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Kistik Ekinokoklu Koyunlarda IFN-γ Seviyelerinin İmmunohistokimyasal Olarak Değerlendirilmesi

Year 2023, Volume: 2 Issue: 1, 37 - 42, 27.06.2023

Emin Karakurt Şemistan Kızıltepe Ayfer Yıldız

Abstract

Güncel çalışmada kistik ekinokokkozisli (KE) koyunların akciğerlerinde parazite karşı gelişen hücresel immun yanıtın detaylandırılması amacıyla sağlıklı hayvanlara kıyasla enfekte hayvanlardaki interferon-gamma (IFN-γ) düzeyleri immunohistokimyasal yöntemlerle değerlendirilmiştir. Bu çalışmanın materyalini Kafkas Üniversitesi Veteriner Fakültesi Patoloji Anabilim Dalı’na rutin tanı amacıyla ölü olarak getirilen ortalama 10 adet koyundan alınan akciğer dokusu örnekleri oluşturmuştur. 10 adet sağlıklı ve herhangi bir patolojik lezyon sergilemeyen koyunlardan alınan akciğer dokusu örnekleri de kontrol amacıyla kullanılmıştır. Dokulardaki histopatolojik değişikliklerin değerlendirilebilmesi amacıyla kesitlere Hematoksilen & Eozin boyaması yapıldı. Avidin-Biotin Peroksidaz-Tekniği kullanılarak IFN-γ ticari antikoru ile üretici firmanın prosedürlerine uygun olarak boyandı. Makroskobik muayenede akciğerin hemen hemen tüm loplarına yayılmış, irili ufaklı oval şekilli kistik yapılar tespit edildi. Akciğerlerin histopatolojik incelemelerinde kistlerin fibröz bir kapsülle çevrelendiği ve fibröz kapsülün hemen alt kısmında konsantirik lamellasyon gösteren kist çeperinin olduğu gözlendi. Kontrol grubuna kıyasla IFN-γ düzeylerinin KE grubunda istatistiki anlamda artış gösterdiği belirlendi. IFN-γ düzeylerindeki bu denli artışın KE gibi paraziter hastalıkların teşhisinde bu belirtecin dikkate değer bir sitokin olduğunu ortaya koymaktadır. Paraziter enfeksiyona karşı geliştirilen hücresel immun yanıtta baskın bir rol oynayan dev hücreleri ve makrofajlar gibi fagositlerde IFN-γ boyamaların çok daha şiddetli olması da bu pro-inflamatuar sitokinin anti-ekinokok tedavisinde etkili olabileceğini göstermektedir.

