values of normal and Mycobacterium bovis-infected American bison. J VetDiagn Invest 1989; 1: 219-22. 23. Moda G, Daborn CJ, Grange JM, Cosivi O. The zoonotic importance of Mycobacterium bovis. Tuber Lung Dis 1996; 77: 103-8. 24. Murata H, Shimada N, Yoshioka M. Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J 2004; 168(1): 28-40. 25. Neill SD, Pollock, JM, Bryson DB, Hanna J. Pathogenesis of Mycobacterium bovis infection in cattle. Vet Microbiol 1994; 40: 41-52. 26. O'Reilly LM, Daborn CJ. The epidemiology of Mycobacterium bovis infections in animals and man: a review. Tuber Lung Dis 1995; 76: 1-46. 27. Petersen HH, Nielsen JP, Heegaard PM. Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res 2004; 35(2): 163-87. 28. Pollock JM, Neill SD. Mycobacterium bovis infection and tuberculosis in cattle. Vet J 2002; 163: 115-27. 29. Rohini K, Srikumar PS, Jyoti Saxena, Mahesh Kumar A, Surekha Bhat. Assessment of serum calcium, phosphorus, C-reactive protein and procalcitonin in tuberculosis patients. Int J Collab Res Internal Med Public Health 2012; 4(12): 1868-75. 30. Russell KE, Roussel AJ. Evaluation of the ruminant serum chemistry profile. Vet Clin Food Anim 2007; 23(3): 403-26. 31. Sahin F, Yildiz P. Distinctive biochemical changes in pulmonary tuberculosis and pneumonia. Arch Med Sci 2013; 9(4): 656- 61. 32. Schiller I, Oesch B, Vordermeier HM, Palmer MV, Harris BN, Orloski KA, Buddle BM, Thacker TC, Lyashchenko KP, Waters WR. Bovine tuberculosis: A review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication. Transbound Emerg Dis 2010; 57: 205-20. 33. Shettar M, Nalini TS, Anjan Kumar KR, Ravikumar P, Azeemulla HR. Hematological and biochemical studies in tuberculin test positive reactors. Int J Pharma Bio Sci 2011; 2(4): 16-22. 34. Skinner JG, Brown RA, Roberts L. Bovine haptoglobin response in clinically defined field conditions.Vet Rec 1991; 128(7): 147- 9. 35. SPSS. IBM SPSS Statistics for Windows, Version 20.0. New York: Armonk, 2011. 36. Tothova C, Nagy O, Kovac G. Acute phase proteins and their use in the diagnosis of diseases in ruminants: A review. Vet Med 2014; 59(4): 163-80. 37. Toussaint MJM, van Ederen AM, Gruys E. Implication of clinical pathology in assessment of animal health and in animal production and meat inspection. Comp Haematol Int 1995; 5(3): 149-57. 38. Wells B, Innocent GT, Eckersall PD, McCulloch E, Nisbet AJ, Burgess STG. Two major ruminant acute phase proteins, haptoglobin and serum amyloid A, as serum biomarkers during active sheep scab infestation. Vet Res 2013; 44: 103-14.
Levels of Acute Phase Protein and Some Biochemical Parameter in Cattle Infected with Mycobacterium Bovis
Abstract
In this study, it was aimed to determine the levels of acute phase proteins (APP) and some biochemical parameters in cattle infected with Mycobacterium bovis. Twenty-five M. bovis infected and twenty-five antibody-negative healthy bovine sera were used according to ELISA test results to investigate the biochemical parameters. Blood samples obtained from Jugular veins of animals were collected into plain tubes. Haptoglobin, serum amyloid A (SAA), ceruloplasmin, aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), urea, creatinine and iron (Fe) levels were measured colorimetrically. Compared with the control group, cattle with tuberculosis were found have statistically significant increased levels of APP such as serum haptoglobin, SAA and ceruloplasmin and levels of biochemical parameters such as AST, ALP, GGT, urea and creatinine while decreased levels of serum Fe. It was determined that acute phase response occurred in cattle infected with M. bovis which led to increase APP and impairment in the normal functions of the liver.
