Gastrit ve Mide Kanseri Hastalarında Kan Malondialdehit (MDA) ve Redükte Glutatyon(GSH) Düzeylerinin Araştırılması

Aysel Güven; Samet Kısaçam

Research Article

Year 2020, Volume: 7 Issue: 1, 1 - 8, 28.06.2020

Aysel Güven Samet Kısaçam

Abstract

References

1. Jesberger, JA., Richardson JS. (1991). Oxygen free radicals and brain dysfunction.Intern J Neuroscience; 57:1-17
2. Yerer, M.B., Aydoğan, S. (2000). Oksidatif Stres ve Antioksidantlar, Erciyes Univ.Sağlık Bilimleri Dergisi, 9(1): 49-53.
3. Akkus, İ. (1995). Serbest Radikaller ve Fizyopatolojik Etkileri, 1.Baskı, Mimoza Yayınları, Konya.
4. Tekkes, Y. (2006). Streptozotosin ile Diabet Olusturulmus Farelerde Aspirin ve E Vitaminin Dokularda Lipid Peroksidasyonu ve Antioksidan Sisteme Etkisinin Arastırılması, Yuksek Lisans Tezi, Kahramanmaras Sutcu İmam Univ., Fen Bil.Enstitusu, Kahramanmaras.
5. Holley, AE., Cheeseman, KH. (1993). Measuring free radical reactions in vivo. Br.medbull. Jul; 49(3):494-505.
6. Cheeseman, K.H., Slater, T.F. (1993). An Introduction to Free Radical Biochemistry, British Med. Bull., Jul, 49(3): 481-493.
7. Pelayo Correa. (2007). Jeanmarie Houghton, Carcinogenesis of Helicobacter Pylori, Gastroenterology. 133:659–672.
8. Meister, A., Anderson, M.E. (1983). Glutathione, Ann. Rev. Biochem., 52: 711-760.
9. Güven A, Kaya N. (2005). Determination of Reducte Glutathione(GSH), Glutathione S-Transferase(GST) and Selenium(Se) Levels in Goose Liver Cells with Damage Induced by Carbon Tetrachloride (CCl4) and Ethanol ( C2H5OH). Turk. J Vet Anim Sci, 29: 1233-1238.
10. Wallace, JL., Tıgley, AW. (1995). Review article: new insights into prostaglandins and rnucosal defence. Aliment Pharmacol Ther; 9: 227-235.
11. Annie On On Chan, et al. (2014). Risk factors for gastric cancer. UpToDate 17 April .12. Sadler, T.W. (1996). Langman’s Medikal Embriyoloji, Ceviri editoru Prof.Dr. A.Can Basaklar, Palme yayıncılık, 7.baskı, 236–240.
13. Correa, P., Fox, J., at al, Helicobacter Pylori and Gastric Carcinoma: Serum Antiprevalance in Populations with Contrasting Cancer Risks. Cancer, 66:2569, 1990.
14. Gore R. (1997). Gastrointestinal cancer. Radiol Clin North Am, 35:295-310.
15. McLean MH, El-Omar EM. (2014). Genetic of gastric cancer. Nat Rev Gastroenterol Hepatol [Epub ahead of print] Review. PMID: 25134511.
16. Cevikbas, Uğur(Cevirici Editoru), Gastrik Karsinom: Basic Pathology(Temel Patoloji), Altıncı Edisyon, W.B. Saundres Company, Nobel Tıp Kitapevleri, Yuce Reklam/Yayın/Dağıtım a.s., Philadelphia, Đstanbul, 488-489.
17. Ohkawa H., Ohishi N., at al,. (2000). Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction” Anal. Biochem., 95: 351-358 (1979).
18. Sedlak J., Lindsay R.H., Estimation of Total Protein-bound and Non-protein Sulfhdryl Groups in Tissue with Ellman’s Reagent, Anal. Biochem., 25: 192-205 (1968).
19. Hristozov, D., Gadjeva, V., at al. (2001). Evaluation of oxidative stress in patients with cancer. Arch. Physiol. Biochem. 109: 331-336.
20. Draper, HH., Hadley, M. (1990). A review of recent studies on the metabolism of exogenous and endogenous malondialdehyde. Xenobiotica’’ 20: 901-7.
21. Romero, FJ., Morell, B., et al. (1998). Lipid peroxidation products and antioxidants in human disease.’’ Environmental Health Perspectives 106: 1229-34.
22. Klarod K, Hongsprabhas P, Khampitak T, Wirasorn K, Kiertiburanakul S, Tangrassameeprasert R, et al. (2015). Serum antioxidant levels and nutritional status in early and advanced stage lung cancer patients. Nutrition. 27:1156-60.
23. Khanzode, SS., Khanzode, SD., at al. (2003). Serum and plasma concentration of oxidant and antioxidants in patients of Helicobacter pylori gastritis and its correlation with gastric cancer. Cancer Lett. 30; 195: 27-31.
24. Ilhan, N., Ilhan, N., at al. (2004). Reactive protein, procalcitonin, interleukin-6, vascular endothelial growth factor and oxidative metabolites in diagnosis of infection and staging in patients with gastric cancer. World J Gastroenterol. 10: 1115-20.
25. Gerber, M., Astre, C., at al. (1998). Tumour progression and oxidant-antioxidant status. Cancer letters. 14: 211-214.
26. Torun, M., Yardım, S., at al, (1995). Serum beta-carotene, vitamin E, vitamin C and malondialdehyde levels in several types Phar.Ther. 20: 259-263. 20.
27. Saez, GT., Bannister, WH., Bannister JV. (1990). Free radicals and thiol compounds-The role of glutathione against free radical toxicity. In: Vina J (ed). Glutathione Metabo Physiological Functions. Boca Raton, FL: CRC Press. Inc, 237-254.
28. Coles, B., Ketterer, B. (1990). The role of glutathione transferases Rev. Biochem. Mol.Biol. 25: 47-70.
29. Daduk, Y. (2006). Mide Kanserinde Talsialik Asit, Glutatyon, Malondialdehit ve Bu Parametrelerin Birbiriyle ve Kanser Evresi ile İliskisinin İncelenmesi Uzmanlık Tezi İstanbul.
30. Choi, MA., Kim, BS., at al. (1999). Serum antioxidative vitamin levels and lipid peroxidation ingastric carcinoma patients. Cancer Letters 136: 89-93.
31. Baskol, M., Baskol, G., vd, 2007. ‘‘Mide Kanserli Hastalarda Oksidan ve Antioksidan Parametreler ve Birbiriyle Đliskileri’’ Turk Klinik Biyokimya Derg. 5(3):83-89.
32. Asaka M, Sugiyama T, Nobuta A et al. (2001). Atrophic gastritis and Intestinal Metaplasia in Japan: Results of a Large Multicenter Study. Helicobacter. 6:294–299.
33. Cerutti PA. (1985). Pro-oxidant states and tumor promotion. Science. 227(4685):375-81.
34. Shamberger RJ. (1972). Increase of peroxidation in carcinogenesis. J Natl Cancer Inst. 48(5):1491-7.