Keywords

Hücresel Savunma İnterferon-γ Kistik Ekinokokkozis Koyun Sitokin

References

  • Abo-Aziza, F. A. M., Hendawy, S. H. M., Oda, S. S., Aboelsoued, D., & El Shanawany, E. E. (2020). Cell-mediated and humoral immune profile to hydatidosis among naturally infected farm animals. Veterinary World, 13, 214–221. https://doi.org/10.14202/vetworld.2020.214-221
  • Al Malki, J., & Ahmed, N. (2022). Epidemiological and histomorphic studies in sheep infected with hydatid cyst in Taif area. Saudi Journal of Biological Sciences, 29, 886–893. https://doi.org/10.1016/j.sjbs.2021.10.017
  • Al-Qaoud, K. M., & Abdel-Hafez, S. K. (2008). The induction of T helper type 1 response by cytokine gene transfection protects mice against secondary hydatidosis. Parasitology Research, 102, 1151–1155. https:// doi.org/10.1007/s00436-008-0883-x
  • Atmaca H. T. (2022). Determination of macrophage types by immunohistochemical methods in the local immune response to liver hydatid cysts in sheep. Acta Tropica, 229, 106364. https:// doi.org/10.1016/j.actatropica.2022.106364
  • Biranvand, E., Rafiei, A., Beiromvand, M., Amari, A., Bahraini, A., & Motamedfar, A. (2020). Cytokine profiles in peripheral blood mononuclear cells from patients with cystic echinococcosis. Comparative Immunology, Microbiology and Infectious Diseases, 70, 101469. https:// doi.org/10.1016/j.cimid.2020.101469
  • Brik, K., Hassouni, T., Youssir, S., Baroud, S., Elkharrim, K., & Belghyti, D. (2018). Epidemiological study of Echinococcus granulosus in sheep in the Gharb plain (North-West of Morocco). Journal of parasitic diseases : official organ of the Indian Society for Parasitology, 42, 505–510. https:// doi.org/10.1007/s12639-018-1026-7
  • Cengiz, G., & Gonenc, B. (2020). Comparison of molecular and morphological characterization and haplotype analysis of cattle and sheep isolates of cystic echinococcosis. Veterinary Parasitology, 282, 109132. https://doi.org/10.1016/j.vetpar.2020.109132
  • Dvoroznáková, E., Porubcová, J., Snábel, V., & Fedorocko, P. (2008). Imunomodulative effect of liposomized muramyltripeptide phosphatidylethanolamine (L-MTP-PE) on mice with alveolar echinococcosis and treated with albendazole. Parasitology research, 103, 919–929. https://doi.org/10.1007/s00436-008-1077-2
  • Dvoroznáková, E., Porubcová, J., & Sevcíková, Z. (2009). Immune response of mice with alveolar echinococcosis to therapy with transfer factor, alone and in combination with albendazole. Parasitology Research, 105, 1067– 1076. https://doi.org/10.1007/s00436-009-1520-z
  • Gädicke, P., Heath, D., Medina-Brunet, A., Siva-de la Fuente, M. C., Espinoza-Rojas, H., Villaguala-Pacheco, C., Rubilar, M., Cerda, C., Quezada, M., Rojas, D., Henríquez, A., Loyola, M., & Landaeta-Aqueveque, C. (2022). Assessment of the vaccination program against cystic echinococcosis in sheep in the Pehuenche Community of Central Chile. Animals : an open access journal from MDPI, 12, 679. https://doi.org/10.3390/ani12060679
  • Hashemnia, M., Chalechale, A., & Malmir, E. (2019). Pulmonary lesions in slaughtered sheepin Western Iran: gross and histopathological findings. Veterinaria Italiana, 55, 47–56. https://doi.org/10.12834/VetIt.785.3795.3
  • Hidalgo, C., Stoore, C., Pereira, I., Paredes, R., & Alvarez Rojas, C. A. (2020). Multiple haplotypes of Echinococcus granulosus sensu stricto in single naturally infected intermediate hosts. Parasitology Research, 119, 763– 770. https://doi.org/10.1007/s00436-019-06578-2
  • Hidalgo, C., Stoore, C., Baquedano, M. S., Pereira, I., Franco, C., Hernández, M., & Paredes, R. (2021). Response patterns in adventitial layer of Echinococcus granulosus sensu stricto cysts from naturally infected cattle and sheep. Veterinary Research, 52, 66. https://doi.org/10.1186/s13567- 021-00936-8
  • Horta, A. L., Williams, T., Han, B., Ma, Y., Menezes, A. P. J., Tu, V., Talvani, A., Weiss, L. M., & Huang, H. (2020). Resolvin D1 administration is beneficial in Trypanosoma cruzi infection. Infection and Immunity, 88, e00052-20. https://doi.org/10.1128/IAI.00052-20