References
- 1. Alsemgeest SPM, Kalsbeek HC, Wensing T, Koeman JP, van Ederen AM, Gruys E. Concentration of serum amyloid-A (SAA) and haptoglobin (Hp) as parameters of inflammatory diseases in cattle. Vet Q 1994; 16 (1): 21-3. 2. Ayele WY, Neill SD, Zinsstag J, Weiss MG, Pavlik I. Bovine tuberculosis: An old disease but a new threat to Africa. Int J Tuberc Lung Dis 2004; 8(8): 924-37. 3. Aytekin İ, Kalkan Y, Mamak N, Özkan A. Granulomatous lesions in a cow infected with Mycobacterium bovis. J Vet Sci Ataturk University 2009; 4(2): 117-22. 4. Colombo JP, Richterich R. Zur bestimmung des caeruloplasmin im plasma (on the determination of ceruloplasmin in plasma). Schweiz Med Wochenschr 1964; 94: 715- 20. 5. Eckersall PD, Bell R. Acute phase proteins: biomarkers of infection and inflammation in veterinary medicine. Vet J 2010; 185(1): 23- 7. 6. Eckersall PD, Conner JG. Bovine and canine acute phase proteins. Vet Res Commun 1988; 12(2-3): 169-78. 7. Eckersall PD. Recent advances and future prospects for the use of acute phase proteins as markers of disease in animals. Revue Med Vet 2000; 151(7): 577-84. 8. Ganheim C, Hulten C, Carlsson U, Kindahl H, Niskanen R, Waller KP. The acute phase response in calves experimentally infected with bovine viral diarrhea virus and /or Manheimia haemolytica. J Vet Med Ser B 2003; 50(4): 183-90. 9. Gokce HI, Woldehiwet Z. The effects of Ehrlichia (Cytoecetes) phagochytophila on the clinical chemistry of sheep and goats. J Vet Med 1999; 46(2): 93-103. 10. Grange JM, Kardjito T, Beck JS, Ebeid O, Köhler W, Prokop O. Haptoglobin: An immunoregulatory role in tuberculosis? Tubercle 1985; 66: 41-7. 11. Grange JM, Kardjito T, Setiabudi I. A study of acute-phase reactant proteins in Indonesian patients with pulmonary tuberculosis. Tubercle 1984; 65: 23-39. 12. Gruys E, Obwolo MJ, Toussaint MJM. Diagnostic significance of the major acute phase proteins in veterinary clinical chemistry. A review. Vet Bull 1994; 64(11): 1009-18. 13. Gruys E, Toussaint MJM, Niewold TA, Koopmans SJ. Acute phase reaction and acute phase proteins. J Zhejiang Univ Sci B 2005; 6(11): 1045-56. 14. Hellman NE, Gitlin JD. Ceruloplasmin metabolism and function. Annu Rev Nutr 2002; 22: 439-58. 15. Horadagoda A, Eckersall PD, Hodgson JC, Gibbs HA, Moon GM. Immediate responses in serum TNF alpha and acute phase protein concentrations to infection with Pasteurella haemolytica A1 in calves. Res Vet Sci 1994; 57: 129-32. 16. Horadagoda NU, Knox KMG, Gibbs HA, Reid SWJ, Horadagoda A, Edwards SER, Eckersall PD. Acute phase proteins in cattle discrimination between acute and chronic inflammation. Vet Rec 1999; 144: 437-41. 17. Höfner MC, Fosbery MW, Eckersall PD, Donaldson AL. Haptoglobin response of cattle infected with foot-mouth disease virus. Res Vet Sci 1994; 57(1): 125-8. 18. Javed MT, Usman M, Irfan M, Cagiola M. A study on tuberculosis in buffaloes: some epidemiological aspects, along with haematological and serum protein changes. Veterinarski Arhiv 2006; 76(3): 193-206. 19. Kaneko JJ, Harvey JW, Bruss ML. Clinical Biochemistry of Domestic Animals. Sixth Edition. New York: Academic Press, 2008; p. 364-90. 20. Lopez-Olvera JR, Fernandez-de-Mera IG, Serrano E, Vidal D, Vicente J, Fierro Y, Gortazar C. Sex-related differences in body condition and serum biochemical parameters in red deer (Cervus elaphus) naturally infected with Mycobacterium bovis. Vet J 2013; 198: 702-6. 