Gastrit ve Mide Kanseri Hastalarında Kan Malondialdehit (MDA) ve Redükte Glutatyon(GSH) Düzeylerinin Araştırılması

Year 2020, Volume: 7 Issue: 1, 1 - 8, 28.06.2020

Aysel Güven Samet Kısaçam

Abstract

Amaç:
Kanser ve gastrit üzerine antioksidan sistemlerin rolü halen tam olarak
bilinmediği için hastalık süresince artan MDA’in ve düşen GSH'un bu
hastalıklarda rol alıp almadığını belirlemek.

Gereç
ve Yöntem: Araştırma materyalimizde Kafkas Üniversitesi
Araştırma ve Uygulama Merkezi'ne başvuran 13 gastrit hastası, 12 kanser deney
grubunu, sağlıklı 13 birey de kontrol
grubu oluşturdu.

Bulgular:
Yapılan çalışmalarda sağlıklı grupta GSH değeri 1,65 0,43 mol/ml, gastrit
hastası olan grupta 1,89 0,24 mol/ml, mide kanserli hasta grubunda ise 1,51
0,28 mol/ml olarak saptanmıştır. Yine kontrol grubunda MDA değeri 0,67 0,66
nmol/ml, gastrit hastası olan grupta 2,97 1,77 nmol/ml, mide kanseri hastası
olan grupta ise 3,65 2,46 nmol/ml olarak saptanmıştır. Gastrit hastası ve mide
kanseri hastalarında MDA düzeyleri kontrol grubuna göre istatistiksel olarak
anlamlı bir artış (p< 0,05) göstermiştir.

Sonuç: İnsanlarda
mide kanseri etyolojisi halen tam olarak bilinmemekle birlikte, çalışmamızda
azalan antioksidan savunma sistemi, artan oksidatif stres ve lipid
peroksidasyonu gastrik karsinogeneziste bu faktörlerin önemli bir rol oynadığı
söylenebilir.

Keywords

Malondialdehit(MDA), Redükte Glutatyon(GSH)