  • Kesik, H. K., Simsek, S., Kilinc, S. G., & Koroglu, E. (2019). Identification of antigen B (AgB) Gene polymorphism in cattle and sheep isolates of Echinococcus granulosus and investigation of effects on serological diagnosis. Acta Tropica, 199, 105099. https://doi.org/10.1016/ j.actatropica.2019.105099
  • Kittelberger, R., Reichel, M. P., Jenner, J., Heath, D. D., Lightowlers, M. W., Moro, P., Ibrahem, M. M., Craig, P. S., & O'Keefe, J. S. (2002). Evaluation of three enzyme-linked immunosorbent assays (ELISAs) for the detection of serum antibodies in sheep infected with Echinococcus granulosus. Veterinary Parasitology, 110, 57–76. https://doi.org/10.1016/s0304-4017 (02)00308-4
  • Ma, Hai-Long., Zhang, H., Apaer, S., Ma, H., Tuxun, T., Li, Hai-Tao, Cheng, L., Paizula, S., Yimit, Y., Shao, Yin-Mei., Lin, Ren-Yong., & Wen, H. (2016). Impact of intestine mucosal immune barrier in sheep naturally infected with Echinococcus granulosus. International Journal of Clinical and Experimental Pathology, 9, 7069-7077.
  • Ma, X., Wang, L., Zhao, H., Pang, N., Zhang, F., Jiang, T., Liu, X., Mamuti, W., Wen, H., & Ding, J. (2014). Th17 cells are associated with the Th1/ Th2‑cell balance during Echinococcus multilocularis infection. Molecular Medicine Reports, 10, 236–240. https://doi.org/10.3892/mmr.2014.2170
  • Moudgil, A. D., Moudgil, P., Asrani, R. K., & Agnihotri, R. K. (2019). Hydatidosis in slaughtered sheep and goats in India: prevalence, genotypic characterization and pathological studies. Journal of Helminthology, 94, e27. https://doi.org/10.1017/S0022149X18001219
  • Rahdar, M., Rafiei, A., & Valipour-Nouroozi, R. (2020). The combination of Cytokines and albendazole therapy for prophylaxis and treatment of experimental/hydatid cyst. Acta Tropica, 201, 105206. https:// doi.org/10.1016/j.actatropica.2019.105206
  • Shirgholami, Z., Borji, H., Mohebalian, H., & Heidarpour, M. (2021). Effects of Allium sativum on IFN-γ and IL4 concentrations in mice with cystic echinococcosis. Experimental Parasitology, 220, 108042. https:// doi.org/10.1016/j.exppara.2020.108042
  • Tarladaçalışır, T., Eryıldız, C., Tabakçıoğlu, K., & Şakru, N. (2022). Genotyping and identification of Antigen B gene polymorphism of Echinococcus granulosus in Edirne, Thrace, and the first report of Genotype G2 (Tasmanian Sheep Strain) in Turkey. Balkan Medical Journal 39, 401–410. https://doi.org/10.4274/balkanmedj.galenos.2022.2022-5- 68
  • Wang, H., Li, Z., Gao, F., Zhao, J., Zhu, M., He, X., Niu, N., & Zhao, W. (2016). Immunoprotection of recombinant Eg.P29 against Echinococcus granulosus in sheep. Veterinary Research Communications, 40, 73–79. https://doi.org/10.1007/s11259-016-9656-7
  • Xian, J., Zhao, P., Wang, N., Wang, W., Zhang, Y., Meng, J., Ma, X., Wang, Z., & Bo, X. (2021). Molecular characterization of a Tetraspanin TSP11 gene in Echinococcus granulosus and evaluation its immunoprotection in model dogs. Frontiers in Veterinary Science, 8, 759283. https:// doi.org/10.3389/fvets.2021.759283
  • Yang, S., Du, X., Wang, C., Zhang, T., Xu, S., Zhu, Y., Lv, Y., Zhao, Y., Zhu, M., Guo, L., & Zhao, W. (2022). Coding and noncoding RNA expression profiles of spleen CD4+ T lymphocytes in mice with echinococcosis. Contrast Media & Molecular Imaging, 2022, 9742461. https:// doi.org/10.1155/2022/9742461
  • Yildiz, K., Sursal Simsek, N., & Gurcan, I. S. (2022). Determination of extracellular traps structures from sheep polymorphonuclear leukocytes to Echinococcus granulosus protoscoleces. Experimental Parasitology, 239, 108283. https://doi.org/10.1016/j.exppara.2022.108283
  • Zhang, Y., Wang, J., Yang, Q., Li, Z., Xu, X., Chen, C., Hou, Z., He, Q., Sheng, L., Ma, X., & Luo, Y. (2021). Synergism therapeutic and immunoregulatory effects of Albendazole + rAd-mIL-28B against Echinococcosis in experiment-infected mice with protoscoleces. PLoS Neglected Tropical Diseases, 15, e0009927. https://doi.org/10.1371/journal.pntd.0009927