21. Madebo T, Lindtjorn B, Aukrust P, Berge RK. Circulating antioxidants and lipid peroxidation products in untreated tuberculosis patients in Ethiopia. Am J Clin Nutr 2003; 78: 117-22. 22. Miller LD, Thoen CO, Throlson KJ, Himes EM, Morgan RL. Serum biochemical and hematologic values of normal and Mycobacterium bovis-infected American bison. J VetDiagn Invest 1989; 1: 219-22. 23. Moda G, Daborn CJ, Grange JM, Cosivi O. The zoonotic importance of Mycobacterium bovis. Tuber Lung Dis 1996; 77: 103-8. 24. Murata H, Shimada N, Yoshioka M. Current research on acute phase proteins in veterinary diagnosis: an overview. Vet J 2004; 168(1): 28-40. 25. Neill SD, Pollock, JM, Bryson DB, Hanna J. Pathogenesis of Mycobacterium bovis infection in cattle. Vet Microbiol 1994; 40: 41-52. 26. O'Reilly LM, Daborn CJ. The epidemiology of Mycobacterium bovis infections in animals and man: a review. Tuber Lung Dis 1995; 76: 1-46. 27. Petersen HH, Nielsen JP, Heegaard PM. Application of acute phase protein measurements in veterinary clinical chemistry. Vet Res 2004; 35(2): 163-87. 28. Pollock JM, Neill SD. Mycobacterium bovis infection and tuberculosis in cattle. Vet J 2002; 163: 115-27. 29. Rohini K, Srikumar PS, Jyoti Saxena, Mahesh Kumar A, Surekha Bhat. Assessment of serum calcium, phosphorus, C-reactive protein and procalcitonin in tuberculosis patients. Int J Collab Res Internal Med Public Health 2012; 4(12): 1868-75. 30. Russell KE, Roussel AJ. Evaluation of the ruminant serum chemistry profile. Vet Clin Food Anim 2007; 23(3): 403-26. 31. Sahin F, Yildiz P. Distinctive biochemical changes in pulmonary tuberculosis and pneumonia. Arch Med Sci 2013; 9(4): 656- 61. 32. Schiller I, Oesch B, Vordermeier HM, Palmer MV, Harris BN, Orloski KA, Buddle BM, Thacker TC, Lyashchenko KP, Waters WR. Bovine tuberculosis: A review of current and emerging diagnostic techniques in view of their relevance for disease control and eradication. Transbound Emerg Dis 2010; 57: 205-20. 33. Shettar M, Nalini TS, Anjan Kumar KR, Ravikumar P, Azeemulla HR. Hematological and biochemical studies in tuberculin test positive reactors. Int J Pharma Bio Sci 2011; 2(4): 16-22. 34. Skinner JG, Brown RA, Roberts L. Bovine haptoglobin response in clinically defined field conditions.Vet Rec 1991; 128(7): 147- 9. 35. SPSS. IBM SPSS Statistics for Windows, Version 20.0. New York: Armonk, 2011. 36. Tothova C, Nagy O, Kovac G. Acute phase proteins and their use in the diagnosis of diseases in ruminants: A review. Vet Med 2014; 59(4): 163-80. 37. Toussaint MJM, van Ederen AM, Gruys E. Implication of clinical pathology in assessment of animal health and in animal production and meat inspection. Comp Haematol Int 1995; 5(3): 149-57. 38. Wells B, Innocent GT, Eckersall PD, McCulloch E, Nisbet AJ, Burgess STG. Two major ruminant acute phase proteins, haptoglobin and serum amyloid A, as serum biomarkers during active sheep scab infestation. Vet Res 2013; 44: 103-14.
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Details
| Journal Section | Articles |
|---|---|
| Authors | |
| Publication Date | August 15, 2017 |
| Submission Date | September 21, 2017 |
| Acceptance Date | October 25, 2016 |
| Published in Issue | Year 2017 Volume: 14 Issue: 2 |
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https://dergipark.org.tr/tr/download/journal-file/20610
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