References

1. Jesberger, JA., Richardson JS. (1991). Oxygen free radicals and brain dysfunction.Intern J Neuroscience; 57:1-17
2. Yerer, M.B., Aydoğan, S. (2000). Oksidatif Stres ve Antioksidantlar, Erciyes Univ.Sağlık Bilimleri Dergisi, 9(1): 49-53.
3. Akkus, İ. (1995). Serbest Radikaller ve Fizyopatolojik Etkileri, 1.Baskı, Mimoza Yayınları, Konya.
4. Tekkes, Y. (2006). Streptozotosin ile Diabet Olusturulmus Farelerde Aspirin ve E Vitaminin Dokularda Lipid Peroksidasyonu ve Antioksidan Sisteme Etkisinin Arastırılması, Yuksek Lisans Tezi, Kahramanmaras Sutcu İmam Univ., Fen Bil.Enstitusu, Kahramanmaras.
5. Holley, AE., Cheeseman, KH. (1993). Measuring free radical reactions in vivo. Br.medbull. Jul; 49(3):494-505.
6. Cheeseman, K.H., Slater, T.F. (1993). An Introduction to Free Radical Biochemistry, British Med. Bull., Jul, 49(3): 481-493.
7. Pelayo Correa. (2007). Jeanmarie Houghton, Carcinogenesis of Helicobacter Pylori, Gastroenterology. 133:659–672.
8. Meister, A., Anderson, M.E. (1983). Glutathione, Ann. Rev. Biochem., 52: 711-760.
9. Güven A, Kaya N. (2005). Determination of Reducte Glutathione(GSH), Glutathione S-Transferase(GST) and Selenium(Se) Levels in Goose Liver Cells with Damage Induced by Carbon Tetrachloride (CCl4) and Ethanol ( C2H5OH). Turk. J Vet Anim Sci, 29: 1233-1238.
10. Wallace, JL., Tıgley, AW. (1995). Review article: new insights into prostaglandins and rnucosal defence. Aliment Pharmacol Ther; 9: 227-235.
11. Annie On On Chan, et al. (2014). Risk factors for gastric cancer. UpToDate 17 April .12. Sadler, T.W. (1996). Langman’s Medikal Embriyoloji, Ceviri editoru Prof.Dr. A.Can Basaklar, Palme yayıncılık, 7.baskı, 236–240.
13. Correa, P., Fox, J., at al, Helicobacter Pylori and Gastric Carcinoma: Serum Antiprevalance in Populations with Contrasting Cancer Risks. Cancer, 66:2569, 1990.
14. Gore R. (1997). Gastrointestinal cancer. Radiol Clin North Am, 35:295-310.
15. McLean MH, El-Omar EM. (2014). Genetic of gastric cancer. Nat Rev Gastroenterol Hepatol [Epub ahead of print] Review. PMID: 25134511.
16. Cevikbas, Uğur(Cevirici Editoru), Gastrik Karsinom: Basic Pathology(Temel Patoloji), Altıncı Edisyon, W.B. Saundres Company, Nobel Tıp Kitapevleri, Yuce Reklam/Yayın/Dağıtım a.s., Philadelphia, Đstanbul, 488-489.
17. Ohkawa H., Ohishi N., at al,. (2000). Assay for Lipid Peroxides in Animal Tissues by Thiobarbituric Acid Reaction” Anal. Biochem., 95: 351-358 (1979).
18. Sedlak J., Lindsay R.H., Estimation of Total Protein-bound and Non-protein Sulfhdryl Groups in Tissue with Ellman’s Reagent, Anal. Biochem., 25: 192-205 (1968).
19. Hristozov, D., Gadjeva, V., at al. (2001). Evaluation of oxidative stress in patients with cancer. Arch. Physiol. Biochem. 109: 331-336.
20. Draper, HH., Hadley, M. (1990). A review of recent studies on the metabolism of exogenous and endogenous malondialdehyde. Xenobiotica’’ 20: 901-7.