Immunohistochemical Evaluation of IFN-γ levels in sheep with cystic echinococcosis

Year 2023, Volume: 2 Issue: 1, 37 - 42, 27.06.2023

Emin Karakurt Şemistan Kızıltepe Ayfer Yıldız

Abstract

In the present study, with an aim to demonstrate in detail the cellular immune response given to the parasite in the lungs of sheep infected with cystic echinococcosis (CE), a comparative assessment was made of the interferon-gamma (IFN-γ) levels of healthy and infected animals using immunohistochemical methods. The material of this study consisted of lung tissue samples taken from an average of 10 dead sheep brought to Faculty of Veterinary Medicine Department of Pathology for routine diagnosis. Lung tissue samples taken from 10 healthy sheep without any pathological lesions were also used for control purposes. Hematoxylin & Eosin staining was applied to the sections in order to evaluate the histopathological changes in the tissues. Stained with IFN-γ commercial antibody using the Avidin-Biotin Peroxidase-Technique in accordance with the manufacturer's procedures. Macroscopic examination revealed large and small oval-shaped cystic structures that spread to almost all lobes of the lung. In the histopathological examination of the lungs, it was observed that the cysts were surrounded by a fibrous capsule and there was a concentric lamellated cyst wall just below the fibrous capsule. It was determined that IFN-γ levels increased statistically in the CE group compared to the control group. Such an increase in IFN-γ levels reveals that this marker is a remarkable cytokine in the diagnosis of parasitic diseases such as CE. The fact that IFN-γ staining is much more severe in phagocytes such as giant cells and macrophages, which play a dominant role in the cellular immune response developed against parasitic infection, shows that this pro-inflammatory cytokine may be effective in anti-echinococcal therapy.

Keywords

Cellular Defense Cystic Echinococcosis Cytokine Interferon-γ Sheep

References

  • Abo-Aziza, F. A. M., Hendawy, S. H. M., Oda, S. S., Aboelsoued, D., & El Shanawany, E. E. (2020). Cell-mediated and humoral immune profile to hydatidosis among naturally infected farm animals. Veterinary World, 13, 214–221. https://doi.org/10.14202/vetworld.2020.214-221
  • Al Malki, J., & Ahmed, N. (2022). Epidemiological and histomorphic studies in sheep infected with hydatid cyst in Taif area. Saudi Journal of Biological Sciences, 29, 886–893. https://doi.org/10.1016/j.sjbs.2021.10.017
  • Al-Qaoud, K. M., & Abdel-Hafez, S. K. (2008). The induction of T helper type 1 response by cytokine gene transfection protects mice against secondary hydatidosis. Parasitology Research, 102, 1151–1155. https:// doi.org/10.1007/s00436-008-0883-x
  • Atmaca H. T. (2022). Determination of macrophage types by immunohistochemical methods in the local immune response to liver hydatid cysts in sheep. Acta Tropica, 229, 106364. https:// doi.org/10.1016/j.actatropica.2022.106364
  • Biranvand, E., Rafiei, A., Beiromvand, M., Amari, A., Bahraini, A., & Motamedfar, A. (2020). Cytokine profiles in peripheral blood mononuclear cells from patients with cystic echinococcosis. Comparative Immunology, Microbiology and Infectious Diseases, 70, 101469. https:// doi.org/10.1016/j.cimid.2020.101469
  • Brik, K., Hassouni, T., Youssir, S., Baroud, S., Elkharrim, K., & Belghyti, D. (2018). Epidemiological study of Echinococcus granulosus in sheep in the Gharb plain (North-West of Morocco). Journal of parasitic diseases : official organ of the Indian Society for Parasitology, 42, 505–510. https:// doi.org/10.1007/s12639-018-1026-7
  • Cengiz, G., & Gonenc, B. (2020). Comparison of molecular and morphological characterization and haplotype analysis of cattle and sheep isolates of cystic echinococcosis. Veterinary Parasitology, 282, 109132. https://doi.org/10.1016/j.vetpar.2020.109132
  • Dvoroznáková, E., Porubcová, J., Snábel, V., & Fedorocko, P. (2008). Imunomodulative effect of liposomized muramyltripeptide phosphatidylethanolamine (L-MTP-PE) on mice with alveolar echinococcosis and treated with albendazole. Parasitology research, 103, 919–929. https://doi.org/10.1007/s00436-008-1077-2
  • Dvoroznáková, E., Porubcová, J., & Sevcíková, Z. (2009). Immune response of mice with alveolar echinococcosis to therapy with transfer factor, alone and in combination with albendazole. Parasitology Research, 105, 1067– 1076. https://doi.org/10.1007/s00436-009-1520-z
  • Gädicke, P., Heath, D., Medina-Brunet, A., Siva-de la Fuente, M. C., Espinoza-Rojas, H., Villaguala-Pacheco, C., Rubilar, M., Cerda, C., Quezada, M., Rojas, D., Henríquez, A., Loyola, M., & Landaeta-Aqueveque, C. (2022). Assessment of the vaccination program against cystic echinococcosis in sheep in the Pehuenche Community of Central Chile. Animals : an open access journal from MDPI, 12, 679. https://doi.org/10.3390/ani12060679
  • Hashemnia, M., Chalechale, A., & Malmir, E. (2019). Pulmonary lesions in slaughtered sheepin Western Iran: gross and histopathological findings. Veterinaria Italiana, 55, 47–56. https://doi.org/10.12834/VetIt.785.3795.3
  • Hidalgo, C., Stoore, C., Pereira, I., Paredes, R., & Alvarez Rojas, C. A. (2020). Multiple haplotypes of Echinococcus granulosus sensu stricto in single naturally infected intermediate hosts. Parasitology Research, 119, 763– 770. https://doi.org/10.1007/s00436-019-06578-2
  • Hidalgo, C., Stoore, C., Baquedano, M. S., Pereira, I., Franco, C., Hernández, M., & Paredes, R. (2021). Response patterns in adventitial layer of Echinococcus granulosus sensu stricto cysts from naturally infected cattle and sheep. Veterinary Research, 52, 66. https://doi.org/10.1186/s13567- 021-00936-8
  • Horta, A. L., Williams, T., Han, B., Ma, Y., Menezes, A. P. J., Tu, V., Talvani, A., Weiss, L. M., & Huang, H. (2020). Resolvin D1 administration is beneficial in Trypanosoma cruzi infection. Infection and Immunity, 88, e00052-20. https://doi.org/10.1128/IAI.00052-20
  • Kesik, H. K., Simsek, S., Kilinc, S. G., & Koroglu, E. (2019). Identification of antigen B (AgB) Gene polymorphism in cattle and sheep isolates of Echinococcus granulosus and investigation of effects on serological diagnosis. Acta Tropica, 199, 105099. https://doi.org/10.1016/ j.actatropica.2019.105099