21. Romero, FJ., Morell, B., et al. (1998). Lipid peroxidation products and antioxidants in human disease.’’ Environmental Health Perspectives 106: 1229-34.
22. Klarod K, Hongsprabhas P, Khampitak T, Wirasorn K, Kiertiburanakul S, Tangrassameeprasert R, et al. (2015). Serum antioxidant levels and nutritional status in early and advanced stage lung cancer patients. Nutrition. 27:1156-60.
23. Khanzode, SS., Khanzode, SD., at al. (2003). Serum and plasma concentration of oxidant and antioxidants in patients of Helicobacter pylori gastritis and its correlation with gastric cancer. Cancer Lett. 30; 195: 27-31.
24. Ilhan, N., Ilhan, N., at al. (2004). Reactive protein, procalcitonin, interleukin-6, vascular endothelial growth factor and oxidative metabolites in diagnosis of infection and staging in patients with gastric cancer. World J Gastroenterol. 10: 1115-20.
25. Gerber, M., Astre, C., at al. (1998). Tumour progression and oxidant-antioxidant status. Cancer letters. 14: 211-214.
26. Torun, M., Yardım, S., at al, (1995). Serum beta-carotene, vitamin E, vitamin C and malondialdehyde levels in several types Phar.Ther. 20: 259-263. 20.
27. Saez, GT., Bannister, WH., Bannister JV. (1990). Free radicals and thiol compounds-The role of glutathione against free radical toxicity. In: Vina J (ed). Glutathione Metabo Physiological Functions. Boca Raton, FL: CRC Press. Inc, 237-254.
28. Coles, B., Ketterer, B. (1990). The role of glutathione transferases Rev. Biochem. Mol.Biol. 25: 47-70.
29. Daduk, Y. (2006). Mide Kanserinde Talsialik Asit, Glutatyon, Malondialdehit ve Bu Parametrelerin Birbiriyle ve Kanser Evresi ile İliskisinin İncelenmesi Uzmanlık Tezi İstanbul.
30. Choi, MA., Kim, BS., at al. (1999). Serum antioxidative vitamin levels and lipid peroxidation ingastric carcinoma patients. Cancer Letters 136: 89-93.
31. Baskol, M., Baskol, G., vd, 2007. ‘‘Mide Kanserli Hastalarda Oksidan ve Antioksidan Parametreler ve Birbiriyle Đliskileri’’ Turk Klinik Biyokimya Derg. 5(3):83-89.
32. Asaka M, Sugiyama T, Nobuta A et al. (2001). Atrophic gastritis and Intestinal Metaplasia in Japan: Results of a Large Multicenter Study. Helicobacter. 6:294–299.
33. Cerutti PA. (1985). Pro-oxidant states and tumor promotion. Science. 227(4685):375-81.
34. Shamberger RJ. (1972). Increase of peroxidation in carcinogenesis. J Natl Cancer Inst. 48(5):1491-7.

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Details

Primary Language	Turkish
Subjects	Environmental Sciences
Journal Section	Caucasian Journal of Science
Authors	Aysel Güven 0000-0001-7511-7105 Samet Kısaçam This is me
Publication Date	June 28, 2020
Submission Date	November 14, 2019
Acceptance Date	June 27, 2020
Published in Issue	Year 2020 Volume: 7 Issue: 1

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APA	Güven, A., & Kısaçam, S. (2020). Gastrit ve Mide Kanseri Hastalarında Kan Malondialdehit (MDA) ve Redükte Glutatyon(GSH) Düzeylerinin Araştırılması. Caucasian Journal of Science, 7(1), 1-8.

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