  • Kittelberger, R., Reichel, M. P., Jenner, J., Heath, D. D., Lightowlers, M. W., Moro, P., Ibrahem, M. M., Craig, P. S., & O'Keefe, J. S. (2002). Evaluation of three enzyme-linked immunosorbent assays (ELISAs) for the detection of serum antibodies in sheep infected with Echinococcus granulosus. Veterinary Parasitology, 110, 57–76. https://doi.org/10.1016/s0304-4017 (02)00308-4
  • Ma, Hai-Long., Zhang, H., Apaer, S., Ma, H., Tuxun, T., Li, Hai-Tao, Cheng, L., Paizula, S., Yimit, Y., Shao, Yin-Mei., Lin, Ren-Yong., & Wen, H. (2016). Impact of intestine mucosal immune barrier in sheep naturally infected with Echinococcus granulosus. International Journal of Clinical and Experimental Pathology, 9, 7069-7077.
  • Ma, X., Wang, L., Zhao, H., Pang, N., Zhang, F., Jiang, T., Liu, X., Mamuti, W., Wen, H., & Ding, J. (2014). Th17 cells are associated with the Th1/ Th2‑cell balance during Echinococcus multilocularis infection. Molecular Medicine Reports, 10, 236–240. https://doi.org/10.3892/mmr.2014.2170
  • Moudgil, A. D., Moudgil, P., Asrani, R. K., & Agnihotri, R. K. (2019). Hydatidosis in slaughtered sheep and goats in India: prevalence, genotypic characterization and pathological studies. Journal of Helminthology, 94, e27. https://doi.org/10.1017/S0022149X18001219
  • Rahdar, M., Rafiei, A., & Valipour-Nouroozi, R. (2020). The combination of Cytokines and albendazole therapy for prophylaxis and treatment of experimental/hydatid cyst. Acta Tropica, 201, 105206. https:// doi.org/10.1016/j.actatropica.2019.105206
  • Shirgholami, Z., Borji, H., Mohebalian, H., & Heidarpour, M. (2021). Effects of Allium sativum on IFN-γ and IL4 concentrations in mice with cystic echinococcosis. Experimental Parasitology, 220, 108042. https:// doi.org/10.1016/j.exppara.2020.108042
  • Tarladaçalışır, T., Eryıldız, C., Tabakçıoğlu, K., & Şakru, N. (2022). Genotyping and identification of Antigen B gene polymorphism of Echinococcus granulosus in Edirne, Thrace, and the first report of Genotype G2 (Tasmanian Sheep Strain) in Turkey. Balkan Medical Journal 39, 401–410. https://doi.org/10.4274/balkanmedj.galenos.2022.2022-5- 68
  • Wang, H., Li, Z., Gao, F., Zhao, J., Zhu, M., He, X., Niu, N., & Zhao, W. (2016). Immunoprotection of recombinant Eg.P29 against Echinococcus granulosus in sheep. Veterinary Research Communications, 40, 73–79. https://doi.org/10.1007/s11259-016-9656-7
  • Xian, J., Zhao, P., Wang, N., Wang, W., Zhang, Y., Meng, J., Ma, X., Wang, Z., & Bo, X. (2021). Molecular characterization of a Tetraspanin TSP11 gene in Echinococcus granulosus and evaluation its immunoprotection in model dogs. Frontiers in Veterinary Science, 8, 759283. https:// doi.org/10.3389/fvets.2021.759283
  • Yang, S., Du, X., Wang, C., Zhang, T., Xu, S., Zhu, Y., Lv, Y., Zhao, Y., Zhu, M., Guo, L., & Zhao, W. (2022). Coding and noncoding RNA expression profiles of spleen CD4+ T lymphocytes in mice with echinococcosis. Contrast Media & Molecular Imaging, 2022, 9742461. https:// doi.org/10.1155/2022/9742461
  • Yildiz, K., Sursal Simsek, N., & Gurcan, I. S. (2022). Determination of extracellular traps structures from sheep polymorphonuclear leukocytes to Echinococcus granulosus protoscoleces. Experimental Parasitology, 239, 108283. https://doi.org/10.1016/j.exppara.2022.108283
  • Zhang, Y., Wang, J., Yang, Q., Li, Z., Xu, X., Chen, C., Hou, Z., He, Q., Sheng, L., Ma, X., & Luo, Y. (2021). Synergism therapeutic and immunoregulatory effects of Albendazole + rAd-mIL-28B against Echinococcosis in experiment-infected mice with protoscoleces. PLoS Neglected Tropical Diseases, 15, e0009927. https://doi.org/10.1371/journal.pntd.0009927
There are 27 citations in total.

Details

Primary Language English
Subjects Veterinary Sciences
Journal Section Research Articles
Authors

Emin Karakurt 0000-0003-2019-3690

Şemistan Kızıltepe 0000-0003-3727-8893

Ayfer Yıldız 0000-0002-6569-5435

Publication Date June 27, 2023
Submission Date May 4, 2023
Published in Issue Year 2023 Volume: 2 Issue: 1

Cite

APA Karakurt, E., Kızıltepe, Ş., & Yıldız, A. (2023). Immunohistochemical Evaluation of IFN-γ levels in sheep with cystic echinococcosis. Antakya Veteriner Bilimleri Dergisi, 2(1), 37-42.
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